Strain Name:
C57BL/6J-MtgxR5486Btlr/Mmmh
Stock Number:
043047-MU
Citation ID:
RRID:MMRRC_043047-MU
Other Names:
R5486 (G1), C57BL/6J-MtgxR5486Btlr
Major Collection:

Strain Information

Ubr5
Name: ubiquitin protein ligase E3 component n-recognin 5
Synonyms: 4432411E13Rik, Edd1, Edd
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 70790
VEGA: 15
Homologene: 9295
Fgd4
Name: FYVE, RhoGEF and PH domain containing 4
Synonyms: Frabin-gamma, Frabin-beta, Frabin-alpha, Frabin, 9330209B17Rik, ZFYVE6
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224014
Homologene: 26727
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: Megalin, Gp330, D230004K18Rik, b2b1625.2Clo
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Smg7
Name: SMG7 nonsense mediated mRNA decay factor
Synonyms: 9430023P16Rik, Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans)
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226517
Homologene: 32235
Arhgap21
Name: Rho GTPase activating protein 21
Synonyms: 5530401C11Rik, ARHGAP10
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 71435
Homologene: 10822
Snrnp200
Name: small nuclear ribonucleoprotein 200 (U5)
Synonyms: U5-200KD, HELIC2, A330064G03Rik, Ascc3l1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 320632
Homologene: 5859
Mcm3
Name: minichromosome maintenance complex component 3
Synonyms: p1.m, P1, Mcmd
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 17215
HGNC: HGNC:6945
Homologene: 1791
Fam168a
Name: family with sequence similarity 168, member A
Synonyms: 2610030B18Rik, B930006L02Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 319604
Homologene: 18702
Thoc1
Name: THO complex 1
Synonyms: 3110002N20Rik, NMP-84
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225160
VEGA: 18
Homologene: 38012
Bdp1
Name: B double prime 1, subunit of RNA polymerase III transcription initiation factor IIIB
Synonyms: TFIIIB150, TFIIIB90, TAF3B1, TFC5, Tfnr, G630013P12Rik, B130055N23Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 544971
Homologene: 34582
Clic6
Name: chloride intracellular channel 6
Synonyms: CLIC1L, 5730466J16Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 209195
HGNC: HGNC:2065
Homologene: 43154
Pld3
Name: phospholipase D family member 3
Synonyms: Sam-9
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18807
Homologene: 7893
Bod1l
Name: biorientation of chromosomes in cell division 1-like
Synonyms: A230054D04Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 665775
Homologene: 45109
Adam12
Name: ADAM metallopeptidase domain 12
Synonyms: Mltna, ADAM12
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 11489
HGNC: HGNC:190
Homologene: 74862
Hpcal4
Name: hippocalcin-like 4
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 170638
Homologene: 9441
Add3
Name: adducin 3
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 27360
VEGA: 19
HGNC: HGNC:245
Homologene: 40893
Lbr
Name: lamin B receptor
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 98386
HGNC: HGNC:6518
Homologene: 2455
Tecpr2
Name: tectonin beta-propeller repeat containing 2
Synonyms: 4930573I19Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 104859
VEGA: 12
Homologene: 8897
Skor2
Name: SKI family transcriptional corepressor 2
Synonyms: Fussel18, Corl2, Gm7348
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 664805
VEGA: 18
Homologene: 64797
Wdr95
Name: WD40 repeat domain 95
Synonyms: 4930434E21Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 381693
Homologene: 124480
Wdr87-ps
Name: WD repeat domain 87, pseudogene
Synonyms: 4932431P20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 114675
Fat2
Name: FAT atypical cadherin 2
Synonyms: LOC245827, mKIAA0811, Fath2, EMI2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 245827
HGNC: HGNC:3596
Homologene: 1110
Sclt1
Name: sodium channel and clathrin linker 1
Synonyms: 4931421F20Rik, 2610207F23Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 67161
Homologene: 27031
Alpk2
Name: alpha-kinase 2
Synonyms: Hak
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225638
Homologene: 50475
Or52d1
Name: olfactory receptor family 52 subfamily D member 1
Synonyms: GA_x6K02T2PBJ9-6841330-6842268, MOR33-2, Olfr646
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 259058
Homologene: 17481
Nr1d2
Name: nuclear receptor subfamily 1, group D, member 2
Synonyms: Rev-erb beta, RVR
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 353187
HGNC: HGNC:7963
Homologene: 3763
Or7g33
Name: olfactory receptor family 7 subfamily G member 33
Synonyms: GA_x6K02T2PVTD-13277703-13276786, MOR154-1, Olfr853
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258908
HGNC: HGNC:8466
Homologene: 133623
Piwil2
Name: piwi-like RNA-mediated gene silencing 2
Synonyms: Miwi like, mili
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 57746
VEGA: 14
Homologene: 23071
Sh2b2
Name: SH2B adaptor protein 2
Synonyms: Aps
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 23921
Homologene: 10309
Ano3
Name: anoctamin 3
Synonyms: B230324K02Rik, Tmem16c
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228432
Homologene: 57147
Or52n3
Name: olfactory receptor family 52 subfamily N member 3
Synonyms: GA_x6K02T2PBJ9-7509539-7510489, MOR34-7, Olfr665
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258810
Homologene: 110477
Slc22a22
Name: solute carrier family 22 (organic cation transporter), member 22
Synonyms: OAT-PG, BC026439
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 210463
Homologene: 18166
Cyp2d9
Name: cytochrome P450, family 2, subfamily d, polypeptide 9
Synonyms: P450-2D, Cyp2d, testosterone 16alpha-hydroxylase
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 13105
VEGA: 15
Homologene: 86099
Taar7a
Name: trace amine-associated receptor 7A
Synonyms: LOC215856, Taar7a
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 215856
Homologene: 110833
Ccdc7a
Name: coiled-coil domain containing 7A
Synonyms: 4930540C21Rik, 4930517G15Rik, Ccdc7
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 74703
Homologene: 134760
Dnajb12
Name: DnaJ heat shock protein family (Hsp40) member B12
Synonyms: mDj10
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 56709
Homologene: 9749
Cln5
Name: ceroid-lipofuscinosis, neuronal 5
Synonyms: A730075N08Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 211286
VEGA: 14
HGNC: HGNC:2076
Homologene: 4738
Erlec1
Name: endoplasmic reticulum lectin 1
Synonyms: 4933407N01Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 66753
Homologene: 9247
Tex19.2
Name: testis expressed gene 19.2
Synonyms: 4921530G04Rik, Tex19.2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 70956
Homologene: 132323
Acad8
Name: acyl-Coenzyme A dehydrogenase family, member 8
Synonyms: 2310016C19Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 66948
HGNC: HGNC:87
Homologene: 8662
A930009A15Rik
Name: RIKEN cDNA A930009A15 gene
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 77798
Pim3
Name: proviral integration site 3
Synonyms: KID-1/kinase induced by depolarization, Kid1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223775
VEGA: 15
Homologene: 56955
Gm21972
Name: predicted gene 21972
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Xntrpc
Name: Xndc1-transient receptor potential cation channel, subfamily C, member 2 readthrough
Synonyms: Xndr-trpc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 102443351
Homologene: 134407
Gm23482
Name: predicted gene, 23482
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 115485923
VEGA: 13
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 20,814,894 bp
  • A to G, chromosome 1 at 86,137,050 bp
  • A to G, chromosome 1 at 152,846,176 bp
  • A to G, chromosome 1 at 181,818,838 bp
  • T to A, chromosome 2 at 20,855,656 bp
  • T to C, chromosome 2 at 69,437,465 bp
  • T to C, chromosome 2 at 110,745,870 bp
  • C to T, chromosome 2 at 127,233,066 bp
  • A to G, chromosome 3 at 41,647,471 bp
  • A to G, chromosome 4 at 123,190,764 bp
  • A to T, chromosome 5 at 41,807,181 bp
  • A to G, chromosome 5 at 136,232,090 bp
  • A to T, chromosome 5 at 149,596,330 bp
  • C to A, chromosome 7 at 27,533,731 bp
  • T to G, chromosome 7 at 29,536,848 bp
  • T to A, chromosome 7 at 100,834,169 bp
  • GTGTCCTA to GTGTCCTATGTCCTA, chromosome 7 at 102,095,213 bp
  • C to A, chromosome 7 at 104,106,498 bp
  • T to A, chromosome 7 at 104,880,961 bp
  • C to A, chromosome 7 at 133,907,672 bp
  • T to C, chromosome 8 at 128,985,403 bp
  • A to G, chromosome 9 at 19,537,294 bp
  • A to T, chromosome 9 at 26,999,495 bp
  • T to A, chromosome 10 at 23,992,458 bp
  • GC to G, chromosome 10 at 59,892,752 bp
  • G to T, chromosome 10 at 115,579,905 bp
  • A to T, chromosome 11 at 30,935,047 bp
  • A to T, chromosome 11 at 55,253,681 bp
  • A to T, chromosome 11 at 121,117,478 bp
  • A to T, chromosome 12 at 110,933,015 bp
  • T to A, chromosome 13 at 49,709,573 bp
  • T to C, chromosome 13 at 100,098,510 bp
  • A to G, chromosome 14 at 18,206,860 bp
  • T to C, chromosome 14 at 70,401,431 bp
  • T to C, chromosome 14 at 103,076,194 bp
  • C to A, chromosome 15 at 38,008,739 bp
  • A to G, chromosome 15 at 57,263,451 bp
  • T to A, chromosome 15 at 82,452,578 bp
  • T to C, chromosome 15 at 88,863,222 bp
  • A to T, chromosome 16 at 16,475,037 bp
  • A to G, chromosome 16 at 92,529,852 bp
  • A to G, chromosome 18 at 9,992,204 bp
  • A to T, chromosome 18 at 65,294,354 bp
  • A to G, chromosome 18 at 76,858,700 bp
  • G to A, chromosome 19 at 53,244,387 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5486 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043047-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.