Strain Detail Sheet

Strain Name:
C.Cg-Ighb Tg(90k2-T7)3369Khyk/KyhkMmnc
Stock Number:
068103-UNC
Citation ID:
RRID:MMRRC_068103-UNC
Other Names:
f3369 (3609Tg/Vk21c)

Strain Information

Gene Details

Igh
Name: immunoglobulin heavy chain complex
Type: Complex/Cluster/Region
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Non-mutant
Ighb
Name:
Type: Other Genome Feature
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Non-mutant
Tg(90k2-T7)3369Khyk
Name: transgene insertion 3369, Kyoko Hayakawa
Type: Allele
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
90k2-T7
Name: DNA segment, 90k2-T7
Type: DNA Segment
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Transgenic
Genetic Alterations
Introduction of 3609 heavy chain coupled with Vk21c light chain.

Curation note: The data provided for this strain are incomplete and cannot be uniquely tied to genetic entities. The identity of the Ighb allele, for example, is uncertain; it may be a synonym for Ighg2ab (MGI:6515767). Components "3609" and "Vk21c", likewise, are not unique identifiers for genes or other genomic features. The transgene nomenclature rests on the provision of submitter data pointing to DNA segment, 90k2-T7 (NCBI Gene:53097) and a founder ID of 3369. The transgene was randomly inserted. It is unclear what promoter drives the expression.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
    • ES Cell Line
    Not applicable

    Strain Description

    Phenotype
    Mouse 3609 heavy chain + Vk21c light chain which generates anti-thymocyte antibody (ATA)
    MeSH Terms
    • Aging/immunology
    • Animals
    • Autoantibodies/biosynthesis
    • Autoantibodies/blood
    • Autoantibodies/immunology
    • Autoantigens/immunology
    • B-Lymphocyte Subsets/immunology
    • CD5 Antigens/analysis
    • Genes, Immunoglobulin
    • Hybridomas
    • Immunity, Innate
    • Immunologic Surveillance
    • Mice
    • Mice, SCID
    • Mice, Transgenic
    • Receptors, Antigen, B-Cell/immunology
    • Signal Transduction
    • T-Lymphocytes/immunology
    • Thy-1 Antigens/immunology
    • B-Lymphocyte Subsets/cytology
    • B-Lymphocyte Subsets/metabolism
    • Bone Marrow Cells/cytology
    • Bone Marrow Cells/immunology
    • Bone Marrow Cells/metabolism
    • CD5 Antigens/immunology
    • CD5 Antigens/metabolism
    • Cell Differentiation
    • Cell Movement
    • Flow Cytometry
    • Immune Tolerance
    • Immunoglobulin M/analysis
    • Immunoglobulin M/immunology
    • Immunophenotyping
    • Spleen/cytology
    • Spleen/immunology
    • Splenectomy
    • Thy-1 Antigens/analysis
    Strain Development

    The transgenic construct Vh3609mu/Vk21c was injected into C3HxC57BL/6 eggs. Seven founders were identified and bred to CB17 mice (RRID unavailable; please contact Charles River Laboratory or see https://www.criver.com/products-services/find-model/fox-chase-cb17-mouse?region=3611; additional information from MGI: http://www.informatics.jax.org/inbred_strains/mouse/docs/C.shtml), selecting H-2 d/d at the second generation. Founder #3369 was selected and continued to be crossed to CB17 for 33 generations.

    Curation note: "CB17" does not refer to the similarly named line from Taconic (RRID:IMSR_TAC:cb17sc)

    Suggested Control Mice

    CB17 (https://www.criver.com/products-services/find-model/fox-chase-cb17-mouse?region=3611)

    MMRRC Genetic QC

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.

    Research Applications

    • Immunology and Inflammation
    • Models for Human Disease

    Strain Origin

    Donor
    Kyoko Hayakawa, M.D., Fox Chase Cancer Center.
    Primary Reference

    Hayakawa K, Asano M, Shinton SA, Gui M, Allman D, Stewart CL, Silver J, Hardy RR. Positive selection of natural autoreactive B cells. Science. 1999 Jul 2;285(5424):113-6. doi: 10.1126/science.285.5424.113. (Medline PMID: 10390361)

    Hayakawa K, Asano M, Shinton SA, Gui M, Wen LJ, Dashoff J, Hardy RR. Positive selection of anti-thy-1 autoreactive B-1 cells and natural serum autoantibody production independent from bone marrow B cell development. J Exp Med. 2003 Jan 6;197(1):87-99. doi: 10.1084/jem.20021459. (Medline PMID: 12515816)

    Colony and Husbandry Information

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    For more information about this colony's health status contact mmrrc_health@med.unc.edu

    Appearance

    Coat Color
    White
    Eye
    Pink

    Breeding

    MMRRC Breeding System
    Backcross
    Generation

    N33 (CB17; see https://www.criver.com/products-services/find-model/fox-chase-cb17-mouse?region=3611)

    Overall Breeding Performance
    Excellent

    Reproductive Statistics

    NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Undetermined Undetermined
    Homozygotes are fertile: Undetermined Undetermined
    Hetero/Hemizygotes are fertile: Yes Yes
    Age Reproductive Decline: 7 to 9 months 7 to 9 months
    Average litter size
    7 to 9
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    7 to 9

    Order Request Information

    Availability Level

    The availability level for this product has not been determined.

    Conditions of Distribution

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Fees

    - Products for this strain are Not Yet Available for Ordering
    - If you register interest in this strain, you will be notified when it becomes available for ordering.
    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.