Strain Detail Sheet

Strain Name:
C.Cg-Ighb Igh-Jem(14-1H3)Khyk/KyhkMmnc
Stock Number:
068104-UNC
Citation ID:
RRID:MMRRC_068104-UNC
Other Names:
Q52KI (ON25)

Strain Information

Gene Details

Igh-Jem1(14-1H3)Khyk
Name: immunoglobulin heavy chain, joining region; endonuclease-mediated mutation 1, Kyoko Hayakawa
Synonyms: ON25, VHQ52
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Endonuclease-mediated
Igh-J
Name: immunoglobulin heavy chain, joining region
Synonyms: Jh
Type: Complex/Cluster/Region
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Endonuclease-mediated
Igh
Name: immunoglobulin heavy chain complex
Type: Complex/Cluster/Region
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Non-mutant
Ighb
Name:
Type: Other Genome Feature
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Non-mutant
Genetic Alterations
Using zinc finger nucleotide (ZFN)-targeted endonuclease technology, segments 1-4 were replaced with the Q52 VDJH4 rearrangement cloned from the Q52 14-1H3 hybridoma. Mouse line ON25 was used to generate this allele.

Curation note: The data provided for this strain are incomplete and cannot be uniquely tied to genetic entities. The identity of the Ighb allele, for example, is uncertain; it may be a synonym for Ighg2ab (MGI:6515767). Please refer to the cited publications form more information.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
    • ES Cell Line
    Not applicable

    Strain Description

    Phenotype
    B Cell receptor knock-in with non-muscle myosin IIA autoreactivity
    MeSH Terms
    • Animals
    • B-Lymphocytes/pathology
    • Cell Self Renewal
    • Chromosome Deletion
    • Chromosome Disorders
    • Chromosomes, Human, Pair 13
    • Humans
    • Leukemia, Lymphocytic, Chronic, B-Cell/etiology
    • Leukemia, Lymphocytic, Chronic, B-Cell/pathology
    • Mice
    • Nonmuscle Myosin Type IIA/metabolism
    • Receptors, Antigen, B-Cell/metabolism
    • Synteny
    Strain Development
    Two pairs of Fok I heterodimeric ZFNs were designed and provided by Sigma-Aldrich targeting the mouse Ig heavy chain locus in Jh1 and just downstream of Jh4 to delete the Ig Jh locus. The Q52 homologous recombinant donor DNA segment was constructed by adding an 861-bp DNA fragment (left arm) homologous to the sequence upstream of the Jh1 ZFN to a DNA segment containing the Q52 VDJh4 rearrangement, and about 1 kb downstream sequence (right arm) cloned from the Q52 14-1H3 hybridoma. The left homologous arm was obtained by PCR amplification of mouse genomic DNA and cloned. A 2.2 kb DNA fragment flanked by EcoRI sites containing sequence upstream of the Vh promoter, Ig Q52 leader sequence, rearranged V-D-Jh4, and the downstream genomic sequence was cloned from the VhQ52/D/Jh4-mu transgene into pBluescript vector. Then the left arm was ligated to the upstream of Q52 genomic DNA. The recombinant Q52 donor construct was fully sequenced and purified along with the two ZFN mRNA pairs. Mouse zygotes (B6C3F1) were microinjected with a mixture of two pairs of ZFN RNAs and the homologous recombinant donor DNA. Injected zygotes were implanted into pseudopregnant Swiss Webster females and the pups were genotyped to identify homologous recombinants. Since zygotes were generated by an IgMa/IgMb cross, positive lines were then tested for IgM allotype. The ON25 line is the Q52 VDJ inserted on the IgMa chromosomes and backcrossed to CB17 (IgMb) mice, distinguishing the engineered and wild-type IgH alleles.
    Suggested Control Mice

    CB17 (https://www.criver.com/products-services/find-model/fox-chase-cb17-mouse?region=3611)

    MMRRC Genetic QC

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.

    Research Applications

    • Immunology and Inflammation
    • Models for Human Disease

    Strain Origin

    Donor
    Kyoko Hayakawa, M.D., Fox Chase Cancer Center.
    Primary Reference

    Hayakawa K, Formica AM, Colombo MJ, Shinton SA, Brill-Dashoff J, Morse Iii HC, Li YS, Hardy RR. Loss of a chromosomal region with synteny to human 13q14 occurs in mouse chronic lymphocytic leukemia that originates from early-generated B-1 B cells. Leukemia. 2016 Jul;30(7):1510-9. doi: 10.1038/leu.2016.61. Epub 2016 Mar 8. (Medline PMID: 27055869)

    Colony and Husbandry Information

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    For more information about this colony's health status contact mmrrc_health@med.unc.edu

    Appearance

    Coat Color
    White
    Eye
    Pink

    Breeding

    MMRRC Breeding System
    Backcross
    Generation

    N20 (CB17 - see https://www.criver.com/products-services/find-model/fox-chase-cb17-mouse?region=3611 and additional information from MGI: http://www.informatics.jax.org/inbred_strains/mouse/docs/C.shtml)

    Overall Breeding Performance
    Good

    Reproductive Statistics

    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: 7 to 9 months 7 to 9 months
    Average litter size
    4 to 6
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    4 to 6

    Order Request Information

    Availability Level

    The availability level for this product has not been determined.

    Conditions of Distribution

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Fees

    - Products for this strain are Not Yet Available for Ordering
    - If you register interest in this strain, you will be notified when it becomes available for ordering.
    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.