Strain Detail Sheet

Strain Name    :

B6;129-Erbb4tm1Fej/Mmucd

Stock Number :

010439-UCD

Other Names   :

ErbB4 floxed, B6;129-Erbb4tm1Fej/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Erbb4tm1Fej
Name: targeted mutation 1, Frank E Jones
Alteration at locus: Targeted Mutation
Gene Symbol: Erbb4
Name: v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)
Chromosome: 1
Alteration at locus: Targeted Mutation

Genetic Alterations:
In the pFLRT-ERBB4 targeting construct, 2 loxP sites flank exon 2, whereas downstream is the positive neomycin (neo) selection marker flanked by 2 frt. R1 embryonic stem (ES) cells were transfected with the pFLRT-ERBB4-targeting construct, linearized at a unique restriction site in the vector sequence.

Genotype Determination:

ES Cell Line: R1 derived from 129

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Mice homozygous for the targeted mutation are viable and fertile. Homozygous mutant mice show no overt phenotypic abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. Excision of the floxed fragment in mammary glands results in impaired mammary gland differentiation.

Strain of Origin: 129

Strain genetic background: B6;129

Strain Development: R1 ES cells were injected into C57BL/6 blastocysts, resultant chimeras were mated to C57BL/6 females, subsequent pups were sibling mated.

Suggested Control Mice: Wild-type littermates

Research Applications

  • Cancer
  • Cardiovascular
  • Models for Human Disease
  • Neurobiology
  • Research Tools

Strain Origin

Donor: K. C. Kent Lloyd, DVM, Ph.D., University of California, Davis

Primary Reference:

  • Long W, Wagner KU, Lloyd KC, Binart N, Shillingford JM, Hennighausen L, Jones FE. Impaired differentiation and lactational failure of Erbb4-deficient mammary glands identify ERBB4 as an obligate mediator of STAT5. Development. 2003 Nov;130(21):5257-68. Epub 2003 Sep 3. (Medline PMID: 12954715)
  • Golub MS, Germann SL, Lloyd KC. Behavioral characteristics of a nervous system-specific erbB4 knock-out mouse. Behav Brain Res. 2004 Aug 12;153(1):159-70. (Medline PMID: 15219717)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Appearance

Coat color: Black or Agouti

Other:

Breeding

MMRRC Breeding System: Sib mated

Breeding Scheme(s):

  • Homozygous female x Homozygous male

Generation: Unknown

Overall Breeding Performance: Good

Reproductive Statistics

Viability and Fertility:FemaleMale
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes

Age Reproductive Decline: 8 months 10 months

Average litter size: 8

Recommended wean age: 3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
010439-UCD-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.
010439-UCD-SPERMCryo-preserved spermatozoa
$437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity.2
010439-UCD-EMBRYOCryo-preserved embryos
$1,038.00 / $2,621.00
Non-Profit / For-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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Generated: 03/06/2015