Strain Name:
Stock Number:
Citation ID:
Other Names:
ErbB4 floxed, B6;129-Erbb4tm1Fej/Mmcd

Gene Information

Name: erb-b2 receptor tyrosine kinase 4; targeted mutation 1, Frank E Jones
Synonyms: Erbb4Flox, erbB4F
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Targeted Mutation
Name: erb-b2 receptor tyrosine kinase 4
Synonyms: ErbB4, Her4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Targeted Mutation
NCBI: 13869
Homologene: 21084
Genetic Alterations
In the pFLRT-ERBB4 targeting construct, 2 loxP sites flank exon 2, whereas downstream is the positive neomycin (neo) selection marker flanked by 2 frt. R1 embryonic stem (ES) cells were transfected with the pFLRT-ERBB4-targeting construct, linearized at a unique restriction site in the vector sequence.
Genotype Determination
ES Cell Line
R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mice homozygous for the targeted mutation are viable and fertile. Homozygous mutant mice show no overt phenotypic abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. Excision of the floxed fragment in mammary glands results in impaired mammary gland differentiation.
MeSH Terms
  • Animals
  • Caseins/genetics
  • Cell Differentiation/physiology
  • Cloning, Molecular
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism
  • Enzyme Activation
  • Epithelium/anatomy & histology
  • Epithelium/physiology
  • Female
  • Gene Deletion
  • Gene Expression Regulation
  • Humans
  • Lactation/physiology
  • Mammary Glands, Animal/cytology
  • Mammary Glands, Animal/growth & development
  • Mammary Glands, Animal/physiology
  • Mice
  • Milk Proteins/genetics
  • Pregnancy
  • Receptor, Epidermal Growth Factor/genetics
  • Receptor, Epidermal Growth Factor/metabolism
  • Receptor, ErbB-4
  • STAT5 Transcription Factor
  • Signal Transduction/physiology
  • Trans-Activators/genetics
  • Trans-Activators/metabolism
  • Age Factors
  • Animals, Newborn
  • Behavior, Animal/physiology
  • Body Weight/physiology
  • Cues
  • Exploratory Behavior/physiology
  • Genotype
  • Male
  • Maze Learning/physiology
  • Memory/physiology
  • Mice, Knockout/genetics
  • Mice, Knockout/physiology
  • Mortality
  • Motor Activity/physiology
  • Nervous System/growth & development
  • Nervous System/metabolism
  • Neuropsychological Tests
  • Psychomotor Performance/physiology
  • RNA, Messenger/biosynthesis
  • Receptor, Epidermal Growth Factor/deficiency
  • Receptor, Epidermal Growth Factor/physiology
  • Reverse Transcriptase Polymerase Chain Reaction/methods
  • Sex Factors
  • Weaning
Strain Development
R1 ES cells were injected into C57BL/6 blastocysts, resultant chimeras were mated to C57BL/6 females, subsequent pups were sibling mated.
Suggested Control Mice
Wild-type littermates
  • Cancer
  • Cardiovascular
  • Models for Human Disease
  • Neurobiology
  • Research Tools
K. C. Kent Lloyd, DVM, Ph.D., University of California, Davis
Primary Reference
  • Long W, Wagner KU, Lloyd KC, Binart N, Shillingford JM, Hennighausen L, Jones FE. Impaired differentiation and lactational failure of Erbb4-deficient mammary glands identify ERBB4 as an obligate mediator of STAT5. Development. 2003 Nov;130(21):5257-68. Epub 2003 Sep 3. (Medline PMID: 12954715)
  • Golub MS, Germann SL, Lloyd KC. Behavioral characteristics of a nervous system-specific erbB4 knock-out mouse. Behav Brain Res. 2004 Aug 12;153(1):159-70. (Medline PMID: 15219717)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email .
Coat Color
Black or Agouti
MMRRC Breeding System
Sib mated
Breeding Scheme(s)
  • Homozygous female x Homozygous male
Overall Breeding Performance
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 8 months 10 months
Average litter size
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
010439-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / $1,549.67
Non-Profit / For-Profit
Aliquot Approximate quantity3 : 20-40 embryos / aliquot
010439-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $871.90
Non-Profit / For-Profit
Aliquot Approximate quantity2
010439-UCD-RESUS Litter recovered from cryo-archive $3,033.00 / $6,017.01
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.