Strain Name:
C57BL/6J-Unc93b13d/Mmucd
Stock Number:
010466-UCD
Citation ID:
RRID:MMRRC_010466-UCD
Other Names:
3d
Major Collection:

Gene Information

Unc93b13d
Name: unc-93 homolog B1, TLR signaling regulator; triple defect
Synonyms: triple D, 3d, Unc93b1m1Btlr
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
Unc93b1
Name: unc-93 homolog B1, TLR signaling regulator
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 54445
Homologene: 41325
Genetic Alterations
ENU induced point mutation in the cording region.
Genotype Determination
Phenotype
Homozygous: The 3d mutation causes a triple defect of macrophage response: a problem with sensing unmethylated DNA (CpG), dsRNA (poly I:C) and resiquimod. Hence signaling via TLRs 3, 7, and 9 is impaired. The mice are highly susceptible to infection with MCMV, presumably as a result of the multiple sensing defect. More moderate immunodeficiency is observed when mice are challenged with S. aureus, and diminished cytokine production is observed when macrophages from 3d homozygotes are infected with Listeria monocytogenes, particularly variant strains that cannot escape the endosome. In addition, 3d homozygotes show a defect of antigen processing/presentation via the class II pathway.

Heterozygous: Not characterized
Mammalian Phenotype Terms
Allelic Composition: Unc93b13d/Unc93b13d (Genetic Background: C57BL/6-Unc93b13d )

Allelic Composition: Unc93b13d/Unc93b13d (Genetic Background: C57BL/6J-Unc93b13d )

MeSH Terms
  • Animals
  • Antigen Presentation/genetics
  • Cloning, Molecular
  • Endoplasmic Reticulum/metabolism
  • Endosomes/metabolism
  • Histocompatibility Antigens Class II/metabolism
  • Infection/genetics
  • Infection/immunology
  • Membrane Glycoproteins/metabolism
  • Membrane Transport Proteins/genetics
  • Membrane Transport Proteins/immunology
  • Membrane Transport Proteins/metabolism
  • Mice
  • Mice, Inbred C57BL
  • Mice, Mutant Strains
  • Mutation, Missense
  • Phenotype
  • Signal Transduction/genetics
  • Toll-Like Receptor 3/metabolism
  • Toll-Like Receptor 7/metabolism
  • Toll-Like Receptor 9/metabolism
Suggested Control Mice
Wild-type littermates
  • Cell Biology
  • Immunology and Inflammation
  • Models for Human Disease
  • Virology
Donor
Bruce Beutler, M.D., The Scripps Research Institute
Primary Reference
  • Tabeta K, Hoebe K, Janssen EM, Du X, Georgel P, Crozat K, Mudd S, Mann N, Sovath S, Goode J, Shamel L, Herskovits AA, Portnoy DA, Cooke M, Tarantino LM, Wiltshire T, Steinberg BE, Grinstein S, Beutler B. The Unc93b1 mutation 3d disrupts exogenous antigen presentation and signaling via Toll-like receptors 3, 7 and 9. Nat Immunol. 2006 Feb;7(2):156-64. Epub 2006 Jan 15. (Medline PMID: 16415873)
  • For additional information see: Mutagenetix, a catalog of mutations identified in the Beutler Laboratory at The Scripps Research Institute.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu .

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
010466-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
010466-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
010466-UCD-RESUS Litter recovered from cryo-archive $3,033.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.