Loading Mouse GIF
Loading...

Strain Name:
FVB/NJ-Tg(Chrna9-EGFP)1Jnz/Mmucd
Stock Number:
010571-UCD
Citation ID:
RRID:MMRRC_010571-UCD
Other Names:
A9AChR/GFP, FVB/NJ-Tg(Chrna9-EGFP)1Jnz/Mmcd

Strain Information

EGFP
Name: enhanced green fluorescent protein
Type: Gene
Species: Aequorea victoria (jellyfish)
Chromosome:
Chrna9
Name: cholinergic receptor, nicotinic, alpha polypeptide 9
Synonyms: Acra9, 2410015I05Rik, Gm8311
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231252
Homologene: 9729
Tg(Chrna9-EGFP)1Jnz
Name: transgene insertion 1, Jian Zuo
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic (BAC)
Genetic Alterations
Insertion of IRES/GFP in 3'UTR of a9 AChR in a mouse BAC and subsequently derived transgenic mouse.
Genotype Determination
Phenotype
Homozygous: No obvious phenotypes with strong GFP expression in hair cells of the inner ear. Significant noise protection.

Hemizygous: No obvious phenotypes with GFP expression in hair cells of the inner ear. Significant noise protection.
MeSH Terms
  • Animals
  • Chromosomes, Bacterial
  • Female
  • Gene Expression
  • Genes, Reporter
  • Green Fluorescent Proteins
  • Hair Cells, Auditory, Inner/metabolism
  • Luminescent Proteins/genetics
  • Male
  • Mice
  • Mice, Transgenic
  • Receptors, Nicotinic/biosynthesis
  • Receptors, Nicotinic/genetics
  • Recombinant Fusion Proteins/biosynthesis
  • Recombinant Fusion Proteins/genetics
  • Amino Acid Sequence
  • Auditory Pathways
  • Cochlea/physiology
  • Evoked Potentials, Auditory, Brain Stem
  • Hair Cells, Auditory, Outer/chemistry
  • Hearing Loss, Noise-Induced/prevention & control
  • Humans
  • Molecular Sequence Data
  • Organ of Corti/anatomy & histology
  • Organ of Corti/physiology
  • Rats
  • Receptors, Nicotinic/analysis
  • Receptors, Nicotinic/physiology
  • Sequence Alignment
Strain Development
Sib-mating for more than 10 generations since the original description (Zuo et al., PNAS 1999 - PMID:10570205)
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cell Biology
  • Developmental Biology
  • Models for Human Disease
  • Neurobiology
  • Sensorineural
Donor
Jian Zuo, Ph.D., St. Jude Children's Research Hospital
Primary Reference
  • Zuo J, Treadaway J, Buckner TW, Fritzsch B. Visualization of alpha9 acetylcholine receptor expression in hair cells of transgenic mice containing a modified bacterial artificial chromosome. Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):14100-5. (Medline PMID: 10570205)
  • Maison SF, Luebke AE, Liberman MC, Zuo J. Efferent protection from acoustic injury is mediated via alpha9 nicotinic acetylcholine receptors on outer hair cells. J Neurosci. 2002 Dec 15;22(24):10838-46. (Medline PMID: 12486177)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Bred to Homozygosity
No

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
010571-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.