Strain Name:
STOCK Mecp2tm1.1Jae/Mmucd
Stock Number:
000011-UCD
Citation ID:
RRID:MMRRC_000011-UCD

Gene Information

Mecp2tm1.1Jae
Name: methyl CpG binding protein 2; targeted mutation 1.1, Rudolf Jaenisch
Synonyms: Mecp2tm1-1Jae, Mecp1lox
Type: Allele
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Knockout
Mecp2
Name: methyl CpG binding protein 2
Synonyms: Mbd5, 1500041B07Rik, WBP10, D630021H01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Knockout
NCBI: 17257
HGNC: HGNC:6990
Homologene: 3657
Genetic Alterations
Exon 3 of Mecp2 is deleted by cre-mediated recombination between loxP sites which flanked the exon.
Genotype Determination
ES Cell Line
v6.5 derived from (C57BL/6 x 129S4/SvJae)F1
Phenotype
Hemizygous males and homozygous females develop disease (gait ataxia, frequent obesity) around 5 weeks and die as young adults (8 to 12 weeks). Heterozygous females stay healthy for at least 4 months. Mutant brains showed substantial reduction in both weight and neuronal cell size, but no obvious structural defects or signs of neurodegeneration.
Mammalian Phenotype Terms
Allelic Composition: Mecp2tm1.1Jae/Mecp2+ (Genetic Background: involves: 129S4/SvJae * BALB/c * C57BL/6 )

Allelic Composition: Mecp2tm1.1Jae/Mecp2+ (Genetic Background: involves: 129S4/SvJae * C57BL/6 )

MeSH Terms
  • Animals
  • Cell Differentiation
  • Cell Size
  • Central Nervous System/metabolism
  • Central Nervous System/pathology
  • Chromosomal Proteins, Non-Histone
  • CpG Islands
  • DNA-Binding Proteins/deficiency
  • DNA-Binding Proteins/genetics
  • Disease Models, Animal
  • Female
  • Humans
  • Male
  • Methyl-CpG-Binding Protein 2
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Neurons/metabolism
  • Neurons/pathology
  • Phenotype
  • Repressor Proteins
  • Rett Syndrome/genetics
  • Rett Syndrome/metabolism
  • Rett Syndrome/pathology
  • Alleles
  • Diet
  • Genotype
  • Helianthus
  • Lighting
  • Litter Size
  • Methyl-CpG-Binding Protein 2/deficiency
  • Methyl-CpG-Binding Protein 2/genetics
  • Mice, Knockout
  • Reproduction/physiology
  • Reverse Transcriptase Polymerase Chain Reaction
  • Survival
Strain Development
Independent Mecp22 lox ES cells derived from B6129F1 ES cell line were injected into BALB/c host blastocysts to generate chimeras, which were subsequently crossed with BALB/c mice to derive mice bearing a germline transmitted Mecp22 lox allele.

The 1Lox mice were generated from 1Lox/y ES cells (not from crossing the 2Lox animals to a mouse expressing Cre). The loop out was done in cultured (2Lox/Y) ES cells by transient transfection of a Cre expressing plasmid. The ES cell clones were screened by southern to find out the ones that successfully recombined to a 1Lox allele and these were used to generate 1Lox animals..
Suggested Control Mice
Wild-type littermates
Donor
Dr. Rudolf Jaenisch, Whitehead Institute, Massachusetts Institute of Technology
Primary Reference
  • Chen RZ, Akbarian S, Tudor M, Jaenisch R. Deficiency of methyl-CpG binding protein-2 in CNS neurons results in a Rett-like phenotype in mice. Nat Genet. 2001 Mar;27(3):327-31. (Medline PMID: 11242118)
  • Jugloff DG, Logan R, Eubanks JH. Breeding and maintenance of an Mecp2-deficient mouse model of Rett syndrome. J Neurosci Methods. 2006 Jun 30;154(1-2):89-95. Epub 2006 Jan 24. (Medline PMID: 16439027)

Colony and Husbandry Information

Heterzygous females become obese and ill at an early age (approximately 4 months). Hemizygous males have delayed reproductive maturity (approximately 10 weeks), become ill as early as 5 weeks and die by 12 weeks.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu .
Coat Color
Unknown
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
  • Heterozygous Females x Mecp2+/Y Male Littermates
  • Heterozygous Females x Wildtype Males (e.g., C57BL/6J)
Generation
N3 As of: 01/2003
Overall Breeding Performance
Good
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: No N/A
Homozygotes are fertile: No N/A
Hetero/Hemizygotes are fertile: Reduced Reduced
Age Reproductive Decline: 7 Unknown
Bred to Homozygosity
No
Average litter size
8
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
000011-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / $1,549.67
Non-Profit / For-Profit
Aliquot Approximate quantity3 : 20-40 embryos / aliquot
000011-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $871.90
Non-Profit / For-Profit
Aliquot Approximate quantity2
000011-UCD-RESUS Litter recovered from cryo-archive $3,033.00 / $6,017.01
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.