Strain Name:
B6.129S6-S1pr3tm1Rlp/Mmnc
Stock Number:
012038-UNC
Citation ID:
RRID:MMRRC_012038-UNC

Gene Information

S1pr3tm1Rlp
Name: sphingosine-1-phosphate receptor 3; targeted mutation 1, Richard L Proia
Synonyms: S1pr3tm1Cys, S1P3-, S1pr3-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Targeted Mutation
S1pr3
Name: sphingosine-1-phosphate receptor 3
Synonyms: S1P3, LPb3, Edg3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Targeted Mutation
NCBI: 13610
VEGA: 13
HGNC: HGNC:3167
Homologene: 3829
Genetic Alterations
Neo cassette inserted into the single protein coding exon.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    TC1/TC-1 Derived From 129S6/SvEvTac
    Phenotype
    Homozygous: Vascular defects when in combination with other disrupted S1P receptor genes.

    Heterozygous: Same as wild-type.
    Mammalian Phenotype Terms
    Allelic Composition: S1pr3tm1Rlp/S1pr3tm1Rlp (Genetic Background: involves: 129S6/SvEvTac * C57BL/6 )

    MeSH Terms
    • Animals
    • Blood Vessels/anatomy & histology
    • Blood Vessels/embryology
    • Blood Vessels/pathology
    • Embryo, Mammalian/anatomy & histology
    • Embryo, Mammalian/physiology
    • Female
    • Gestational Age
    • Hemorrhage
    • Mice
    • Mice, Knockout
    • Neovascularization, Physiologic
    • Phenotype
    • Pregnancy
    • Receptors, G-Protein-Coupled/genetics
    • Receptors, G-Protein-Coupled/metabolism
    • Receptors, Lysophospholipid
    Strain Development

    Orginally a mixed 129 x C57BL/6 strain. Now backcrossed to C57BL/6.

    A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.

    MMRRC Strain Reference Strains Genomic makeup (Mb)
    Strain Name MMRRC ID # Reference 1 Reference 2 Ref 1 Ref 2 Het Unknown
    B6.129S6-S1pr3tm1Rlp/Mmnc 12038 C57BL/6J 129S6/SvEvTac 2245 106 233 0
    Suggested Control Mice

    Wildtype littermates

    • Cardiovascular
    • Cell Biology
    • Developmental Biology
    • Hematology
    • Immunology and Inflammation
    • Reproduction
    • Research Tools
    Donor
    Richard L. Proia, Ph.D., National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases
    Primary Reference
    Kono M, Mi Y, Liu Y, Sasaki T, Allende ML, Wu YP, Yamashita T, Proia RL. The sphingosine-1-phosphate receptors S1P1, S1P2, and S1P3 function coordinately during embryonic angiogenesis. J Biol Chem. 2004 Jul 9;279(28):29367-73. Epub 2004 May 11. (Medline PMID: 15138255)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu .
    Coat Color
    Should be black, but agouti mice may be present due to the 129 component.
    MMRRC Breeding System
    Sib-mate Homozygotes
    Breeding Scheme(s)
    Sibling
    Overall Breeding Performance
    Good
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Unknown Unknown
    Bred to Homozygosity
    No
    Average litter size
    Six
    Recommended wean age
    Three weeks
    Average Pups Weaned
    Six

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    012038-UNC-EMBRYO Cryo-preserved embryos $1,363.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
    012038-UNC-SPERM Cryo-preserved spermatozoa $548.00 / Non-Profit Aliquot Approximate quantity3
    012038-UNC-RESUS Litter recovered from cryo-archive $2,829.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.