Strain Detail Sheet

Strain Name    :

B6.129S6-S1pr3tm1Rlp/Mmnc

Stock Number :

012038-UNC

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: S1pr3tm1Rlp
Name: targeted mutation 1, Richard L Proia
Alteration at locus: Targeted Mutation
Gene Symbol: S1pr3
Name: sphingosine-1-phosphate receptor 3
Chromosome: 13
Alteration at locus: Targeted Mutation

Genetic Alterations:
neo cassette inserted into the single protein coding exon.

Genotype Determination:

ES Cell Line: TC1/TC-1 derived from 129S6/SvEvTac

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Vascular defects when in combination with other disrupted S1P receptor genes.

Heterozygous phenotype: None


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
S1pr3tm1Rlp/S1pr3tm1Rlp
        involves: 129S6/SvEvTac * C57BL/6
  • cardiovascular system phenotype
    • cardiovascular system phenotype (MGI Ref ID J:106055)
      • no evidence of embryonic hemorrhaging
  • immune system phenotype
    • abnormal B cell physiology (MGI Ref ID J:91161)
      • Marginal zone B cells of the spleen showed reduced responsiveness in chemotaxic tests
      • localization of B cells to the marginal zone of the spleen was normal
      • relocalization of B cells to lymphoid follicles of the spleen after FTY720 stimulation was normal
  • hearing/vestibular/ear phenotype
    • hearing/vestibular/ear phenotype (MGI Ref ID J:121165)
      • at 6 months, homozygotes exhibit normal hearing, and do NOT display loss of spiral ganglion neurons, in contrast to single Edg5tm1Rlp homozygotes and mice doubly homozygous for Edg3tm1Rlp and Edg5tm1Rlp

Strain of Origin: 129S6/SvEvTac

Strain genetic background: C57BL/6

Strain Development: Orginally a mixed 129 x C57BL/6 strain. Now backcrossed to C57BL/6.

Strain Genetic Background Validation: A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.

MMRRC StrainReference StrainsGenomic makeup (Mb)
Strain Name MMRRC ID # Reference 1 Reference 2 Ref 1 Ref 2 Het Unknown
B6.129S6-S1pr3tm1Rlp/Mmnc MMRRC:012038-UNC C57BL/6J 129S6/SvEvTac 2245 106 233 0


Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Cardiovascular
  • Cell Biology
  • Developmental Biology
  • Hematology
  • Immunology and Inflammation
  • Reproduction
  • Research Tools

Strain Origin

Donor: Richard L. Proia, Ph.D., NIH, NIDDK

Primary Reference:

Kono M, Mi Y, Liu Y, Sasaki T, Allende ML, Wu YP, Yamashita T, Proia RL. The sphingosine-1-phosphate receptors S1P1, S1P2, and S1P3 function coordinately during embryonic angiogenesis. J Biol Chem. 2004 Jul 9;279(28):29367-73. Epub 2004 May 11. (Medline PMID: 15138255)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
012038-UNC-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
012038-UNC-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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