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Strain Name:
B6;129S7-Amhr2tm3(cre)Bhr/Mmnc
Stock Number:
014245-UNC
Citation ID:
RRID:MMRRC_014245-UNC

Strain Information

Amhr2tm3(cre)Bhr
Name: anti-Mullerian hormone type 2 receptor; targeted mutation 3, Richard R Behringer
Synonyms: Amhr-2/Cre, Amhr2-IRES-Cre(Bhr), Amhr2cre, Amhr2cre, Amh-mis-Cre, Amhr2-cre
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Knock-In
cre
Name: cre recombinase
Type: Gene
Species: Enterobacteria phage P1 (bacteriophage P1)
Chromosome:
Alteration at locus: Knock-In
Amhr2
Name: anti-Mullerian hormone type 2 receptor
Synonyms: MIS TypeII receptor, MISIIR
Type: Gene
Species: Mouse
Chromosome: 15
Alteration at locus: Knock-In
NCBI: 110542
HGNC: HGNC:465
Homologene: 10746
Genetic Alterations
A cassette containing IRES-cre and an FRT-flanked neo cassette was inserted into exon 5 of the endogenous gene. Expression of functional cre recombinase was detected in both male and female gonads and in the Mullerian duct. Based on phenotypic similarity to previously described Amhr2 deficient mice, this allele is putatively null.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    AB1 derived from 129S7/SvEvBrd-Hprt+
    Phenotype
    Homozygous: Persistent Mullerian Duct Syndrome and infertility in males. Phenotype is similar to Amh and Amhr2 knockouts.

    Heterozygous: None detected.
    Mammalian Phenotype Terms
    Allelic Composition: Amhr2tm3(cre)Bhr/Amhr2tm3(cre)Bhr (Genetic Background: involves: 129S7/SvEvBrd )

    MeSH Terms
    • Activin Receptors, Type I/genetics
    • Activin Receptors, Type I/physiology
    • Alleles
    • Animals
    • Bone Morphogenetic Protein Receptors, Type I
    • Exons
    • In Situ Hybridization
    • Ligands
    • Male
    • Mice
    • Mice, Transgenic
    • Models, Genetic
    • Mullerian Ducts/pathology
    • Mullerian Ducts/physiology
    • Mutation
    • Protein-Serine-Threonine Kinases
    • Receptors, Growth Factor
    • Recombination, Genetic
    • Sex Differentiation
    • Testis/embryology
    • Time Factors
    • Female
    • Follistatin/metabolism
    • Granulosa Cells/metabolism
    • Infertility, Female/etiology
    • Infertility, Female/metabolism
    • Integrases/genetics
    • Integrases/metabolism
    • Ovary/metabolism
    • Cell Proliferation
    • Cholesterol Side-Chain Cleavage Enzyme/genetics
    • Corpus Luteum/abnormalities
    • DNA-Binding Proteins/genetics
    • DNA-Binding Proteins/physiology
    • Gene Expression Regulation
    • Granulosa Cells/physiology
    • Homeodomain Proteins
    • Infertility/genetics
    • Leydig Cells/pathology
    • Leydig Cells/physiology
    • Mice, Knockout
    • Ovary/pathology
    • Ovary/physiology
    • Phosphoproteins/genetics
    • Receptors, Cytoplasmic and Nuclear
    • Receptors, Peptide/genetics
    • Receptors, Transforming Growth Factor beta
    • Sex Differentiation/genetics
    • Steroidogenic Factor 1
    • Testis/abnormalities
    • Testis/cytology
    • Testis/physiology
    • Transcription Factors/genetics
    • Transcription Factors/physiology
    Strain GQC Summary
    The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
    Suggested Control Mice
    Littermates of all relevant genotypes.
    • Apoptosis
    • Cell Biology
    • Developmental Biology
    • Models for Human Disease
      • Persistent Mullerian Duct Syndrome
    • Reproduction
    • Research Tools
    Donor
    Richard Behringer, Ph.D., University of Texas MD Anderson Cancer Center
    Primary Reference
    • Jamin SP, Arango NA, Mishina Y, Hanks MC, Behringer RR. Requirement of Bmpr1a for Mullerian duct regression during male sexual development. Nat Genet. 2002 Nov;32(3):408-10. Epub 2002 Oct 7. (Medline PMID: 12368913)
    • Jorgez CJ, Klysik M, Jamin SP, Behringer RR, Matzuk MM. Granulosa cell-specific inactivation of follistatin causes female fertility defects. Mol Endocrinol. 2004 Apr;18(4):953-67. Epub 2003 Dec 30. (Medline PMID: 14701941)
    • Jeyasuria P, Ikeda Y, Jamin SP, Zhao L, De Rooij DG, Themmen AP, Behringer RR, Parker KL. Cell-specific knockout of steroidogenic factor 1 reveals its essential roles in gonadal function. Mol Endocrinol. 2004 Jul;18(7):1610-9. Epub 2004 Apr 29. (Medline PMID: 15118069)
    Strain Development

    Backcrossed to C57BL/6J.


    Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

    Colony and Husbandry Information

    Donating Investigator states there can be germline activity of this Cre allele, possibly depending on the specific flox allele, genetic background, or other unknown factors.

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.
    Coat Color
    Black and Agouti
    MMRRC Breeding System
    Backcross heterozygous animals to C57BL/6
    Breeding Scheme(s)
    • Backcross
    Overall Breeding Performance
    Good
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Unknown Unknown
    Homozygotes are fertile: Unknown No
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Unknown Unknown
    Average litter size
    8
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    014245-UNC-EMBRYO Cryo-preserved embryos $1,562.00 / $2,593.00
    Non-Profit / For-Profit
    Aliquot Approximate quantity2 : 20-40 embryos / aliquot
    014245-UNC-SPERM Cryo-preserved spermatozoa $520.00 / $863.00
    Non-Profit / For-Profit
    Aliquot Approximate quantity3
    014245-UNC-RESUS Litter recovered from cryo-archive $3,242.00 / $5,382.00
    Non-Profit / For-Profit
    Litter Recovered litter4; additional fees for any special requests.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.


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