Strain Name    : B6;129S7-Amhr2tm3(cre)Bhr/Mmnc

Stock Number : 014245-UNC

Gene Information

(provided by MGI)
Target: Amhr2tm3(cre)Bhr
Name: targeted mutation 3, Richard R Behringer
Chromosome: 15
Alteration at locus: Knock-In
Transgene: cre (Bacteriophage P1)
Name: cre recombinase
Alteration at locus: Knock-In
Target: Amhr2
Name: anti-Mullerian hormone type 2 receptor
Chromosome: 15
Alteration at locus: Knock-In
Genetic Alterations:
IRES-Cre-pA FRT- flanked Pgk-neo-bpA cassette introduced into exon 5 of the Amhr2 locus.

Genotype Determination:

  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line: AB1 derived from 129/SvEvBrd

    Phenotype

    Homozygous phenotype: Persistent Mullerian Duct Syndrome and infertility in males. Phenotype is similar to Amh and Amhr2 knockouts.

    Heterozygous phenotype: None detected.

    Mammalian Phenotype Terms:
    Allelic Composition: Amhr2tm3(cre)Bhr/Amhr2tm3(cre)Bhr (Genetic Background: involves: 129S7/SvEvBrd )

    Strain of Origin: 129/SvEvBrd

    Strain genetic background: 129/SvEvBrd x C57BL/6J mix

    Strain Development: Backcrossed to C57BL/6J.

    Strain Genetic Background Validation: A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.

    MMRRC StrainReference StrainsGenomic makeup (Mb)
    Strain Name MMRRC ID # Reference 1 Reference 2 Ref 1 Ref 2 Het Unknown
    B6;129S7-Amhr2tm3(cre)Bhr MMRRC:014245-UNC C57BL/6J 129S7/AB2.2 2472 0 110 0


    Suggested Control Mice:

    • Wildtype littermates

    • Apoptosis
    • Cell Biology
    • Developmental Biology
    • Models for Human Disease
    • Reproduction
    • Research Tools

    Donor: Richard Behringer, Ph.D., University of Texas M. D. Anderson Cancer Center

    Primary Reference:

    • Jamin SP, Arango NA, Mishina Y, Hanks MC, Behringer RR. Requirement of Bmpr1a for Mullerian duct regression during male sexual development. Nat Genet. 2002 Nov;32(3):408-10. Epub 2002 Oct 7. (Medline PMID: 12368913)
    • Jorgez CJ, Klysik M, Jamin SP, Behringer RR, Matzuk MM. Granulosa cell-specific inactivation of follistatin causes female fertility defects. Mol Endocrinol. 2004 Apr;18(4):953-67. Epub 2003 Dec 30. (Medline PMID: 14701941)
    • Jeyasuria P, Ikeda Y, Jamin SP, Zhao L, De Rooij DG, Themmen AP, Behringer RR, Parker KL. Cell-specific knockout of steroidogenic factor 1 reveals its essential roles in gonadal function. Mol Endocrinol. 2004 Jul;18(7):1610-9. Epub 2004 Apr 29. (Medline PMID: 15118069)

    Colony and Husbandry Information

    Donating Investigator states there can be germline activity of this Cre allele, possibly depending on the specific flox allele, genetic background, or other unknown factors.

    Colony's Current Health Status Report

    For more information about this colony's health status contact mmrrc_health@med.unc.edu

    Coat color: Black

    Other:

    MMRRC Breeding System: Backcross heterozygous animals to C57BL/6

    Breeding Scheme(s):

    • Backcross

    Generation:

    Overall Breeding Performance: Good

    Breeding Comments: Donating investigator states homozygous animals are viable but homozygous males show infertility

    Viability and Fertility:FemaleMaleComments
    Homozygotes are viable: Unknown Unknown
    Homozygotes are fertile: Unknown No
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Unknown Unknown

    Average litter size: 8

    Recommended wean age: 3 weeks

    Order Request Information

    Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at mmrrc@med.unc.edu for more details.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description
    Distribution
    Fee/unit (US $)
    Units Notes
    014245-UNC-HET-FHeterozygous female
    014245-UNC-HET-MHeterozygous male
    014245-UNC-WT-FWild type female
    014245-UNC-WT-MWild type male
    $218.00 / $571.00
    Non-Profit / For-Profit
    Per Mouse The mmrrc@med.unc.edu may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).
    014245-UNC-SPERMCryo-preserved spermatozoa
    $437.00 / $817.00
    Non-Profit / For-Profit
    Aliquot Approximate quantity.2
    014245-UNC-EMBRYOCryo-preserved embryos
    $1,038.00 / $2,621.00
    Non-Profit / For-Profit
    Aliquot Approximate quantity3: 20-40 embryos / aliquot

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.