Strain Detail Sheet

Strain Name    :

B6;129S7-Amhr2tm3(cre)Bhr/Mmnc

Stock Number :

014245-UNC

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Target: Amhr2tm3(cre)Bhr
Name: targeted mutation 3, Richard R Behringer
Alteration at locus: Knock-In
Transgene: cre (Bacteriophage P1)
Name: cre recombinase
Alteration at locus: Knock-In
Target: Amhr2
Name: anti-Mullerian hormone type 2 receptor
Chromosome: 15
Alteration at locus: Knock-In

Genetic Alterations:
IRES-Cre-pA FRT- flanked Pgk-neo-bpA cassette introduced into exon 5 of the Amhr2 locus.

Genotype Determination:

ES Cell Line: AB1 derived from 129/SvEvBrd

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Persistent Mullerian Duct Syndrome and infertility in males. Phenotype is similar to Amh and Amhr2 knockouts.

Heterozygous phenotype: None detected.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype
Amhr2tm3(cre)Bhr/Amhr2+
        involves: 129P2/OlaHsd * 129S/SvEv * C57BL/6 (conditional)
  • mortality/aging
    • early reproductive senescence (MGI Ref ID J:128934)
      • female mice become infertile after 3 to 4 months of breeding
  • reproductive system phenotype
    • early reproductive senescence (MGI Ref ID J:128934)
      • female mice become infertile after 3 to 4 months of breeding
    • male infertility (MGI Ref ID J:128934)
      • slight infertility, with a 16% decrease in litters per month when bred to wild-type females
  • tumorigenesis
    • increased ovary tumor incidence (MGI Ref ID J:128934)
      • after 3 months of age, 100% of female mice have unilateral or bilateral ovarian tumors
      • ovarian carcinoma (MGI Ref ID J:128934)
        • over 70% of female mice have metastic ovarian cancer by 1 year of age
    • testis tumor (MGI Ref ID J:128934)
      • 100% of male mice by 7 months of age exhibit tumors in the testis
      • early stage tumors appear to be sex cord stromal tumors with Sertoli and Leydig cell differentiation
  • cardiovascular system phenotype
    • hemoperitoneum (MGI Ref ID J:128934)
      • found in 50% of female mice over 9 months in age
      • found in about 60% of male mice by 11 months in age


The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Amhr2tm3(cre)Bhr/Amhr2tm3(cre)Bhr
        Background Not Specified
  • reproductive system phenotype
    • male pseudohermaphroditism (MGI Ref ID J:89999)
      • in addition to a normal male reproductive tract, XY mice developed uteri and oviducts
    • secondary sex reversal (MGI Ref ID J:89999)

Strain of Origin: 129/SvEvBrd

Strain genetic background: 129/SvEvBrd x C57BL/6J mix

Strain Development: Backcrossed to C57BL/6J.

Strain Genetic Background Validation: A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.

MMRRC StrainReference StrainsGenomic makeup (Mb)
Strain Name MMRRC ID # Reference 1 Reference 2 Ref 1 Ref 2 Het Unknown
B6;129S7-Amhr2tm3(cre)Bhr MMRRC:014245-UNC C57BL/6J 129S7/AB2.2 2472 0 110 0


Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Apoptosis
  • Cell Biology
  • Developmental Biology
  • Models for Human Disease
  • Reproduction
  • Research Tools

Strain Origin

Donor: Richard Behringer, Ph.D., University of Texas M. D. Anderson Cancer Center

Primary Reference:

  • Jamin SP, Arango NA, Mishina Y, Hanks MC, Behringer RR. Requirement of Bmpr1a for Mullerian duct regression during male sexual development. Nat Genet. 2002 Nov;32(3):408-10. Epub 2002 Oct 7. (Medline PMID: 12368913)
  • Jorgez CJ, Klysik M, Jamin SP, Behringer RR, Matzuk MM. Granulosa cell-specific inactivation of follistatin causes female fertility defects. Mol Endocrinol. 2004 Apr;18(4):953-67. Epub 2003 Dec 30. (Medline PMID: 14701941)
  • Jeyasuria P, Ikeda Y, Jamin SP, Zhao L, De Rooij DG, Themmen AP, Behringer RR, Parker KL. Cell-specific knockout of steroidogenic factor 1 reveals its essential roles in gonadal function. Mol Endocrinol. 2004 Jul;18(7):1610-9. Epub 2004 Apr 29. (Medline PMID: 15118069)

Colony and Husbandry Information

Special Considerations

Donating Investigator states there can be germline activity of this Cre allele, possibly depending on the specific flox allele, genetic background, or other unknown factors.

Health Status Report

Colony's Current Health Status Report

For more information about this colony's health status contact mmrrc_health@med.unc.edu

Appearance

Coat color: Black

Other:

Breeding

MMRRC Breeding System: Backcross heterozygous animals to C57BL/6

Breeding Scheme(s):

  • Backcross

Generation:

Overall Breeding Performance: Good

Breeding Comments: Donating investigator states homozygous animals are viable but homozygous males show infertility

Reproductive Statistics

Viability and Fertility:FemaleMale
Homozygotes are viable: Unknown Unknown
Homozygotes are fertile: Unknown No
Heterozygotes are fertile: Yes Yes

Age Reproductive Decline: Unknown Unknown

Average litter size: 8

Recommended wean age: 3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at mmrrc@med.unc.edu for more details.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $) 		Units Notes
014245-UNC-HET-FHeterozygous female
014245-UNC-HET-MHeterozygous male
014245-UNC-WT-FWild type female
014245-UNC-WT-MWild type male
$218.00 / $571.00
Non-Profit / For-Profit
 Per Mouse The mmrrc@med.unc.edu may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).
014245-UNC-SPERMCryo-preserved spermatozoa
$437.00 / $817.00
Non-Profit / For-Profit
 Aliquot Approximate quantity.2
014245-UNC-EMBRYOCryo-preserved embryos
$1,038.00 / $2,621.00
Non-Profit / For-Profit
 Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.

The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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