Strain Detail Sheet

Strain Name    :

B6.129-Il21rtm1Wjl/Mmucd

Stock Number :

015505-UCD

Other Names   :

IL-21RKO, B6.129-Il21rtm1Wjl/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Il21rtm1Wjl
Name: targeted mutation 1, Warren J Leonard
Alteration at locus: Knockout
Gene Symbol: Il21r
Name: interleukin 21 receptor
Chromosome: 7
Alteration at locus: Knockout

Genetic Alterations:
IL-21RKO mice were generated by using a targeting construct that eliminates the L-21R extracellular and transmembrane domains. See Supplement Figure 1 in citation listed below.

Genotype Determination:

ES Cell Line: Not specified derived from 129

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Impaired B cell antibody responses

Heterozygous phenotype: None


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Il21rtm1Wjl/Il21rtm1Wjl
        involves: 129 * C57BL/6
  • immune system phenotype
    • abnormal CD8-positive T cell physiology (MGI Ref ID J:95221)
      • CD8+ T cell proliferation in vitro is not enhanced in the presence of IL-15 in the same manner as wild-type CD8+ T cells
      • the percentage of antigen-specific CD8+ T cells found in the spleen is a third lower than controls after vaccination
      • the percentage of CD8+ T cells making IFN-gamma one week after vaccination is about half that of wild-type controls
      • defective cytotoxic T cell cytolysis (MGI Ref ID J:95221)
        • the cytotoxic activity of CD8+ T cells is significantly lower than wild-type cells
    • abnormal immunoglobulin level (MGI Ref ID J:80474)
      • decreased IgG1 level (MGI Ref ID J:80474)
        • serum levels of IgG1 in na

          Strain of Origin: 129

          Strain genetic background: C57BL/6J

          Strain Development: No obvious phenotype development. Normal Homozygous breeding

          Suggested Control Mice: Wild-type littermates

Research Applications

  • Apoptosis
  • Cancer
  • Immunology and Inflammation

Strain Origin

Donor: Warren Leonard,M.D., NIH, NHLBI

Primary Reference:

Ozaki K, Spolski R, Feng CG, Qi CF, Cheng J, Sher A, Morse HC 3rd, Liu C, Schwartzberg PL, Leonard WJ. A critical role for IL-21 in regulating immunoglobulin production. Science. 2002 Nov 22;298(5598):1630-4. (Medline PMID: 12446913)

Colony and Husbandry Information

Special Considerations

Pathogen free facility is required.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
015505-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
015505-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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Generated: 04/19/2014