Strain Name:
B6.129-Il21rtm1Wjl/Mmucd
Stock Number:
015505-UCD
Citation ID:
RRID:MMRRC_015505-UCD
Other Names:
IL-21RKO, B6.129-Il21rtm1Wjl/Mmcd

Strain Information

Il21rtm1Wjl
Name: interleukin 21 receptor; targeted mutation 1, Warren J Leonard
Synonyms: IL-21R-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Knockout
Il21r
Name: interleukin 21 receptor
Synonyms: NILR
Type: Gene
Species: Mouse
Chromosome: 7
Alteration at locus: Knockout
NCBI: 60504
HGNC: HGNC:6006
Homologene: 11040
Genetic Alterations
IL-21RKO mice were generated by using a targeting construct that eliminates the L-21R extracellular and transmembrane domains. See Supplement Figure 1 in citation listed below.
Genotype Determination
ES Cell Line
Not specified
Phenotype
Homozygous: Impaired B cell antibody responses

Heterozygous: Same as wild-type
Mammalian Phenotype Terms
Allelic Composition: Il21rtm1Wjl/Il21rtm1Wjl (Genetic Background: involves: 129 * C57BL/6 )

Allelic Composition: Il21rtm1Wjl/Il21rtm1Wjl (Genetic Background: NOD.Cg-Il21rtm1Wjl )

MeSH Terms
  • Animals
  • Antibody-Producing Cells/immunology
  • B-Lymphocytes/immunology
  • CD4-Positive T-Lymphocytes/immunology
  • Cells, Cultured
  • Gene Targeting
  • Genetic Diseases, X-Linked/immunology
  • Humans
  • Immunization
  • Immunoglobulin E/biosynthesis
  • Immunoglobulin G/biosynthesis
  • Immunoglobulins/biosynthesis
  • Immunologic Memory
  • Interferon-gamma/biosynthesis
  • Interleukin-21 Receptor alpha Subunit
  • Interleukin-4/biosynthesis
  • Interleukin-4/physiology
  • Interleukins/physiology
  • Lymphocyte Activation
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Receptors, Interleukin/genetics
  • Receptors, Interleukin/metabolism
  • Receptors, Interleukin-21
  • Severe Combined Immunodeficiency/immunology
  • Signal Transduction
  • T-Lymphocytes/immunology
  • Toxoplasmosis, Animal/immunology
Strain Development
No obvious phenotype development. Normal Homozygous breeding
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Apoptosis
  • Cancer
  • Immunology and Inflammation
Donor
Warren Leonard,M.D., National Institutes of Health, National Heart, Lung, and Blood Institute (NHLBI)
Primary Reference
Ozaki K, Spolski R, Feng CG, Qi CF, Cheng J, Sher A, Morse HC 3rd, Liu C, Schwartzberg PL, Leonard WJ. A critical role for IL-21 in regulating immunoglobulin production. Science. 2002 Nov 22;298(5598):1630-4. (Medline PMID: 12446913)

Colony and Husbandry Information

Pathogen free facility is required.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
015505-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
015505-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.