Strain Name:
B6.Cg-Atrnmg Ay/Mmmh
Stock Number:
000159-MU
Citation ID:
RRID:MMRRC_000159-MU
Major Collection:

Strain Information

Ay
Name: nonagouti; agouti yellow
Synonyms: A(y), Ay
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Spontaneous Mutation
Atrnmg
Name: attractin; mahogany
Synonyms: mg
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Spontaneous Mutation
a
Name: nonagouti
Synonyms: agouti, As, agouti signal protein, ASP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Spontaneous Mutation
NCBI: 50518
HGNC: HGNC:745
Homologene: 1264
Atrn
Name: attractin
Synonyms: Mgca
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Spontaneous Mutation
NCBI: 11990
HGNC: HGNC:885
Homologene: 22542
Genetic Alterations
Possible genotypes of live mice from cryo-resuscitation are:
hom for Atrnmg, hom for a;
hom for Atrnmg, het for Ay;
het for Atrnmg, het for Ay;
het for Atrnmg, hom for a.
Genotype Determination
Phenotype
The mahogany allele reduces the pheomelanic phenotype and the obesity phenotype in mice that are yellow and obese because of mutation at the agouti locus. Mahogany mice also are more active than normal mice, and they have lesions of the brain that increase with age. Black mahogany mice are difficult to differentiate by phenotype from black wild type mice. Therefore, the Ay allele was used for backcrossing mahogany onto C57BL/6J using C57BL/6J-Ay/a mice. The Ay allele is maintained in the stock to differentiate between mahogany and wild-type mice, and also to study the phenotypic and genic interactions between the genic functions of the attracting locus and the agouti locus.
Mammalian Phenotype Terms
Allelic Composition: Ay/Ay (Genetic Background: 101-Ay )

  • mortality/aging
  • other phenotype
Allelic Composition: Ay/Aw (Genetic Background: 129S1.C3-Ay/Aw Chr 19MOLF/Ei )

Allelic Composition: Ay/a (Genetic Background: B6.Cg-Ay/J )

Allelic Composition: Ay/A (Genetic Background: C3HeB/FeJ )

Allelic Composition: Ay/A (Genetic Background: involves: C57BL/6 )

Allelic Composition: Ay/a (Genetic Background: involves: KK )

Allelic Composition: Atrnmg/Atrnmg (Genetic Background: C3H/HeJ.Cg-Atrnmg )

Allelic Composition: Atrnmg/Atrnmg (Genetic Background: LDJ/Le )

MeSH Terms
  • Alleles
  • Alternative Splicing
  • Animals
  • Base Sequence
  • DNA-Binding Proteins/genetics
  • Exons
  • Female
  • Genes, Overlapping
  • Male
  • Mice
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Microphthalmia-Associated Transcription Factor
  • Molecular Sequence Data
  • Mutagenesis
  • Transcription Factors/genetics
  • Transcription, Genetic
  • Eye Abnormalities
  • Genetic Complementation Test
  • Heterozygote
  • Mutation
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Mammalian Pigmentation
  • Developmental Biology
Donor
Lynn Lamoreux, Ph.D., Texas Agricultural and Mechanical University.
Primary Reference

Hallsson JH, Favor J, Hodgkinson C, Glaser T, Lamoreux ML, Magnúsdóttir R, Gunnarsson GJ, Sweet HO, Copeland NG, Jenkins NA, Steingrímsson E. Genomic, transcriptional and mutational analysis of the mouse microphthalmia locus. Genetics. 2000 May;155(1):291-300. (Medline PMID: 10790403)

Steingrímsson E, Arnheiter H, Hallsson JH, Lamoreux ML, Copeland NG, Jenkins NA. Interallelic complementation at the mouse Mitf locus. Genetics. 2003 Jan;163(1):267-76. (Medline PMID: 12586714)

Colony and Husbandry Information

This strain has mutations in two genes. The mahogany allele of the attractin gene is homozygous in this strain (Atrnmg/Atrnmg). The yellow allele of the agouti gene (Ay) is also present in 50% of the offspring (this allele is lethal in the homozygous state so animals are heterozygous for the yellow allele and the nonagouti allele (Ay/a)). Animals heterozygous for the yellow allele & homozygous for the mahogany allele (Ay/a, Atrnmg/Atrnmg) will have a dark brown mahogany coat color and have poor reproduction but allow visualization of the mahogany allele. Animals that do not carry the yellow allele (a/a, Atrnmg/Atrnmg) are black and have good breeding performance but the mahogany coat color is masked by the black coloration of the haircoat.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
MMRRC Breeding System
Sib-mating
Breeding Scheme(s)
  • Homozygous (Atrnmg/Atrnmg) female x Homozygous (Atrnmg/Atrnmg) male
  • The Ay allele still resides within the colony and approximately 1/3 of the animals carry the Ay allele, breeding pairs that are set up such that neither animal carries the Ay allele or only one sex in the breeding cage carries the Ay allele.
Generation
N10+
Overall Breeding Performance
Poor for animals carrying the yellow allele of nonagouti. Good for animals that only carry the mahogany allele of atractin.
Average litter size
4 - 6
Recommended wean age
4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000159-MU-EMBRYO Cryo-preserved embryos $1,038.00 / $2,621.00
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
000159-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
000159-MU-RESUS Litter recovered from cryo-archive $2,016.00 / $4,103.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.