Strain Detail Sheet

Strain Name    :

C57BL/6-MitfMi-ws/Mmmh

Stock Number :

000166-MU

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: MitfMi-ws
Name: white spot
Chromosome: 6; 40.0 cM
Alteration at locus: Radiation Induced
Gene Symbol: Mitf
Name: microphthalmia-associated transcription factor
Chromosome: 6
Alteration at locus: Radiation Induced

Genetic Alterations:
A 4280 bp genomic deletion that starts in a intron 170 bp upstream of the exon 2 splice acceptor site and ends 767 bp downstream of the exon 4 splice donor site.

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Heterozygous mice have a large white belly spot and more white on their tail (about half of the tail is white) when compared to C57BL/6J mice. Homozygous mice are white with eyes that are near normal size.

White Spotting mutants

Characteristics of the Mitf Locus:
Microphthalmia (Mitf) locus encodes the basic-helix-loop-helix-leucine zipper protein transcription factor MITF (Microphthalmia associated transcription factor). (9, 26, 27). Tissues/organs that may be affected (depending upon the allele) include pigmentation of the skin, pigmentation and development of the eye and ear, mast cells, osteoclasts, and reproductive cells. Mitf is an important transcription factor that functions to initiate transcription at several other loci within the pigment cell, including tyrosinase (tyr) and tyrosinase-related protein I (TyrpI.) and also regulates Bcl2. In other cell types, for example in osteoclasts and mast cells, Mitf functions as transcription factor of several cell-type specific proteins. There is no evidence that the size of white spots on mice mutant at Mitf are influenced by pheomelanin. However, mice that are both recessive yellow and Miwh/+ in genotype are much more eumelanic than mice that are yellow and +/+ (Lamoreux, 1981). Strain background does not appear to influence size or location of spots in mice mutant at Mitf.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype
MitfMi-ws/Mitf+
        C57BL/6
  • pigmentation phenotype
    • abnormal coat/hair pigmentation (MGI Ref ID J:13417)
      • white spotting but no coat dilution
      • white spotting (MGI Ref ID J:13417)
        • belly spot (MGI Ref ID J:13417)
          • diamond shaped pattern is most common
  • integument phenotype
    • abnormal coat/hair pigmentation (MGI Ref ID J:13417)
      • white spotting but no coat dilution
      • white spotting (MGI Ref ID J:13417)
        • belly spot (MGI Ref ID J:13417)
          • diamond shaped pattern is most common
MitfMi-ws/MitfMi-ws
        C57BL/6
  • pigmentation phenotype
    • abnormal coat/hair pigmentation (MGI Ref ID J:13417)
      • absent coat pigmentation (MGI Ref ID J:13417)
        • homozygotes are white
    • abnormal eye pigmentation (MGI Ref ID J:13417)
      • reduced eye pigmentation (MGI Ref ID J:13417)
  • vision/eye phenotype
    • abnormal eye morphology (MGI Ref ID J:13417)
      • abnormal eye development (MGI Ref ID J:13417)
      • abnormal eye pigmentation (MGI Ref ID J:13417)
        • reduced eye pigmentation (MGI Ref ID J:13417)
      • abnormal eye size (MGI Ref ID J:13417)
        • microphthalmia (MGI Ref ID J:13417)
          • found in some, but not all mice
  • integument phenotype
    • abnormal coat/hair pigmentation (MGI Ref ID J:13417)
      • absent coat pigmentation (MGI Ref ID J:13417)
        • homozygotes are white

Strain of Origin: C57BL/6

Strain genetic background: C57BL/6J

Strain Development: gamma-irradiation induced mutation

Suggested Control Mice:

  • Wildtype littermates
  • C57BL/6

Research Applications

  • Mammalian Pigmentation
  • Developmental Biology

Strain Origin

Donor: Lynn Lamoreux, Ph.D., Texas A & M University

Primary Reference:

  • Miller DS. Coat color and behavior mutations in inbred mice under chronic low-level gamma-irradiation. Radiat Res. 1963; 19():184-185 . (Medline PMID: Not Available)
  • Hodgkinson CA, Moore KJ, Nakayama A, Steingrimsson E, Copeland NG, Jenkins NA, Arnheiter H. Mutations at the mouse microphthalmia locus are associated with defects in a gene encoding a novel basic-helix-loop-helix-zipper protein. Cell. 1993 Jul 30;74(2):395-404. (Medline PMID: 8343963)
  • Hallsson JH, Favor J, Hodgkinson C, Glaser T, Lamoreux ML, Magnusdottir R, Gunnarsson GJ, Sweet HO, Copeland NG, Jenkins NA, Steingrimsson E. Genomic, transcriptional and mutational analysis of the mouse microphthalmia locus. Genetics. 2000 May;155(1):291-300. (Medline PMID: 10790403)
  • Steingrimsson E, Arnheiter H, Hallsson JH, Lamoreux ML, Copeland NG, Jenkins NA. Interallelic complementation at the mouse Mitf locus. Genetics. 2003 Jan;163(1):267-76. (Medline PMID: 12586714 )
  • Moller A, Exp Eye Res 2004;78():837-848 (Medline PMID: Not Available)

Colony and Husbandry Information

Special Considerations

Feed mouse chow that contains 9 percent fat for breeding animals.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat color: Heterozygous: black, Homozygous: white (see description under phenotype)

Other:


Breeding

MMRRC Breeding System: Sibling or Backcross

Breeding Scheme(s):

  • Heterozygous Female x Homozygous Male
  • Wildtype female x Homozygous Male

Generation: N>10

Overall Breeding Performance: Good if homozygous males are mated to either heterozygous or wild type females. Poor if homozygous males are mated to homozygous females.

Reproductive Statistics

Viability and Fertility:FemaleMale
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Poor Yes
Heterozygotes are fertile: Yes Yes
 
Age Reproductive Decline: Unknown Unknown

Average litter size: 6

Recommended wean age: 4 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
000166-MU-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.
000166-MU-SPERMCryo-preserved spermatozoa
$437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity.2
000166-MU-EMBRYOCryo-preserved embryos
$1,038.00 / $2,621.00
Non-Profit / For-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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