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Strain Detail Sheet

Strain Name:
C57BL/6-MitfMi-ws/Mmmh
Stock Number:
000166-MU
Citation ID:
RRID:MMRRC_000166-MU
Other Names:
microphthalmia transcription factor
Major Collection:
Mammalian Pigmentation

Strain Information

Gene Details

MitfMi-ws
Name: melanogenesis associated transcription factor; white spot
Synonyms: miws
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Radiation Induced
Mitf
Name: melanogenesis associated transcription factor
Synonyms: mi, wh, BCC2, bHLHe32, Gsfbcc2
Type: Gene
Species: Mouse
Chromosome: 6
Alteration at locus: Radiation Induced
NCBI: 17342
HGNC: HGNC:7105
Homologene: 4892

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
A 4280 bp genomic deletion that starts in a intron 170 bp upstream of the exon 2 splice acceptor site and ends 767 bp downstream of the exon 4 splice donor site.
Genotype Determination

Strain Description

Phenotype
Heterozygous mice have a large white belly spot and more white on their tail (about half of the tail is white) when compared to C57BL/6J mice. Homozygous mice are white with eyes that are near normal size.

White Spotting mutants

Characteristics of the Mitf Locus:
Microphthalmia (Mitf) locus encodes the basic-helix-loop-helix-leucine zipper protein transcription factor MITF (Microphthalmia associated transcription factor). (9, 26, 27). Tissues/organs that may be affected (depending upon the allele) include pigmentation of the skin, pigmentation and development of the eye and ear, mast cells, osteoclasts, and reproductive cells. Mitf is an important transcription factor that functions to initiate transcription at several other loci within the pigment cell, including tyrosinase (tyr) and tyrosinase-related protein I (TyrpI.) and also regulates Bcl2. In other cell types, for example in osteoclasts and mast cells, Mitf functions as transcription factor of several cell-type specific proteins. There is no evidence that the size of white spots on mice mutant at Mitf are influenced by pheomelanin. However, mice that are both recessive yellow and Miwh/+ in genotype are much more eumelanic than mice that are yellow and +/+ (Lamoreux, 1981). Strain background does not appear to influence size or location of spots in mice mutant at Mitf.
Mammalian Phenotype Terms
Allelic Composition: MitfMi-ws/MitfMi-ws (Genetic Background: C57BL/6 )
Allelic Composition: MitfMi-ws/Mitf+ (Genetic Background: C57BL/6 )
MeSH Terms
  • Alleles
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA-Binding Proteins/genetics
  • Gene Expression
  • Genetic Complementation Test
  • Humans
  • Leucine Zippers
  • Mice
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Mice, Transgenic/embryology
  • Microphthalmia-Associated Transcription Factor
  • Microphthalmos/genetics
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Protein Conformation
  • Protein Structure, Secondary
  • Sequence Homology, Amino Acid
  • Tissue Distribution
  • Transcription Factors/genetics
  • Vitiligo/genetics
  • Waardenburg Syndrome/genetics
  • Alternative Splicing
  • Exons
  • Female
  • Genes, Overlapping
  • Male
  • Mutagenesis
  • Transcription, Genetic
  • Eye Abnormalities
  • Heterozygote
  • Mutation
Strain Development
gamma-irradiation induced mutation
Suggested Control Mice
  • Wildtype littermates
  • C57BL/6

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

Research Applications

  • Mammalian Pigmentation
  • Developmental Biology

Strain Origin

Donor
Lynn Lamoreux, Ph.D., Texas A & M University
Primary Reference
  • Miller DS. Coat color and behavior mutations in inbred mice under chronic low-level gamma-irradiation. Radiat Res. 1963; 19():184-185 . (Medline PMID: Not Available)
  • Hodgkinson CA, Moore KJ, Nakayama A, Steingrimsson E, Copeland NG, Jenkins NA, Arnheiter H. Mutations at the mouse microphthalmia locus are associated with defects in a gene encoding a novel basic-helix-loop-helix-zipper protein. Cell. 1993 Jul 30;74(2):395-404. (Medline PMID: 8343963)
  • Hallsson JH, Favor J, Hodgkinson C, Glaser T, Lamoreux ML, Magnusdottir R, Gunnarsson GJ, Sweet HO, Copeland NG, Jenkins NA, Steingrimsson E. Genomic, transcriptional and mutational analysis of the mouse microphthalmia locus. Genetics. 2000 May;155(1):291-300. (Medline PMID: 10790403)
  • Steingrimsson E, Arnheiter H, Hallsson JH, Lamoreux ML, Copeland NG, Jenkins NA. Interallelic complementation at the mouse Mitf locus. Genetics. 2003 Jan;163(1):267-76. (Medline PMID: 12586714 )
  • Moller A, Exp Eye Res 2004;78():837-848 (Medline PMID: Not Available)

Colony and Husbandry Information

Special Considerations

Feed mouse chow that contains 9 percent fat for breeding animals.

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Heterozygous: black, Homozygous: white (see description under phenotype)

Breeding

MMRRC Breeding System
Sibling or Backcross
Breeding Scheme(s)
  • Heterozygous Female x Homozygous Male
  • Wildtype female x Homozygous Male
Generation
N>10
Overall Breeding Performance
Good if homozygous males are mated to either heterozygous or wild type females. Poor if homozygous males are mated to homozygous females.

Reproductive Statistics

Average litter size
6
Recommended wean age
4 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
000166-MU-EMBRYO Cryo-preserved embryos $1,038.00 / $2,621.00
Non-Profit / For-Profit		 Aliquot Approximate quantity2 : 20-40 embryos / aliquot
000166-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit		 Aliquot Approximate quantity3
000166-MU-RESUS Litter recovered from cryo-archive $2,016.00 / $4,103.00
Non-Profit / For-Profit		 Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.