Allele Symbol: Kcnj10tm1Lst
Name: targeted mutation 1, Henry A Lester
Alteration at locus: Targeted Mutation
Gene Symbol: Kcnj10
Name: potassium inwardly-rectifying channel, subfamily J, member 10
Alteration at locus: Targeted Mutation
ES Cell Line: CJ7
Homozygous: Key points from published papers.
Kir4.1 knockout mice die at P11-18. Mice display marked motor
impairment, and the cellular basis is hypomyelination in the
spinal cord, accompanied by severe spongiform vacuolation, axonal
swellings, and degeneration. Dysmyelination is extensive in many
parts of the brain. The brain stem is most affected and during the
first 2 weeks of life, the changes extend forward to the frontal parts
like corpus callosum.
The slow PIII response of the light-evoked electroretinograms (ERG),
which is generated by K1 fluxes through Muller cells, is totally absent
in retinas from Kir4.1 knockout mice.
Mice lacking the KCNJ10 (Kir4.1) K+ channel in strial intermediate cells
did not generate an endocochlear potential. Endolymph volume and K+
concentration were reduced. Startle response experiments suggest that
Kir4.1 knockout mice are profoundly deaf, whereas Kir4.1+/- mice react
like wild type mice to acoustic stimuli. In Kir4.1 knockout mice, the
Reissners membrane is collapsed, the tectorial membrane is swollen,
and spiral ganglion neuron central processes degenerate. In the
vestibular ganglia,neuronal cell death with apoptotic features
Heterozygous: show little phenotype.
Strain of Origin: mixed 129SV and C57BL/6 mixed 129SV and C57BL/6 (> 6 generations)
Strain genetic background: mixed 129SV and C57BL/6 (> 6 generations)
Suggested Control Mice:
Donor: Henry Lester, Ph.D., California Institute of Technology
Kofuji P, Ceelen P, Zahs KR, Surbeck LW, Lester HA, Newman EA. Genetic inactivation of an inwardly rectifying potassium channel (Kir4.1 subunit) in mice: phenotypic impact in retina. J Neurosci. 2000 Aug 1;20(15):5733-40. (Medline PMID: 10908613)
Coat color: Black
MMRRC Breeding System: Back-cross
Overall Breeding Performance: Normal
|Viability and Fertility:||Female||Male||Comments|
|Homozygotes are viable:||No||No||Knockout mice die at P11-18. Mice display marked motor impairment.|
|Homozygotes are fertile:||No||No|
|Heterozygotes are fertile:||Yes||Yes|
|Age Reproductive Decline:||Unknown||Unknown|
Average litter size: 6 - 8
Recommended wean age: 3 weeks
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.