Strain Name:
B6.129S1-Kcnj10tm1Lst/Mmnc
Stock Number:
000209-UNC
Citation ID:
RRID:MMRRC_000209-UNC

Strain Information

Kcnj10tm1Lst
Name: potassium inwardly-rectifying channel, subfamily J, member 10; targeted mutation 1, Henry A Lester
Synonyms: Kir4.1-, Kcnj10tm1Pkj
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Targeted Mutation
Kcnj10
Name: potassium inwardly-rectifying channel, subfamily J, member 10
Synonyms: Kir1.2, BIRK-1, BIR10, Kir4.1, Kir4.1
Type: Gene
Species: Mouse
Chromosome: 1
Alteration at locus: Targeted Mutation
NCBI: 16513
HGNC: HGNC:6256
Homologene: 1689
Genetic Alterations
A neomycin resistance gene replaces amino acids 33­266 in the Kir4.1 deduced primary sequence and thus contains the putative transmembrane domains and part of the C terminus of the Kir4.1 polypeptide.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    CJ7 derived from 129S1/Sv-Oca2+ Tyr+ Kitl+
    Phenotype
    Homozygous: Key points from published papers. Kir4.1 knockout mice die at P11-18. Mice display marked motor impairment, and the cellular basis is hypomyelination in the spinal cord, accompanied by severe spongiform vacuolation, axonal swellings, and degeneration. Dysmyelination is extensive in many parts of the brain. The brain stem is most affected and during the first 2 weeks of life, the changes extend forward to the frontal parts like corpus callosum. The slow PIII response of the light-evoked electroretinograms (ERG), which is generated by K1 fluxes through Muller cells, is totally absent in retinas from Kir4.1 knockout mice. Mice lacking the KCNJ10 (Kir4.1) K+ channel in strial intermediate cells did not generate an endocochlear potential. Endolymph volume and K+ concentration were reduced. Startle response experiments suggest that Kir4.1 knockout mice are profoundly deaf, whereas Kir4.1+/- mice react like wild type mice to acoustic stimuli. In Kir4.1 knockout mice, the Reissners membrane is collapsed, the tectorial membrane is swollen, and spiral ganglion neuron central processes degenerate. In the vestibular ganglia,neuronal cell death with apoptotic features is observed.

    Heterozygous: show little phenotype.
    Mammalian Phenotype Terms
    Allelic Composition: Kcnj10tm1Lst/Kcnj10tm1Lst (Genetic Background: involves: 129S1/Sv * C57BL/6J )

    Allelic Composition: Kcnj10tm1Lst/Kcnj10+ (Genetic Background: involves: 129S1/Sv * C57BL/6J )

    MeSH Terms
    • Amino Acid Sequence
    • Animals
    • Antibodies
    • Astrocytes/chemistry
    • Astrocytes/physiology
    • Electric Impedance
    • Electroretinography
    • Gene Expression/physiology
    • Ion Channel Gating/genetics
    • Membrane Potentials/physiology
    • Mice
    • Mice, Inbred C57BL
    • Mice, Knockout
    • Molecular Sequence Data
    • Patch-Clamp Techniques
    • Phenotype
    • Potassium Channels/genetics
    • Potassium Channels/immunology
    • Potassium Channels/metabolism
    • Potassium Channels, Inwardly Rectifying
    • RNA, Messenger/analysis
    • Rabbits
    • Retina/chemistry
    • Retina/cytology
    • Retina/physiology
    Suggested Control Mice
    • Wildtype littermates
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Apoptosis
    • Developmental Biology
    • Models for Human Disease
    • Neurobiology
    • Research Tools
    • Sensorineural
    Donor
    Henry Lester, Ph.D., California Institute of Technology
    Primary Reference
    Kofuji P, Ceelen P, Zahs KR, Surbeck LW, Lester HA, Newman EA. Genetic inactivation of an inwardly rectifying potassium channel (Kir4.1 subunit) in mice: phenotypic impact in retina. J Neurosci. 2000 Aug 1;20(15):5733-40. (Medline PMID: 10908613)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.
    Coat Color
    Black
    MMRRC Breeding System
    Back-cross
    Breeding Scheme(s)
    • Heterozygous female x C57BL/6 male
      Heterozygous male x C57BL/6 female 
    Generation
    Unknown
    Overall Breeding Performance
    Normal
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: No No Knockout mice die at P11-18. Mice display marked motor impairment.
    Homozygotes are fertile: No No
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Unknown Unknown
    Average litter size
    6 - 8
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    000209-UNC-SPERM Cryo-preserved spermatozoa $564.00 / $937.00
    Non-Profit / For-Profit
    Aliquot Approximate quantity3
    000209-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / $4,837.00
    Non-Profit / For-Profit
    Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.