Strain Name    : B6.129S1-Kcnj10tm1Lst/Mmnc

Stock Number : 000209-UNC

Gene Information

(provided by MGI)
Allele Symbol: Kcnj10tm1Lst
Name: targeted mutation 1, Henry A Lester
Chromosome: 1
Alteration at locus: Targeted Mutation
Gene Symbol: Kcnj10
Name: potassium inwardly-rectifying channel, subfamily J, member 10
Chromosome: 1
Alteration at locus: Targeted Mutation
Genetic Alterations:
A neomycin resistance gene replaces amino acids 33­266 in the Kir4.1 deduced primary sequence and thus contains the putative transmembrane domains and part of the C terminus of the Kir4.1 polypeptide.

Genotype Determination:

  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line:

    Phenotype

    Homozygous: Key points from published papers. Kir4.1 knockout mice die at P11-18. Mice display marked motor impairment, and the cellular basis is hypomyelination in the spinal cord, accompanied by severe spongiform vacuolation, axonal swellings, and degeneration. Dysmyelination is extensive in many parts of the brain. The brain stem is most affected and during the first 2 weeks of life, the changes extend forward to the frontal parts like corpus callosum. The slow PIII response of the light-evoked electroretinograms (ERG), which is generated by K1 fluxes through Muller cells, is totally absent in retinas from Kir4.1 knockout mice. Mice lacking the KCNJ10 (Kir4.1) K+ channel in strial intermediate cells did not generate an endocochlear potential. Endolymph volume and K+ concentration were reduced. Startle response experiments suggest that Kir4.1 knockout mice are profoundly deaf, whereas Kir4.1+/- mice react like wild type mice to acoustic stimuli. In Kir4.1 knockout mice, the Reissners membrane is collapsed, the tectorial membrane is swollen, and spiral ganglion neuron central processes degenerate. In the vestibular ganglia,neuronal cell death with apoptotic features is observed.

    Heterozygous: show little phenotype.

    Mammalian Phenotype Terms:
    Allelic Composition: Kcnj10tm1Lst/Kcnj10tm1Lst (Genetic Background: involves: 129S1/Sv * C57BL/6J )

    Allelic Composition: Kcnj10tm1Lst/Kcnj10+ (Genetic Background: involves: 129S1/Sv * C57BL/6J )

    Strain of Origin: mixed 129SV and C57BL/6 mixed 129SV and C57BL/6 (> 6 generations)

    Strain genetic background: mixed 129SV and C57BL/6 (> 6 generations)

    Suggested Control Mice:

    • Wildtype littermates

    • Apoptosis
    • Developmental Biology
    • Models for Human Disease
    • Neurobiology
    • Research Tools
    • Sensorineural

    Donor: Henry Lester, Ph.D., California Institute of Technology

    Primary Reference:

    Kofuji P, Ceelen P, Zahs KR, Surbeck LW, Lester HA, Newman EA. Genetic inactivation of an inwardly rectifying potassium channel (Kir4.1 subunit) in mice: phenotypic impact in retina. J Neurosci. 2000 Aug 1;20(15):5733-40. (Medline PMID: 10908613)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Coat color: Black

    Other:

    MMRRC Breeding System: Back-cross

    Breeding Scheme(s):

    • Heterozygous female x C57BL/6 male
      Heterozygous male x C57BL/6 female 

    Generation: Unknown

    Overall Breeding Performance: Normal

    Viability and Fertility:FemaleMaleComments
    Homozygotes are viable: No No Knockout mice die at P11-18. Mice display marked motor impairment.
    Homozygotes are fertile: No No
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Unknown Unknown

    Average litter size: 6 - 8

    Recommended wean age: 3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description
    Distribution
    Fee/unit (US $)
    Units Notes
    000209-UNC-RESUSLitter recovered from cryo-archive
    $2,022.00 / $4,109.00
    Non-Profit / For-Profit
    Litter Recovered litter1; additional fees for any special requests.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.