Strain Name:
STOCK Tg(Drd2-EGFP)S118Gsat/Mmnc
Stock Number:
000230-UNC
Citation ID:
RRID:MMRRC_000230-UNC
Major Collection:

Gene Information

Drd2
Name: dopamine receptor D2
Synonyms: D2R, Drd-2, D2 receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Transgenic (BAC)
NCBI: 13489
VEGA: 9
HGNC: HGNC:3023
Homologene: 22561
EGFP
Name: enhanced green fluorescent protein
Type: Gene
Species: Aequorea victoria (jellyfish)
Alteration at locus: Transgenic (BAC)
Tg(Drd2-EGFP)S118Gsat
Name: transgene insertion S118, GENSAT Project at Rockefeller University
Synonyms: Drd2-EGFP, D2GFP, BAC D2 EGFP, Founder Line S118, Tg(Drd2-EGFP)118Gsat
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic (BAC)
Genetic Alterations
Genotype modified to contain multiple copies of a modified BAC in which EGFP reporter gene is inserted immediately upstream of the coding sequence of the targeted gene. The BAC address for this line is RP23-161H15.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • Phenotype
    Site specific expression of green fluorescent protein. BAC-transgenic images of the central nervous system at various ages of this mouse can be found on the GENSAT database.
    MeSH Terms
    • Animals
    • Axons/metabolism
    • Cell Differentiation
    • Cell Lineage
    • Cell Movement
    • Central Nervous System/cytology
    • Central Nervous System/metabolism
    • Chromosomes, Artificial, Bacterial/genetics
    • Cyclic Nucleotide Phosphodiesterases, Type 1
    • Gene Expression Profiling/methods
    • Gene Expression Regulation, Developmental
    • Gene Library
    • Genes, Reporter/genetics
    • Genetic Vectors/genetics
    • Green Fluorescent Proteins
    • Homeodomain Proteins/genetics
    • Homeodomain Proteins/metabolism
    • LIM-Homeodomain Proteins
    • Luminescent Proteins/analysis
    • Luminescent Proteins/genetics
    • Membrane Glycoproteins/genetics
    • Membrane Glycoproteins/metabolism
    • Mice
    • Mice, Transgenic
    • Nerve Tissue Proteins/genetics
    • Nerve Tissue Proteins/metabolism
    • Neurosciences/methods
    • Phosphoric Diester Hydrolases/genetics
    • Phosphoric Diester Hydrolases/metabolism
    • Synapses/metabolism
    • Transcription Factors
    • Transgenes/genetics
    Strain Development
    Modified BAC containing inserted EGFP upstream of targeted gene injected into pronuclei of FVB/N fertilized oocytes. Hemizygous progeny mated to Swiss Webster mice each generation thereafter.
    Suggested Control Mice
    Wild-type littermates
    • Neurobiology
    • Developmental Biology
    • Cell Biology
    • Research Tools
    Donor
    Nathaniel Heintz, Ph.D., The Rockefeller University, GENSAT
    Primary Reference
    Gong S, Zheng C, Doughty ML, Losos K, Didkovsky N, Schambra UB, Nowak NJ, Joyner A, Leblanc G, Hatten ME, Heintz N. A gene expression atlas of the central nervous system based on bacterial artificial chromosomes. Nature. 2003 Oct 30;425(6961):917-25. (Medline PMID: 14586460)

    Colony and Husbandry Information

    In general, GENSAT BAC transgenic mouse lines should be utilized in the hemizygous state. Breeding of the BAC transgene to a homozygous state carries an increased risk of generating an unwanted phenotype for two reasons. First, the transgene inserts at random into the genome. Breeding the transgene to homozygosity results in mice that carry this insertion on both chromosomes, increasing the probability that the insertion will result in an observable phenotype. Second, BAC transgenes may carry passenger genes that are also expressed from the BAC vector. Breeding to homozygosity doubles the number of these genes carried in the transgenic mouse, increasing the possibility that a phenotype will result as a consequence of increased gene dosage. In our experience, observable phenotypes are rare in BAC transgenic mice when carried in the hemizygous state.
    Coat Color
    White
    MMRRC Breeding System
    Backcross, Sib-matting or Outcross to Swiss Webster
    Breeding Scheme(s)
    • Backcross:
      • Wild-type (C57BL/6J) female x Hemizygous Tg male or reciprocal mating
    • Sib-mating:
      • Wild-type female x Hemizygous Tg male littermate or reciprocal mating
    • Outcross:
      • Wild-type (Swiss Webster) female x Hemizygous Tg male littermate or reciprocal mating
    Generation
    N1F2
    NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Undetermined Undetermined
    Homozygotes are fertile: Undetermined Undetermined
    Hetero/Hemizygotes are fertile: Undetermined Yes
    Age Reproductive Decline: Undetermined Undetermined
    Average litter size
    8
    Recommended wean age
    3-4 weeks

    Order Request Information

    Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at mmrrc@med.unc.edu for more details.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    000230-UNC-HET-F
    000230-UNC-HET-M
    000230-UNC-WT-F
    000230-UNC-WT-M
    Heterozygous / Hemizygous Female
    Heterozygous / Hemizygous Male
    Wild Type Female
    Wild Type Male
    $285.00 / Non-Profit Per Mouse The mmrrc@med.unc.edu may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).
    000230-UNC-EMBRYO Cryo-preserved embryos $1,363.00 / Non-Profit Aliquot Approximate quantity3 : 20-40 embryos / aliquot
    000230-UNC-SPERM Cryo-preserved spermatozoa $548.00 / Non-Profit Aliquot Approximate quantity2

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.