Strain Name:
STOCK Ptf1atm1.1Mgn/Mmmh
Stock Number:
029175-MU
Citation ID:
RRID:MMRRC_029175-MU

Gene Information

Allele Symbol: Ptf1atm1.1Mgn (Mus musculus (mouse))
Name: pancreas specific transcription factor, 1a; targeted mutation 1.1, Mark A Magnuson
Synonyms: Ptf1a, Ptf1a, Ptf1a
Chromosome: 2
Alteration at locus: Knock-In
Monarch Initiative (Disease and Phenotype Associations): MGI:3691484
Gene Symbol: Ptf1a (Mus musculus (mouse))
Name: pancreas specific transcription factor, 1a
Synonyms: PTF1-p48, bHLHa29
Chromosome: 2
Alteration at locus: Knock-In
Monarch Initiative (Disease and Phenotype Associations): MGI:1328312
Genetic Alterations
This knock-in (null) allele was generated using Recombinase Mediated Cassette Exchange (RMCE) to insert a yellow fluorescent protein (YFP) replacement cassette into a Ptf1a loxed cassette acceptor allele. The Ptf1a[LFP] allele contains YPF coding sequences in exon 1 of Ptf1a. Exon 2 sequences were retained to supply RNA splicing and polyadenylation signal sequences. A loxP site is retained in the 5' flanking sequence as well as a single FRT site and an inverted loxP site in the 3' flanking sequence relative to the Ptf1a gene.
Genotype Determination
ES Cell Line
TL1/TL-1 derived from 129S6/SvEvTac
Phenotype
Homozygous: Homozygous mice are perinatal lethal. These mice are also characterized by agenesis of the pancreas and cerebellum as well as other cell specific defects in the central nervous system (CNS) and retina.

Heterozygous: Heterozygous animals are not known to exhibit any abnormal phenotype.
Mammalian Phenotype Terms
Allelic Composition: Ptf1atm1.1Mgn/Ptf1atm1.1Mgn (Genetic Background: Not Specified )

MeSH Terms
    Strain Development
    After achieving germline transmission, mice carrying the Ptf1a[YFP]allele with a hygromycin resistance (hygroR) cassette were bred to TgN(ACTFLPe)9205Dym animals in order to delete hygroR cassette. Ptf1a[YFP] mice lacking hygroR mice were then backcrossed to CD-1 outbred mice. The original line contained FRT flanked
    Suggested Control Mice
    • Wildtype littermates
    • Cancer
    • Cell Biology
    • Developmental Biology
    • Diabetes/Obesity
    • Endocrine Deficiency
    • Metabolism
    • Models for Human Disease
    • Neurobiology
    Donor
    Mark A. Magnuson, M.D., Vanderbilt University

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    029175-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
    Non-Profit / For-Profit
    Aliquot Approximate quantity2
    029175-MU-RESUS Litter recovered from cryo-archive $2,697.00 / $5,488.00
    Non-Profit / For-Profit
    Litter Recovered litter1; additional fees for any special requests.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.