Strain Name:
STOCK Ptf1atm2.1Mgn/Mmmh
Stock Number:
029175-MU
Citation ID:
RRID:MMRRC_029175-MU
Other Names:

STOCK Ptf1atm1.1Mgn/Mmmh

Gene Information

Ptf1atm2.1Mgn
Name: pancreas specific transcription factor, 1a; targeted mutation 2.1, Mark A Magnuson
Synonyms: Ptf1atm2Mgn, Ptf1aYFP, Ptf1aCitrine
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Knock-In
Ptf1a
Name: pancreas specific transcription factor, 1a
Synonyms: PTF1-p48, bHLHa29
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Knock-In
NCBI: 19213
Homologene: 10304
Genetic Alterations
This knock-in (null) allele was generated using Recombinase Mediated Cassette Exchange (RMCE) to insert a yellow fluorescent protein (YFP) replacement cassette into a Ptf1a-floxed cassette acceptor allele. The Ptf1a[LFP] allele contains YPF coding sequences in exon 1 of Ptf1a. Exon 2 sequences were retained to supply RNA splicing and polyadenylation signal sequences. A loxP site is retained in the 5' flanking sequence as well as a single FRT site and an inverted loxP site in the 3' flanking sequence relative to the Ptf1a gene.
Genotype Determination
ES Cell Line
TL1/TL-1 derived from 129S6/SvEvTac
Phenotype
Homozygous: Homozygous mice are perinatal lethal. These mice are also characterized by agenesis of the pancreas and cerebellum as well as other cell specific defects in the central nervous system (CNS) and retina.

Heterozygous: Heterozygous animals are not known to exhibit any abnormal phenotype.
Mammalian Phenotype Terms
Allelic Composition: Ptf1atm1.1Mgn/Ptf1atm1.1Mgn (Genetic Background: Not Specified )

MeSH Terms
  • Amacrine Cells/embryology
  • Amacrine Cells/metabolism
  • Animals
  • Cell Differentiation/genetics
  • Cell Differentiation/physiology
  • DNA Primers
  • Eye Proteins/metabolism
  • Forkhead Transcription Factors/metabolism
  • Gene Expression Regulation, Developmental
  • Immunohistochemistry
  • In Situ Hybridization
  • Mice
  • Retinal Horizontal Cells/embryology
  • Retinal Horizontal Cells/metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transcription Factors/metabolism
  • Transcription Factors/physiology
Strain Development

After achieving germline transmission, mice carrying the Ptf1a[YFP]allele with a hygromycin resistance (hygroR) cassette were bred to TgN(ACTFLPe)9205Dym animals (likely RRID:IMSR_JAX:005703) to delete the hygroR cassette. Ptf1a[YFP] mice lacking hygroR mice were then backcrossed to CD-1 outbred mice.

Suggested Control Mice

Wild-type littermates

  • Cancer
  • Cell Biology
  • Developmental Biology
  • Diabetes/Obesity
  • Endocrine Deficiency
  • Metabolism
  • Models for Human Disease
  • Neurobiology
Donor
Mark Magnuson, M.D., Vanderbilt University.
Primary Reference

Fujitani Y, Fujitani S, Luo H, Qiu F, Burlison J, Long Q, Kawaguchi Y, Edlund H, MacDonald RJ, Furukawa T, Fujikado T, Magnuson MA, Xiang M, Wright CV. Ptf1a determines horizontal and amacrine cell fates during mouse retinal development. Development. 2006 Nov;133(22):4439-50. doi: 10.1242/dev.02598. (Medline PMID: 17075007)

Additional References

Burlison JS, Long Q, Fujitani Y, Wright CV, Magnuson MA. Pdx-1 and Ptf1a concurrently determine fate specification of pancreatic multipotent progenitor cells. Dev Biol. 2008 Apr 1;316(1):74-86. doi: 10.1016/j.ydbio.2008.01.011. Epub 2008 Jan 26. (Medline PMID: 18294628)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color

Agouti or white

Eye

Black or pink

Breeding Scheme(s)

Per donor: "Male mice to CD-1 females every three months. Mating 12-20 week old Ptf1a[YFP] heterozygous"

Overall Breeding Performance

Excellent

Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown
Average litter size
12
Recommended wean age
3 weeks
Average Pups Weaned
12

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
029175-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
029175-MU-RESUS Litter recovered from cryo-archive $2,697.00 / $5,488.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.