Strain Name:
STOCK Onecut1tm1.1Mga/Mmnc
Stock Number:
029869-UNC
Citation ID:
RRID:MMRRC_029869-UNC
Other Names:
HNF6flox, Oc1flox

Gene Information

Onecut1tm1.1Mga
Name: one cut domain, family member 1; targeted mutation 1.1, Maureen A Gannon
Synonyms: Oc1flox, HNF6flox
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Targeted Mutation
Onecut1
Name: one cut domain, family member 1
Synonyms: Hnf6, Hfh12, HNF6, D9Ertd423e, OC-1, Oc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Targeted Mutation
NCBI: 15379
HGNC: HGNC:8138
Homologene: 3309
Genetic Alterations
A loxP-FRT Onecut1 (HNF6) [fl-neo] targeting vector was constructed with loxP sites flanking exon 1 containing the cut-domain, a neoR cassette located 5’ to the second loxP site and the neoR cassette was flanked by FRT sites. A herpes simplex virus-thymidine kinase cDNA was inserted outside the HNF6 gene homology region. The neo cassette was removed with a FLP deleter strain.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    TL1/TL-1
    Phenotype
    Homo-/Heterozygous: Same as wild-type
    MeSH Terms
    • Animals
    • Basic Helix-Loop-Helix Transcription Factors/metabolism
    • Body Patterning/genetics
    • Cell Differentiation
    • Cell Lineage
    • Cilia/metabolism
    • Cilia/pathology
    • Down-Regulation/genetics
    • Epithelium/embryology
    • Epithelium/metabolism
    • Epithelium/pathology
    • Gene Expression Regulation, Developmental
    • Gene Silencing
    • Gene Targeting
    • Hepatocyte Nuclear Factor 6/genetics
    • Hepatocyte Nuclear Factor 6/metabolism
    • Homeodomain Proteins/metabolism
    • Insulin-Secreting Cells/cytology
    • Insulin-Secreting Cells/metabolism
    • Islets of Langerhans/embryology
    • Islets of Langerhans/metabolism
    • Islets of Langerhans/pathology
    • Mice
    • Nerve Tissue Proteins/metabolism
    • Pancreatic Ducts/embryology
    • Pancreatic Ducts/metabolism
    • Pancreatic Ducts/pathology
    • Pancreatitis/metabolism
    • Pancreatitis/pathology
    • Stem Cells/cytology
    • Time Factors
    • Tumor Suppressor Proteins/metabolism
    Strain Development
    Onecut1-targeted mouse ES cells (TL1 on 129S6 background) were injected into mouse C57BL/6 blastocysts. High-percentage chimeras were bred with C57BL/6 wild-type mice to test germline transmission. Mice were sib-mated for 2-3 generations and the neoR cassette was removed in vivo by breeding of Onecut1flox-neo/+ animals with a Cre-transgenic line carrying the Tg(ACTFLPe)9205Dym allele (MGI:2448985) and ROSA26-transgenic mice for a few generations. Onecut1tm1.1Mga mice were backcrossed to C57BL/6 for 2-3 generations.
    Suggested Control Mice

    Wild-type littermates

    • Cancer
    • Cell Biology
    • Developmental Biology
    • Diabetes/Obesity
    • Endocrine Deficiency
    • Models for Human Disease
    • Neurobiology
    • Research Tools
    Donor
    Maureen A. Gannon, Ph.D., Vanderbilt University Medical Center.
    Primary Reference

    Zhang H, Ables ET, Pope CF, Washington MK, Hipkens S, Means AL, Path G, Seufert J, Costa RH, Leiter AB, Magnuson MA, Gannon M. Multiple, temporal-specific roles for HNF6 in pancreatic endocrine and ductal differentiation. Mech Dev. 2009 Dec;126(11-12):958-73. doi: 10.1016/j.mod.2009.09.006. Epub 2009 Sep 18. (Medline PMID: 19766716)

    Colony and Husbandry Information

    Onecut1[flox/w] mice were bred to B6D2 hybrid mice for line maintenance.

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu .
    Coat Color

    Black or agouti

    Eye
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    1 female X 1 male
    Overall Breeding Performance
    Unknown
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: unknown unknown
    Average litter size
    8
    Recommended wean age
    3 weeks
    Average Pups Weaned
    8

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    029869-UNC-EMBRYO Cryo-preserved embryos $1,363.00 / $2,263.00
    Non-Profit / For-Profit
    Aliquot Approximate quantity2 : 20-40 embryos / aliquot
    029869-UNC-SPERM Cryo-preserved spermatozoa $548.00 / $910.00
    Non-Profit / For-Profit
    Aliquot Approximate quantity3
    029869-UNC-RESUS Litter recovered from cryo-archive $2,829.00 / $4,696.00
    Non-Profit / For-Profit
    Litter Recovered litter4; additional fees for any special requests.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.