Strain Name:
B6.129S6-Mafatm1.1Rwst/Mmnc
Stock Number:
029994-UNC
Citation ID:
RRID:MMRRC_029994-UNC

Gene Information

Allele Symbol: Mafatm1.1Rwst [MGI:4457890] (Mus musculus (mouse))
Name: v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A (avian); targeted mutation 1.1, Roland W Stein
Synonyms: MafAfl, MafA[lox/w]
Chromosome: 15
Alteration at locus: Targeted Mutation
Gene Symbol: Mafa [MGI:2673307] (Mus musculus (mouse))
Name: v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A (avian)
Synonyms: RIPE3b1
Chromosome: 15
Alteration at locus: Targeted Mutation
Genetic Alterations
Two tandomly orientated loxP sites were introduced into the MafA gene locus flanking a 2355 bp fragment that contains: 1) 1106 bp upstream of the start codon 2) 1081 bp of the coding sequence 3)171 bp downstream of the stop codon.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    TL1/TL-1 derived from 129S6/SvEvTac
    Phenotype
    Homozygous: Beta cell dysfunction causing mild hyperglycemia

    Heterozygous: Beta cell dysfunction causing mild hyperglycemia, but not as severe as homozygous null animals
    Mammalian Phenotype Terms
    Allelic Composition: Mafatm1.1Rwst/Mafa+ (Genetic Background: involves: 129S6/SvEvTac )

    • phenotype not analyzed
    Strain Development
    After achieving germline transmission, mice carrying the targeted MafA(+neoR) allele were bred to TgN(ACTFLPe) 9205Dmy transgenic mice in order to delete the neoR cassette. MafA mice lacking neoR were then backcrossed to C57BL/6J inbred mice.
    Suggested Control Mice
    • Wildtype littermates
    • Developmental Biology
    • Diabetes/Obesity
    • Endocrine Deficiency
    • Metabolism
    Donor
    Roland W. Stein, Ph.D., Vanderbilt University

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu .

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    029994-UNC-EMBRYO Cryo-preserved embryos $1,363.00 / $2,263.00
    Non-Profit / For-Profit
    Aliquot Approximate quantity3 : 20-40 embryos / aliquot
    029994-UNC-RESUS Litter recovered from cryo-archive $2,829.00 / $4,696.00
    Non-Profit / For-Profit
    Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.