Homozygous phenotype: Hematopoietic cells express the CD45.1 antigen
Heterozygous phenotype: Hematopoietic cells express the CD45.1 antigen
Strain of Origin: SJL-> C57BL/6
Strain genetic background: DBA/2J
Strain Development: The SJL form of CD45 (CD45.1) was backcrossed on the B6 background a long time ago. Using the B6=CD45.1 mice, the CD45.1 gene was backcrossed on the D2 background. This created a CD45 congenic D2 mouse; the parental D2 mouse expresses the CD45.2 allele. The CD45 antigen is expressed on all cells of the hematopoietic system. The allelic forms of CD45 antigen are used to follow transplants of hematopoietic cells. This system is heavily used on the B6 background and we anticipate that the D2CD45.1 mice will be in high demand from the same scientific community.
Suggested Control Mice:
Donor: Christa Muller-Sieburg, Ph.D., Sidney Kimmel Cancer Center
Cho RH, Sieburg HB, Muller-Sieburg CE. A new mechanism for the aging of hematopoietic stem cells: aging changes the clonal composition of the stem cell compartment but not individual stem cells. Blood. 2008 Jun 15;111(12):5553-61. Epub 2008 Apr 15. (Medline PMID: 18413859 )
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
|Aliquot||Approximate quantity3: 20-40 embryos / aliquot|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.