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Strain Name:
D2.SJL(B6)-Ptprca/Mmucd
Stock Number:
030663-UCD
Citation ID:
RRID:MMRRC_030663-UCD
Other Names:
D2-CD45, D2-CD45.1, D2-CD45.1Cms, D2.SJL(B6)-Ptprca/Mmcd

Strain Information

Ptprca
Name: protein tyrosine phosphatase receptor type C; a variant
Synonyms: CD45.1, Ly5a, PtprcSJL
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Non-mutant
Ptprc
Name: protein tyrosine phosphatase receptor type C
Synonyms: T200, B220, CD45, Lyt-4, Ly-5
Type: Gene
Species: Mouse
Chromosome: 1
Alteration at locus: Non-mutant
NCBI: 19264
HGNC: HGNC:9666
Homologene: 2126
Phenotype
Homozygous: Hematopoietic cells express the CD45.1 antigen

Heterozygous: Hematopoietic cells express the CD45.1 antigen
MeSH Terms
  • Aging/physiology
  • Animals
  • B-Lymphocytes/cytology
  • B-Lymphocytes/physiology
  • Blood
  • Cell Lineage/physiology
  • Cellular Senescence/physiology
  • Clone Cells/cytology
  • Clone Cells/physiology
  • Genetic Markers
  • Hematopoietic Stem Cells/cytology
  • Hematopoietic Stem Cells/physiology
  • Lymphocytes/cytology
  • Lymphocytes/physiology
  • Mice
  • Mice, Congenic
  • Mice, Inbred Strains
  • Mice, Transgenic
  • Myeloid Cells/cytology
  • Myeloid Cells/physiology
  • T-Lymphocytes/cytology
  • T-Lymphocytes/physiology
  • Thymus Gland/cytology
Strain Development
The SJL form of CD45 (CD45.1) was backcrossed on the B6 background a long time ago. Using the B6=CD45.1 mice, the CD45.1 gene was backcrossed on the D2 background. This created a CD45 congenic D2 mouse; the parental D2 mouse expresses the CD45.2 allele. The CD45 antigen is expressed on all cells of the hematopoietic system. The allelic forms of CD45 antigen are used to follow transplants of hematopoietic cells. This system is heavily used on the B6 background and we anticipate that the D2CD45.1 mice will be in high demand from the same scientific community.
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Developmental Biology
  • Hematology
  • Immunology and Inflammation
  • Research Tools
Donor
Christa Muller-Sieburg, Ph.D., Sidney Kimmel Cancer Center
Primary Reference
Cho RH, Sieburg HB, Muller-Sieburg CE. A new mechanism for the aging of hematopoietic stem cells: aging changes the clonal composition of the stem cell compartment but not individual stem cells. Blood. 2008 Jun 15;111(12):5553-61. Epub 2008 Apr 15. (Medline PMID: 18413859)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
030663-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / $1,568.61
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
030663-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $882.81
Non-Profit / For-Profit
Aliquot Approximate quantity3
030663-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / $7,650.23
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.