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Strain Name:
STOCK Slit3tm1Dor Slit1tm1Matl/Mmmh
Stock Number:
030756-MU
Citation ID:
RRID:MMRRC_030756-MU

Strain Information

Slit1tm1Matl
Name: slit guidance ligand 1; targeted mutation 1, Marc Tessier-Lavigne
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Knockout
Slit3tm1Dor
Name: slit guidance ligand 3; targeted mutation 1, David M Ornitz
Synonyms: Slit3-
Type: Allele
Species: Mus musculus (mouse)
Chromosome:
Alteration at locus: Knockout
Slit1
Name: slit guidance ligand 1
Synonyms: Slil1
Type: Gene
Species: Mouse
Chromosome: 19
Alteration at locus: Knockout
NCBI: 20562
Homologene: 2302
Slit3
Name: slit guidance ligand 3
Synonyms: Slit1, b2b2362.1Clo
Type: Gene
Species: Mouse
Chromosome: 11
Alteration at locus: Knockout
NCBI: 20564
Homologene: 2303
Genetic Alterations
Slit1: IRES-tauGFP-loxpNeomycin inserted into N-terminal exon
Slit3: beta gal-loxpNeomycin inserted into exon 1
Genotype Determination
ES Cell Line
Slit1: D3
Slit3: CJ7
Phenotype
Homozygous: Diaphragmatic hernia. Axon guidance defects. Heart defects.
Heterozygous: Same as wild-type.
Mammalian Phenotype Terms
Allelic Composition: Slit1tm1Matl/Slit1+ (Genetic Background: involves: 129S2/SvPas )

Allelic Composition: Slit3tm1Dor/Slit3tm1Dor (Genetic Background: involves: 129S1/Sv * C57BL/6J )

MeSH Terms
  • Animals
  • Base Sequence
  • DNA Primers
  • Female
  • Hernia, Diaphragmatic/genetics
  • Hernias, Diaphragmatic, Congenital
  • Male
  • Membrane Proteins/genetics
  • Mice
  • Mice, Inbred C57BL
  • Models, Genetic
  • Mutagenesis
  • Axons/physiology
  • Diencephalon/embryology
  • Embryonic and Fetal Development/physiology
  • Intercellular Signaling Peptides and Proteins
  • Mice, Knockout/genetics
  • Nerve Tissue Proteins/deficiency
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/physiology
  • Neural Inhibition/physiology
  • Optic Chiasm/embryology
  • Preoptic Area/embryology
  • Retina/embryology
  • Retinal Ganglion Cells/physiology
  • Synaptic Transmission/physiology
  • Visual Pathways/embryology
Strain Development
Check publications. Includes breeding to CD-1 and C57BL/6.
Suggested Control Mice

Wild-type littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Cardiovascular
  • Cell Biology
  • Developmental Biology
  • Internal/Organ
  • Models for Human Disease
  • Neurobiology
  • Sensorineural
Donor
Marc Tessier-Lavigne, Ph.D., Stanford University.
David Ornitz, M.D., Washington University, School of Medicine.
Primary Reference

Yuan W; Rao Y; Babiuk RP; Greer JJ; Wu JY; Ornitz DM, A genetic model for a central (septum transversum) congenital diaphragmatic hernia in mice lacking Slit3., Proc Natl Acad Sci U S A 2003 Apr 29;100(9):5217-22 (Medline PMID: 12702769)

Plump AS; Erskine L; Sabatier C; Brose K; Epstein CJ; Goodman CS; Mason CA; Tessier-Lavigne M, Slit1 and Slit2 cooperate to prevent premature midline crossing of retinal axons in the mouse visual system., Neuron 2002 Jan 17;33(2):219-32 (Medline PMID: 11804570)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown
Bred to Homozygosity
Yes
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
030756-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
030756-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.