Strain Name:
C57BL/6J-Tg(Cd4-Il7r)1Wjl/Mmnc
Stock Number:
031041-UNC
Citation ID:
RRID:MMRRC_031041-UNC

Strain Information

Cd4
Name: CD4 antigen
Synonyms: Ly-4, L3T4
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12504
HGNC: HGNC:1678
Homologene: 513
Il7r
Name: interleukin 7 receptor
Synonyms: CD127, IL-7Ralpha, IL-7 receptor alpha chain
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 16197
VEGA: 15
HGNC: HGNC:6024
Homologene: 1646
Tg(Cd4-Il7r)1Wjl
Name: transgene insertion 1, Warren J Leonard
Synonyms: Tg IL-7Ralpha
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
To create IL-7R {alpha} transgenic mice, murine IL-7R{alpha} cDNA was amplified from mouse splenic T cells by RT-PCR, and the sequence was verified after cloning into pCR2.1 vector by TA cloning (Invitrogen). The cDNA was then subcloned into a CD4 enhancer/promoter transgenic vector, which lacks the CD4 silencer located in intron 1 and thus directs transgene expression in T lineage cells . The plasmid harboring the IL-7R{alpha} cDNA was then linearized and injected into C57BL/6 oocytes. The progenies were screened for the presence of the transgene by Southern blotting. Two positive mice were used as founders and bred on the C57BL/6 background.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • Phenotype
    Homozygous: Same as wild-type. 

    Hemizygous: Same as wild-type.
    MeSH Terms
    • Animals
    • CD4-Positive T-Lymphocytes/cytology
    • CD4-Positive T-Lymphocytes/immunology
    • CD4-Positive T-Lymphocytes/metabolism
    • CD8-Positive T-Lymphocytes/cytology
    • CD8-Positive T-Lymphocytes/immunology
    • CD8-Positive T-Lymphocytes/metabolism
    • Cell Death/genetics
    • Cell Death/immunology
    • Cell Proliferation
    • Cell Survival/genetics
    • Cell Survival/immunology
    • Epitopes, T-Lymphocyte/genetics
    • Epitopes, T-Lymphocyte/immunology
    • Growth Inhibitors/biosynthesis
    • Growth Inhibitors/genetics
    • Growth Inhibitors/physiology
    • Immunologic Memory/genetics
    • Listeria monocytogenes/immunology
    • Listeriosis/genetics
    • Listeriosis/immunology
    • Listeriosis/pathology
    • Lymphocyte Activation/genetics
    • Lymphocyte Count
    • Mice
    • Mice, Inbred C57BL
    • Mice, Transgenic
    • Receptors, Interleukin-7/biosynthesis
    • Receptors, Interleukin-7/genetics
    • Receptors, Interleukin-7/physiology
    Suggested Control Mice

    Wildtype littermates

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Immunology and Inflammation
    Donor
    Warren Leonard, Ph.D., National Institutes of Health/National Heart, Lung, and Blood Institute (NHLBI)
    Primary Reference
    Haring JS, Jing X, Bollenbacher-Reilley J, Xue HH, Leonard WJ, Harty JT. Constitutive expression of IL-7 receptor alpha does not support increased expansion or prevent contraction of antigen-specific CD4 or CD8 T cells following Listeria monocytogenes infection. J Immunol. 2008 Mar 1;180(5):2855-62. (Medline PMID: 18292507)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    031041-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.