Strain Detail Sheet

Strain Name    :

C57BL/6J-Tg(Cd4-Il7r)1Wjl/Mmnc

Stock Number :

031041-UNC

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Transgene: Tg(Cd4-Il7r)1Wjl
Name: transgene insertion 1, Warren J Leonard
Alteration at locus: Transgenic
Promoter: Cd4
Name: CD4 antigen
Alteration at locus: Transgenic
Transgene: Il7r
Name: interleukin 7 receptor
Alteration at locus: Transgenic

Genetic Alterations:
To create IL-7R {alpha} transgenic mice, murine IL-7R{alpha} cDNA was amplified from mouse splenic T cells by RT-PCR, and the sequence was verified after cloning into pCR2.1 vector by TA cloning (Invitrogen). The cDNA was then subcloned into a CD4 enhancer/promoter transgenic vector, which lacks the CD4 silencer located in intron 1 and thus directs transgene expression in T lineage cells . The plasmid harboring the IL-7R{alpha} cDNA was then linearized and injected into C57BL/6 oocytes. The progenies were screened for the presence of the transgene by Southern blotting. Two positive mice were used as founders and bred on the C57BL/6 background.

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: None

Hemizygous phenotype: None

Strain of Origin: C57BL/6J

Strain genetic background: C57BL/6J

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Immunology and Inflammation

Strain Origin

Donor: Warren Leonard, Ph.D., NIH/NHLBI

Primary Reference:

Haring JS, Jing X, Bollenbacher-Reilley J, Xue HH, Leonard WJ, Harty JT. Constitutive expression of IL-7 receptor alpha does not support increased expansion or prevent contraction of antigen-specific CD4 or CD8 T cells following Listeria monocytogenes infection. J Immunol. 2008 Mar 1;180(5):2855-62. (Medline PMID: 18292507)

Colony and Husbandry Information

Special Considerations

None.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
031041-UNC-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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Generated: 04/19/2014