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Strain Detail Sheet

Strain Name:
B6.129S6-Myctm2Fwa/Mmjax
Stock Number:
032046-JAX
Citation ID:
RRID:MMRRC_032046-JAX

Strain Information

Gene Details

Myctm2Fwa
Name: myelocytomatosis oncogene; targeted mutation 2, Frederick W Alt
Synonyms: Myclox, MYCfl, c-mycfl, Mycflox
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Targeted Mutation
Myc
Name: myelocytomatosis oncogene
Synonyms: c-myc, Nird, Niard, Myc2, bHLHe39
Type: Gene
Species: Mouse
Chromosome: 15
Alteration at locus: Targeted Mutation
NCBI: 17869
HGNC: HGNC:7553
Homologene: 31092

Additional Resources
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Google
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PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genotype Determination
ES Cell Line
TC1/TC-1 derived from 129S6/SvEvTac

Strain Description

Phenotype
Homozygous: Mice homozygous for the targeted mutation are viable and fertile. Homozygous mutant mice show no overt phenotypic abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. This mutant mouse strain may be useful in generating conditional mutations to study c-Myc in any organ/tissue, B cell activation and proliferation, neural crest cell development, and mouse embryonic fibroblast proliferation. For example, when crossed to a strain expressing Cre recombinase in B lymphoctye development, this mutant mouse strain may be useful in studies of B cell proliferation and activation. When crossed to a strain expressing Cre recombinase in the neural tube, this mutant mouse strain may be useful in studies of craniofacial development.

Heterozygous: Heterozygote phenotype is expected to be similar to homozygote phenotype.
Mammalian Phenotype Terms
Allelic Composition: Myctm2Fwa/Myctm2Fwa (Genetic Background: involves: 129S6/SvEvTac )
MeSH Terms
  • Animals
  • B-Lymphocytes/cytology
  • B-Lymphocytes/drug effects
  • B-Lymphocytes/metabolism
  • B-Lymphocytes/physiology
  • CD40 Antigens/immunology
  • Cell Cycle
  • Cell Cycle Proteins
  • Cells, Cultured
  • Cyclin-Dependent Kinase Inhibitor p27
  • Cyclin-Dependent Kinases/antagonists & inhibitors
  • Fibroblasts/cytology
  • Fibroblasts/metabolism
  • G1 Phase
  • Gene Targeting
  • Interleukin-4/immunology
  • Interleukin-4/pharmacology
  • Lymphocyte Activation
  • Mice
  • Mice, Inbred C57BL
  • Microtubule-Associated Proteins/metabolism
  • Mitogens/immunology
  • Mitogens/pharmacology
  • Mutation
  • Proto-Oncogene Proteins c-myc/genetics
  • Proto-Oncogene Proteins c-myc/metabolism
  • Proto-Oncogene Proteins c-myc/physiology
  • Resting Phase, Cell Cycle
  • Tumor Suppressor Proteins
  • Bromodeoxyuridine
  • Cell Proliferation
  • Cell Size
  • DNA Primers
  • Fluoresceins
  • Hepatocytes/metabolism
  • Immunohistochemistry
  • Liver/growth & development
  • Liver/metabolism
  • Mice, Knockout
  • Ploidies
  • Poly I-C
  • Polymerase Chain Reaction
  • Proto-Oncogene Proteins c-myc/deficiency
Strain Development
A targeting vector was designed to place a loxP site between exons 1 and 2 and a loxP -flanked PGKneo cassette downstream of exon 3. The construct was electroporated into 129S6/SvEvTac-derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The neomycin cassette was removed by transient transfection with a Cre recombinase expressing plasmid leaving loxP sites flanking exons 2 and 3. The resulting chimeric animals were crossed to C57BL/6 mice. The mice were then bred to mice on a mixed B6;129 background carrying the Mycntm1Psk allele. The Mycn allele was subsequently bred out of the line. The mice were then backcrossed to C57BL/6J for 6 generations before being transferred to the Repository. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
Suggested Control Mice
Wild-type littermates

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.

Strain Origin

Donor
Ignacio Moreno de Alboran, Ph.D., Universidad de Autonoma de Madrid
Primary Reference
  • de Alboran IM; O'Hagan RC; Gartner F; Malynn B; Davidson L; Rickert R; Rajewsky K; DePinho RA; Alt FW, Analysis of C-MYC function in normal cells via conditional gene-targeted mutation., Immunity 2001 Jan;14(1):45-55 (Medline PMID: 11163229)
  • Baena E; Gandarillas A; Vallespinos M; Zanet J; Bachs O; Redondo C; Fabregat I; Martinez-A C; de Alboran IM, c-Myc regulates cell size and ploidy but is not essential for postnatal proliferation in liver., Proc Natl Acad Sci U S A 2005 May 17;102(20):7286-91 (Medline PMID: 15857952)

Colony and Husbandry Information

Special Considerations

While maintaining a live colony, these mice are bred as homozygotes.

Health Status Report

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
032046-JAX-SPERM Cryo-preserved spermatozoa $459.00 / Non-Profit Aliquot Approximate quantity3
032046-JAX-RESUS Litter recovered from cryo-archive $2,123.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.