Strain Name:
B10.Cg-Tgmwe H3b Pax1un/ChwMmucd
Stock Number:
032651-UCD
Citation ID:
RRID:MMRRC_032651-UCD
Other Names:
B10.UW-D2Mit103-D2Mit492, B10.Cg-H3b we Pax1un /SnJMmucd

Strain Information

H3b
Name: histocompatibility 3b, Th stimulating
Synonyms: Hd-1, Hd1, Hd2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Pax1un
Name: paired box 1; undulated
Synonyms: un
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Spontaneous Mutation
Tgm3we
Name: transglutaminase 3, E polypeptide; wellhaarig
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Spontaneous Mutation
Pax1
Name: paired box 1
Synonyms: hbs, Pax-1, wavy tail, hunchback, wt
Type: Gene
Species: Mouse
Chromosome: 2
Alteration at locus: Spontaneous Mutation
NCBI: 18503
HGNC: HGNC:8615
Homologene: 4514
Tgm3
Name: transglutaminase 3, E polypeptide
Synonyms: we, TG E
Type: Gene
Species: Mouse
Chromosome: 2
Alteration at locus: Spontaneous Mutation
NCBI: 21818
Homologene: 20690
Genetic Alterations
Reduced congenic donor region replaces Tgmwe, H3b, Pax1un, and a alleles on C57BL/610SnJ background on chromosome 2. The region still includes Tgmwe, H3b, and Pax1un but excludes a.
Phenotype
Homozygous: Physical traits associated with selected recessive genotypes
Mammalian Phenotype Terms
Allelic Composition: Pax1un/Pax1un (Genetic Background: B6.Cg-Pax1un )

Allelic Composition: Pax1un/Pax1un (Genetic Background: Not Specified )

Allelic Composition: we/we (Genetic Background: Not Specified )

Allelic Composition: we/we3J (Genetic Background: involves 129P3/J * C57BL/10 * NZB/BlNJ * UW )

Allelic Composition: we/weBkr (Genetic Background: involves: 129 * C57BL/10 * UW )

MeSH Terms
  • Animals
  • Biometry
  • Chromosome Mapping
  • Female
  • Male
  • Mice/anatomy & histology
  • Mice/genetics
  • Mice, Congenic
  • Mice, Inbred Strains
  • Obesity/genetics
  • Phenotype
Strain Development
C57BL/10SnJ (B10) mice and B10.UW-H3b we Pax1un at/SnJ (UW) congenic mice were purchased from The Jackson Laboratory. The Jackson Laboratory website states the UW congenic was generation N8F84 when cryopreserved. UW mice were backcrossed to B10 mice. Resulting heterozygous N9 progeny exhibited dominant phenotypes: straight tail and black and tan coat. Several N9 progeny were bred to each other in an F2 intercross. The goal was to find progeny retaining the UW alleles for Pax1 (Pax1, wavy tail, recessive), but with B10 alleles for agouti (a, black, recessive to a). Progeny exhibiting this double recessive phenotype would be homozygous recessive for each gene since selection was done via visual examination for wavy tail (Pax1/Pax1) and all black coat (a/a), respectively. Assuming Mendelian inheritance, the expected ratio of double homozygous recessive progeny is not favorable and, indeed, donor never found F2 progeny with it despite producing dozens of litters. Donor then bred F2 progeny with all black coat (a/a) and straight tail (Pax1/Pax1, or Pax1/Pax1) to each other in an F3 intercross in order to increase the percent chance of obtaining the double recessive phenotype. After several litters from several breeding pairs, 2 males and 1 female sibling with wavy tail and all black coat were finally produced. The colony donated to the MMRRC F27) descended from these 3 sibling founders and maintained via random intra-strain mating. As it happened, the recessive wellhaarig gene (we) was also retained so the mice also exhibit wavy hair/curly whiskers and are, therefore, actually triple homozygous recessive.
Suggested Control Mice

Wild-type littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Developmental Biology
  • Diabetes/Obesity
  • Immunology and Inflammation
  • Metabolism
  • Models for Human Disease
Donor
Craig Warden, Ph.D., University of California Davis
Primary Reference
Warden CH; Stone S; Chiu S; Diament AL; Corva P; Shattuck D; Riley R; Hunt SC; Easlick J; Fisler JS; Medrano JF, Identification of a congenic mouse line with obesity and body length phenotypes., Mamm Genome 2004 Jun;15(6):460-71. (Medline PMID: 15181538)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
032651-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / $1,545.65
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
032651-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $869.88
Non-Profit / For-Profit
Aliquot Approximate quantity3
032651-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / $7,650.23
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.