Strain Name:
C57BL/6J-Tlr9M7Btlr/Mmjax
Stock Number:
034329-JAX
Citation ID:
RRID:MMRRC_034329-JAX
Major Collection:

Gene Information

Tlr9M7Btlr
Name: toll-like receptor 9; mutation 7, Bruce Beutler
Synonyms: CpG11
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
Tlr9
Name: toll-like receptor 9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 81897
Homologene: 68126
Genetic Alterations
This mutation, discovered in a screen of progeny of ethylnitrosourea (ENU) mutagenized mice for impaired responses to Toll-like receptor (TLR) ligands, has been identified as a T to C transition at nucleotide position 1181 of the transcript that is predicted to result in the replacement of serine by proline at amino acid position 359 of the protein (S359P), in the second leucine rich repeat.
Genotype Determination
Phenotype
Homozygous: Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. In response to stimulation with oligodeoxynucleotides containing CpG motifs, macrophages do not produce tumor necrosis factor (TNF) alpha. This mutant mouse strain may be useful in studies of the role of toll like receptor 9 (TLR9) in the immune system.

Heterozygous: Macrophages from heterozygous mice produce a reduced level of TNF-alpha in response to CpG stimulation.
Mammalian Phenotype Terms
Allelic Composition: Tlr9M7Btlr/Tlr9M7Btlr (Genetic Background: C57BL/6J-Tlr9M7Btlr )

  • immune system
    • decreased tumor necrosis factor secretion [MP:0008561]
Allelic Composition: Tlr9M7Btlr/Tlr9+ (Genetic Background: C57BL/6J-Tlr9M7Btlr )

  • immune system
    • decreased tumor necrosis factor secretion [MP:0008561]
Strain Development
This missense point mutation was generated by ethylnitrosourea (ENU) mutagenesis in C57BL/6J males. Mutagenized males were outcrossed to C57BL/6J females. The mutation results in a T to C transition at position 1181 of the Tlr9 transcript. The mutation alters the corresponding amino acid from serine to proline at position 359 (S359P) in the eleventh leucine rich repeat. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
Suggested Control Mice
Wild-type littermates
  • Immunology and Inflammation
Donor
Bruce Beutler, Ph.D., The Scripps Research Institute
Primary Reference
Hua Huang and Bruce Beutler. \"CpG11 is an allele of Tlr9 and causes Tlr9 signaling defects\". Record for CpG11. MUTAGENETIX (TM), B. Beutler and colleagues, Department of Genetics, The Scripps Research Institute, La Jolla, CA.

Colony and Husbandry Information

While maintaining a live colony, these mice are bred as homozygotes.

Colony\'s Current Health Status Report

For more information about this colony's health status contact csmmrrc@jax.org

Order Request Information

Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at csmmrrc@jax.org for more details.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
034329-JAX-HOM-F
034329-JAX-HOM-M
Homozygous Female
Homozygous Male
$218.00 / Non-Profit Per Mouse The csmmrrc@jax.org may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).
034329-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.