Strain Name:
STOCK Sox17tm2(EGFP/cre)Mgn/Mmnc
Stock Number:
Citation ID:
Other Names:
Sox17tm1.3(Cre.GFP)Mgn, Sox17(Cre/GFP), Sox17.Cre.GFP

Strain Information

Name: SRY (sex determining region Y)-box 17; targeted mutation 2, Mark A Magnuson
Synonyms: Sox17.Cre-GFP, Sox17GFPCre
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Targeted Mutation
Name: SRY (sex determining region Y)-box 17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Targeted Mutation
NCBI: 20671
Homologene: 7948
Genetic Alterations
Using an recombinase-mediated cassette exchange (RMCE) strategy, a Cre-GFP (Green Fluorescent Protein) fusion gene was inserted into a Sox17[LCA] allele thereby replacing Sox17 coding sequences.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    TL1/TL-1 (129S6/SvEvTac)

    Homozygous: Not characterized.

    Hemizygous: These mice will mark the expression of Sox17 with both Cre and GFP. Cre will enable lineage tracing using reporter alleles that are activated by cre recombination. GFP will enable direct visualization of Sox17 gene expression.

    Mammalian Phenotype Terms
    Allelic Composition: Sox17tm2(EGFP/cre)Mgn/Sox17+ (Genetic Background: involves: 129S6/SvEvTac * CD-1 )

    MeSH Terms
    • Animals
    • Cell Differentiation
    • Cell Lineage
    • Embryo, Mammalian
    • Embryonic Development
    • Endoderm/cytology
    • Endoderm/metabolism
    • Fetal Stem Cells/cytology
    • Fetal Stem Cells/metabolism
    • Flow Cytometry
    • Gene Expression Regulation, Developmental
    • Green Fluorescent Proteins/genetics
    • HMGB Proteins/genetics
    • HMGB Proteins/metabolism
    • Hematopoietic Stem Cells/cytology
    • Hematopoietic Stem Cells/metabolism
    • Mice
    • Mice, Transgenic
    • RNA, Messenger/biosynthesis
    • SOXF Transcription Factors/genetics
    • SOXF Transcription Factors/metabolism
    Strain Development
    Chimeric mice containing the Sox17GFPCre allele were derived by the microinjection of clone 1G3:1C10 ESCs into blastocysts of C57BL/6J mice. After germline transmission, the HygroR cassette was removed by cross-breeding with ACTB:FLPe mice. The Sox17GFPCre allele was maintained within a CD1 background.
    Suggested Control Mice
    Wild-type littermates
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact Older strains may not have this information.
    • Diabetes/Obesity
    • Internal Organ
    • Research Tools
    Mark Magnuson, M.D., Vanderbilt University.
    Primary Reference
    Choi E, Kraus MR, Lemaire LA, Yoshimoto M, Vemula S, Potter LA, Manduchi E, Stoeckert CJ Jr, Grapin-Botton A, Magnuson MA. Dual lineage-specific expression of Sox17 during mouse embryogenesis. Stem Cells. 2012 Oct;30(10):2297-308. (Medline PMID: 22865702)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email .
    Coat Color
    MMRRC Breeding System
    Breeding Scheme(s)
    • CD1 female x Hemizygous male or reciprocal mating
    Overall Breeding Performance
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Undetermined Undetermined
    Average litter size
    Recommended wean age
    3-4 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    036463-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to for more information.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.