Strain Name:
STOCK Sox17tm2(EGFP/cre)Mgn/Mmnc
Stock Number:
036463-UNC
Citation ID:
RRID:MMRRC_036463-UNC
Other Names:
Sox17tm1.3(Cre.GFP)Mgn, Sox17(Cre/GFP), Sox17.Cre.GFP

Gene Information

Allele Symbol: Sox17tm2(EGFP/cre)Mgn (Mus musculus (mouse))
Name: SRY (sex determining region Y)-box 17; targeted mutation 2, Mark A Magnuson
Synonyms: Sox17.Cre-GFP, Sox17
Chromosome: 1
Alteration at locus: Targeted Mutation
Monarch Initiative (Disease and Phenotype Associations): MGI:5468103
Gene Symbol: Sox17 (Mus musculus (mouse))
Name: SRY (sex determining region Y)-box 17
Chromosome: 1
Alteration at locus: Targeted Mutation
Monarch Initiative (Disease and Phenotype Associations): MGI:107543
Genetic Alterations
Using an recombinase-mediated cassette exchange (RMCE) strategy, a Cre-GFP (Green Fluorescent Protein) fusion gene was inserted into a Sox17[LCA] allele thereby replacing Sox17 coding sequences.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    TL1/TL-1 derived from 129S6/SvEvTac
    Phenotype

    Homozygous: Not characterized.

    Hemizygous: These mice will mark the expression of Sox17 with both Cre and GFP. Cre will enable lineage tracing using reporter alleles that are activated by cre recombination. GFP will enable direct visualization of Sox17 gene expression.

    Mammalian Phenotype Terms
    Allelic Composition: Sox17tm2(EGFP/cre)Mgn/Sox17+ (Genetic Background: involves: 129S6/SvEvTac * CD-1 )

    MeSH Terms
    • Animals
    • Cell Differentiation
    • Cell Lineage
    • Embryo, Mammalian
    • Embryonic Development
    • Endoderm/cytology
    • Endoderm/metabolism
    • Fetal Stem Cells/cytology
    • Fetal Stem Cells/metabolism
    • Flow Cytometry
    • Gene Expression Regulation, Developmental
    • Green Fluorescent Proteins/genetics
    • HMGB Proteins/genetics
    • HMGB Proteins/metabolism
    • Hematopoietic Stem Cells/cytology
    • Hematopoietic Stem Cells/metabolism
    • Mice
    • Mice, Transgenic
    • RNA, Messenger/biosynthesis
    • SOXF Transcription Factors/genetics
    • SOXF Transcription Factors/metabolism
    Strain Development
    Chimeric mice containing the Sox17GFPCre allele were derived by the microinjection of clone 1G3:1C10 ESCs into blastocysts of C57BL/6J mice. After germline transmission, the HygroR cassette was removed by cross-breeding with ACTB:FLPe mice. The Sox17GFPCre allele was maintained within a CD1 background.
    Suggested Control Mice
    Wild-type littermates
    • Diabetes/Obesity
    • Internal Organ
    • Research Tools
    Donor
    Mark Magnuson, Vanderbilt University.
    Primary Reference
    Choi E, Kraus MR, Lemaire LA, Yoshimoto M, Vemula S, Potter LA, Manduchi E, Stoeckert CJ Jr, Grapin-Botton A, Magnuson MA. Dual lineage-specific expression of Sox17 during mouse embryogenesis. Stem Cells. 2012 Oct;30(10):2297-308. (Medline PMID: 22865702)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu .
    Coat Color
    White
    MMRRC Breeding System
    Backcross
    Breeding Scheme(s)
    • CD1 female x Hemizygous male or reciprocal mating
    Generation
    N4
    Overall Breeding Performance
    Excellent
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Undetermined Undetermined
    Average litter size
    Undetermined
    Recommended wean age
    3-4 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    036463-UNC-RESUS Litter recovered from cryo-archive $2,829.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.