Strain Name:
B6.Cg-Tg(Thy1-MAPT*)2652Gds/Mmmh
Stock Number:
036717-MU
Citation ID:
RRID:MMRRC_036717-MU
Other Names:
Tg2652, Tau4RTg2652

Gene Information

Thy1
Name: thymus cell antigen 1, theta
Synonyms: Thy1.1, Thy 1.2, CD90, Thy1.2, Thy-1, T25, Thy-1.2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Transgenic
NCBI: 21838
Homologene: 4580
MAPT
Name: microtubule-associated protein tau
Type: Gene
Species: Homo sapiens (human)
Chromosome: 17
Alteration at locus: Transgenic
Tg(Prnp-MAPT*)2652Gds
Name: transgene insertion 2652, Gerard Schellenberg
Synonyms: Tg(pThy1.2-MAPT*)2652Gsd, Tau4RTg2652, Tg2652, Tg(Thy1-MAPT*)2652Gds
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
This transgenic mouse contains the cDNA of human MAPT (microtubule associated protein, tau) which encodes the 1N4R brain isoform of tau under the control of a mouse neuron-specific Thy1 promoter.
Genotype Determination
Phenotype

Homozygous: Mice are viable but suspected non-fertile. Homozygous mice are smaller than the hemizygous mice and have more pronounced muscle atrophy. Neuropathology is similar to that seen in hemizygous mice.

Hemizygous: Central nervous system abnormalities: human tau is expressed in the brain and spinal cord. These mice have a motor phenotype characterized by an abnormal gait, muscle weakness, poor rotarod performance. Muscle atrophy is also seen in the limbs. Immunohistochemical examination of the brain and spinal cord of these mice show robust tau pathology in the form of somatodendritic accumulation of hyperphosphorylated human tau (AT8 immunoreactivity) and conformationally altered tau (MC1 immunoreactivity) throughout the brain. Dystrophic neurites are also immunoreactive with AT8 and MC1. No silver positive (Gallyas silver stain) neurofibrillary tangles were seen in mice aged to 1.5 - 2yrs years.

Strain Development
The transgene was injected into a B6C3 zygote and backcrossed to C57BL/6J. The transgenic mice were continually backcrossed to C57BL/6J for 7 generations.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
  • Models for Human Disease
    • Alzheimer's Disease
  • Neurobiology
  • Sensorineural
Donor
Gerard Schellenberg, Ph.D., Perelman School of Medicine at the University of Pennsylvania
Primary Reference
Wheeler JM, McMillan PJ, Hawk M, Iba M, Robinson L, Xu GJ4, Dombroski BA, Jeong D, Dichter MA, Juul H, Loomis E, Raskind M, Leverenz JB, Trojanowski JQ, Lee VM, Schellenberg GD, Kraemer BC. High copy wildtype human 1N4R tau expression promotes early pathological tauopathy accompanied by cognitive deficits without progressive neurofibrillary degeneration. Acta Neuropathol Commun. 2015 Jun 4;3:33. (Medline PMID: 26041339).

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
Other
Muscle atrophy of the limbs, abnormal gait, whole body "twitching", slight.
MMRRC Breeding System
Backcross
Breeding Scheme(s)

Wild-type (C57BL/6J) female x Hemizygous male or reciprocal mating

Generation
N7+
Overall Breeding Performance
Not specified
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Undetermined Undetermined suspected non-fertile
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
4-6
Recommended wean age
3-4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
036717-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity2
036717-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.