Strain Name:
B6.FVB(Cg)-Tg(Nr4a1-EGFP)GY139Gsat/WeisMmucd
Stock Number:
036737-UCD
Citation ID:
RRID:MMRRC_036737-UCD
Other Names:
originally from MMRRC : 012015-UCD, STOCK Tg(Nr4a1-EGFP)GY139Gsat/Mmucd, Nur77-GFP

Gene Information

EGFP
Name: enhanced green fluorescent protein
Type: Gene
Species: Aequorea victoria (jellyfish)
Alteration at locus: Transgenic (BAC)
Nr4a1
Name: nuclear receptor subfamily 4, group A, member 1
Synonyms: Gfrp, TR3, NP10, Hmr, GFRP1, NGFI-B, Nur77, TIS1, N10, Hbr1, Hbr-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Transgenic (BAC)
NCBI: 15370
VEGA: 15
HGNC: HGNC:7980
Homologene: 1612
Tg(Nr4a1-EGFP)GY139Gsat
Name: transgene insertion GY139, GENSAT Project at Rockefeller University
Synonyms: Nur77-eGFP BAC
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic (BAC)
Genetic Alterations
eGFP was inserted at the start site of the Nur77 gene (Nr4a1) in a BAC transgene. eGFP is expressed under the control of the Nur77 regulatory region.
Phenotype

Homozygous: Not evaluated

Hemizygous: Animals with this transgene express GFP in hematopoietic cells. Specifically, TCR and BCR stimulation induces upregulation of GFP both in vivo and in vitro.

MeSH Terms
  • Animals
  • Antigens/immunology
  • Autoantibodies/immunology
  • Autoantigens/immunology
  • Autoimmune Diseases/immunology
  • Autoimmune Diseases/pathology
  • B-Lymphocytes/cytology
  • B-Lymphocytes/immunology
  • Calcium/metabolism
  • Calcium Signaling
  • Clonal Anergy/immunology
  • Down-Regulation
  • Genes, Reporter
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Immune Tolerance/immunology
  • Immunoglobulin D/immunology
  • Immunoglobulin M/immunology
  • Lymphocyte Activation/immunology
  • Mice
  • Models, Immunological
  • Nuclear Receptor Subfamily 4, Group A, Member 1/genetics
  • Receptors, Antigen, B-Cell/immunology
  • Receptors, Antigen, B-Cell/metabolism
  • Signal Transduction/immunology
  • Spleen/cytology
  • Spleen/immunology
  • T-Lymphocytes/cytology
  • T-Lymphocytes/immunology
  • Transgenes/genetics
Strain Development
The original transgenic mice were created in the GENSAT project. The transgenic mice have a modified BAC-EGFP injected into pronuclei of FVB/N fertilized oocytes. Offspring of founders were crossed to Swiss Webster mice and donated to the MMRRC. Dr. Weiss lab obtained the STOCK from MMRRC, 012015-UCD, STOCK Tg(Nr4a1-EGFP)GY139Gsat/Mmucd, and backcrossed it to C57BL/6. Expression patterns have not been confirmed in this backcrossed line. The publication for this strain indicates that there were segregating copies of the transgene on different loci, resulting in HI and LO sub-lines. This backcrossed line received at the MMRRC is stated to be the HI sub-line that was noted in the publication.
Suggested Control Mice
Wild-type littermates or C57BL/6
  • Immunology and Inflammation
Donor

Arthur Weiss, M.D., Ph.D., University of California, San Francisco; Original strain was donated to MMRRC by Nathaniel Heintz, Ph.D., The Rockefeller University, GENSAT

Primary Reference

Zikherman J, Parameswaran R, Weiss A. Endogenous antigen tunes the responsiveness of naive B cells but not T cells. Nature. 2012 Sep 6;489(7414):160-4. (Medline PMID: 22902503).

Colony and Husbandry Information

Hemizygous transgenics are outcrossed to wild-type C57BL/6 mates.

In general, GENSAT BAC transgenic mouse lines should be utilized in the hemizygous state. Breeding of the BAC transgene to a homozygous state carries an increased risk of generating an unwanted phenotype for two reasons. First, the transgene inserts at random into the genome. Breeding the transgene to homozygosity results in mice that carry this insertion on both chromosomes, increasing the probability that the insertion will result in an observable phenotype. Second, BAC transgenes may carry passenger genes that are also expressed from the BAC vector. Breeding to homozygosity doubles the number of these genes carried in the transgenic mouse, increasing the possibility that a phenotype will result as a consequence of increased gene dosage. In our experience, observable phenotypes are rare in BAC transgenic mice when carried in the hemizygous state.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu .
Coat Color
Black
MMRRC Breeding System
Backcross to C57BL/6
Breeding Scheme(s)

C57BL/6 female x Hemizygous male or reciprocal mating.

Generation
N8
Overall Breeding Performance
Good
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Unknown Unknown
Homozygotes are fertile: Unknown Unknown
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown
Average litter size
5-9
Recommended wean age
3-4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
036737-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity2
036737-UCD-RESUS Litter recovered from cryo-archive $3,033.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.