Strain Name:
B6.129S6(SJL)-Gt(ROSA)26Sortm2.1(mix1b-mCherry)Mgn/Mmjax
Stock Number:
036911-JAX
Citation ID:
RRID:MMRRC_036911-JAX
Other Names:
Rosa26{(228.AR8.TA.mCherry)Hri}, Rosa26-228.AR8.mCherry

Gene Information

Allele Symbol: Gt(ROSA)26Sortm2.1(mix1b-mCherry)Mgn (Mus musculus (mouse))
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2.1, Mark A Magnussen
Synonyms: -228/AR8-TA-mCherry, Rosa26<(228.AR8.TA.mCherry)Hri>
Chromosome: 6
Alteration at locus: Knock-In
Monarch Initiative (Disease and Phenotype Associations): MGI:5476468
Reporter: mRFP (Discosoma sp. (mushroom coral))
Name: monomeric red fluorescent protein
Alteration at locus: Knock-In
Monarch Initiative (Disease and Phenotype Associations):
Gene Symbol: mix1-b (Xenopus laevis (African clawed frog))
Name: Mix paired-like homeobox
Alteration at locus: Knock-In
Monarch Initiative (Disease and Phenotype Associations):
Genetic Alterations
The pRosa26-228 AR8 TA-Cherry vector was made using a basal exchange vector which contains a 5.166 kb sequence from the ROSA26 locus, Lox71/Lox2272 sites, and a FRT site (FLP flanked Pgk-hygR gene) cassette that is used for positive selection of ES cells after RMCE. An upstream FLP flanked hygromycin selection cassette was subsequently removed by flp recombinase. Eventually, the Rosa26 gene sequence (-228 to +81) was replaced by the Foxh1/Smad2-dependent, Mix2-derived Activin response element (AR8) fused to a TATA-mCherry reporter.
ES Cell Line
TL1/TL-1 derived from 129S6/SvEvTac
Phenotype

Homozygous: not characterized

Heterozygous: healthy and normal phenotype

Mammalian Phenotype Terms
Allelic Composition: Gt(ROSA)26Sortm2.1(mix1b-mCherry)Mgn/Gt(ROSA)26Sortm2.1(mix1b-mCherry)Mgn (Genetic Background: involves: 129S6/SvEvBrd * C57BL/6J * SJL )

Strain Development
TL1 ES Cells containing a targeted vector were microinjected into blastocysts of C57BL/6J mice. The resulting chimeric male offsprings were bred with C57BL/6J female mice to acheive germline transmission. Then, these mice were crossed with ACTB:FLPe mice to remove the hygromycin resistant gene and then backcrossed for 7 generations with C57BL/6J.
Suggested Control Mice
Wild-type littermates
  • Cancer
  • Cardiovascular
  • Cell Biology
  • Dermatology
  • Developmental Biology
  • Diabetes
  • Endocrine Deficiency
  • Hematology
  • Immunology and Inflammation
  • Internal Organ
  • Metabolism
  • Models for Human Disease
  • Neurobiology
  • Obesity
  • Reproduction
Donor
Mark Magnuson, Vanderbilt University.
Primary Reference

Serup P, Gustavsen C, Klein T, Potter LA, Lin R, Mullapudi N, Wandzioch E, Hines A, Davis A, Bruun C, Engberg N, Petersen DR, Peterslund JM, Macdonald RJ, Grapin-Botton A, Magnuson MA, Zaret KS. Partial promoter substitutions generating transcriptional sentinels of diverse signaling pathways in embryonic stem cells and mice. Dis Model Mech. 2012 Nov;5(6):956-66. Epub 2012 Aug 10. (Medline PMID: 22888097).

Colony and Husbandry Informationon

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Coat Color
Black
MMRRC Breeding System
Backcross
Breeding Scheme(s)
  • Wild-type (C57BL/6J) female x Heterozygous male or reciprocal mating
Generation
N7
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Unknown Unknown
Homozygotes are fertile: Unknown Unknown
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 6 months 6 months
Average litter size
5-9
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
036911-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity2
036911-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.