Loading Mouse GIF
Loading...

Strain Name:
B6.129S6(SJL)-Gt(ROSA)26Sortm2.1(mix1b-mCherry)Mgn/Mmjax
Stock Number:
036911-JAX
Citation ID:
RRID:MMRRC_036911-JAX
Other Names:
Rosa26{(228.AR8.TA.mCherry)Hri}, Rosa26-228.AR8.mCherry

Strain Information

Gt(ROSA)26Sortm2.1(mix1b-mCherry)Mgn
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2.1, Mark A Magnussen
Synonyms: Rosa26(228.AR8.TA.mCherry)Hri, -228/AR8-TA-mCherry
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Knock-In
mRFP
Name: monomeric red fluorescent protein
Type: DNA Segment
Species: Discosoma sp. (mushroom coral)
Chromosome:
Alteration at locus: Knock-In
Genetic Alterations

Mice harboring the pR26-228 AR8-TA-mCherry reporter express mCherry in cells and tissues that receive SMAD2/3 mediated signaling induced by Nodal/Activin. This strain allows live monitoring of SMAD2/3-mediated signaling events seen in embryonic development as well as during regeneration and homeostasis in adults.

ES Cell Line
TL1
Phenotype

Mice harboring the pR26-228 AR8-TA-mCherry reporter are viable and fertile, with expression of mCherry under the control of the activin response element from the Xenopus laevis mix1b (Mix.2) promoter. The red fluorescent protein, mCherry, is expressed in cells and tissues that receive SMAD2/3 mediated signaling induced by Nodal/Activin. The Nodal signal transduction pathway is involved in mesoendoderm induction, patterning of the nervous system, and determination of dorsal- ventral axis in vertebrate embryos. This strain allows live monitoring of SMAD2/3-mediated signaling events seen in particular lineages and stages in embryonic development as well as during regeneration and homeostasis in adults. Additionally, it allows FACS-based isolation of responding cells.

Mammalian Phenotype Terms
Allelic Composition: Gt(ROSA)26Sortm2.1(mix1b-mCherry)Mgn/Gt(ROSA)26Sortm2.1(mix1b-mCherry)Mgn (Genetic Background: involves: 129S6/SvEvBrd * C57BL/6J * SJL )

MeSH Terms
  • Activins/genetics
  • Activins/metabolism
  • Animals
  • Base Sequence
  • Bone Morphogenetic Proteins/genetics
  • Bone Morphogenetic Proteins/metabolism
  • Embryo, Mammalian/drug effects
  • Embryo, Mammalian/metabolism
  • Embryonic Stem Cells/drug effects
  • Embryonic Stem Cells/metabolism
  • Genetic Engineering
  • Genetic Loci/genetics
  • Humans
  • Mice
  • Molecular Sequence Data
  • Mutation/genetics
  • Promoter Regions, Genetic
  • Proteins/genetics
  • RNA, Untranslated
  • Rats
  • Receptors, Notch/genetics
  • Receptors, Notch/metabolism
  • Recombination, Genetic/genetics
  • Response Elements/genetics
  • Sequence Deletion/genetics
  • Signal Transduction/drug effects
  • Signal Transduction/genetics
  • Transcription, Genetic/drug effects
  • Tretinoin/pharmacology
  • Wnt Signaling Pathway/drug effects
  • Wnt Signaling Pathway/genetics
Strain Development
Using the ROSA26LCA or Loxed Cassette Acceptor allele (Gt(ROSA)26Sortm1Mgn), which contains a loxP-flanked (lox71, lox2272) selection cassette in the ROSA26 promoter, a donor cassette is inserted into the acceptor allele via recombinase mediated cassette exchange (RMCE). The donor cassette or signaling sentinel construct includes a frt-flanked pgk-hygromycin and an activin response element (AR8) from the Xenopus laevis mix1b reporter fused to a TATA-mCherry reporter, which replaced the ROSA26 promoter sequence (-4 kb to -228 bp). The construct was electroporated into 129S6/SvEvTac-derived TL1 ES cells. Correctly targeted ES cells were injected into blastocysts from C57BL/6J mice. Offspring were bred with B6;SJL-Tg(ACTFLPe)9205Dym/J transgenic mice to delete the pgk-hygromycin cassette. The donating investigator reported that the mice were backcrossed to C57BL/6J mice for 7 generations prior to sending to MMRRC at JAX.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Cancer
  • Cardiovascular
  • Cell Biology
  • Dermatology
  • Developmental Biology
  • Diabetes
  • Endocrine Deficiency
  • Hematology
  • Immunology and Inflammation
  • Internal Organ
  • Metabolism
  • Models for Human Disease
  • Neurobiology
  • Obesity
  • Reproduction
Donor
Mark Magnuson, M.D., Vanderbilt University.
Primary Reference

Serup P, Gustavsen C, Klein T, Potter LA, Lin R, Mullapudi N, Wandzioch E, Hines A, Davis A, Bruun C, Engberg N, Petersen DR, Peterslund JM, Macdonald RJ, Grapin-Botton A, Magnuson MA, Zaret KS. Partial promoter substitutions generating transcriptional sentinels of diverse signaling pathways in embryonic stem cells and mice. Dis Model Mech. 2012 Nov;5(6):956-66. Epub 2012 Aug 10. (Medline PMID: 22888097).

Colony and Husbandry Information

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.
Coat Color
Black
MMRRC Breeding System
Backcross
Breeding Scheme(s)
  • Wild-type (C57BL/6J) female x Heterozygous male or reciprocal mating
Generation
N7
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Unknown Unknown
Homozygotes are fertile: Unknown Unknown
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 6 months 6 months
Average litter size
5-9
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
036911-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
036911-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.