Strain Name:
C57BL/6J-Cdh23m1Btlr/Mmmh
Stock Number:
036997-MU
Citation ID:
RRID:MMRRC_036997-MU
Other Names:
dee dee
Major Collection:

Gene Information

Cdh23m1Btlr
Name: cadherin 23 (otocadherin); mutation 1, Bruce Beutler
Synonyms: dee dee
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
Cdh23
Name: cadherin 23 (otocadherin)
Synonyms: USH1D, mdfw, ahl, nmf112, nmf181, nmf252, 4930542A03Rik, sals, bob
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 22295
Homologene: 11142
Genetic Alterations
The dee dee mutation is a G to T transversion at base pair 60308056 (v38) on Chromosome 10, equivalent to base pair 388435 in the GenBank genomic region NC_000076 encoding Cdh23. The mutation corresponds to residue 9176 in the NM_023370.3 mRNA sequence in exon 62 of 71 total exons. The mutation also corresponds to residue 9710 in the NM_001252635 mRNA sequence in exon 61 of 70 total exons. The mutation results in a tyrosine (Y) to premature stop codon (*) substitution at amino acid residue 2923 in ENSMUSP00000101101 or residue 2921 in ENSMUSP00000101104. This model was generated at UT Southwestern Medical Center at Dallas.
Genotype Determination
Phenotype

Homozygous: The dee dee mutation was induced by N-ethyl-N-nitrosourea (ENU) mutagenesis and was discovered in G3 animals. Homozygous mutant mice exhibit excessive head bobbing. Additional Cdh23-associated phenotypes have not been examined.

Heterozygous: Not examined.

Mammalian Phenotype Terms
Allelic Composition: Cdh23m1Btlr/Cdh23m1Btlr (Genetic Background: C57BL/6J-Cdh23m1Btlr )

Strain Development
Original mutant was a C57BL/6J G3 ENU-induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
  • Cell Biology
  • Internal Organ
  • Models for Human Disease
    • Usher syndrome type 1D
  • Sensorineural
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference

Dismang A, Purrington T, Murray AR, and Beutler B. Record for dee dee. MUTAGENETIXTM, B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)

Wild-type female x Heterozygous male from the colony or reciprocal mating

Homozygous female x Homozygous male from the colony

Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
4-6
Recommended wean age
4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
036997-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.