Strain Detail Sheet

Strain Name    :

B6.129P2(Cg)-Izumo1rtm1.1Salb/Mmnc

Stock Number :

037093-UNC

Other Names   :

Folr4 KO

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Izumo1rtm1.1Salb
Name: targeted mutation 1.1, J Michael Salbaum
Chromosome: 9
Alteration at locus: Knockout
Gene Symbol: Izumo1r
Name: IZUMO1 receptor, JUNO
Chromosome: 9
Alteration at locus: Knockout

Genetic Alterations:
The first two coding exons were flanked by loxP sites and the second loxP site was followed by a neomycin resistance gene and a third loxP site. Properly targeted mice were crossed to mice expressing cre in oocytes to excise the selection cassette and the two floxed exons.

Genotype Determination:

    ES Cell Line: E14 derived from 129P2/OlaHsd

    Strain Description [Including phenotype, strain background, strain development and suggested control mice]

    Phenotype

    Homozygous: Female infertility due to deficiencies in the oocyte. Males not viable.

    Heterozygous: No observable phenotype

    Strain of Origin: 129P2/OlaHsd

    Strain genetic background: B6.129P2(Cg)

    Strain Development: Correctly targeted E14 ES cells derived from 129P2/OlaHsd were implanted into blastocysts. Chimeric males were mated to C57BL/6 mice. Generation of mice carrying the floxed allele were bred to homozygosity. Then a homozygous floxed male was bred with STOCK Tg(MMTV-cre)Mam/J female to generate offspring with full deletion of sequence flanked by loxP sites. Resulting offspring were backcrossed to C57BL/6 for 12 generations to make congenic. Strain then maintained by random intra-strain mating.

    Suggested Control Mice: Wild-type littermates or C57BL/6 mice

    Research Applications

    • Cell Biology
    • Developmental Biology
    • Immunology and Inflammation
    • Reproduction

    Strain Origin

    Donor: J. Michael Salbaum, Ph.D., Pennington Biomedical Research Center.

    Primary Reference:

    Salbaum JM, Kruger C, Kappen C. Mutation at the folate receptor 4 locus modulates gene expression profiles in the mouse uterus in response to periconceptional folate supplementation. Biochim Biophys Acta. 2013 Oct;1832(10):1653-61. (Medline PMID: 23651732)

    Colony and Husbandry Information

    Special Considerations

    None

    Health Status Report

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Appearance

    Coat color: Black

    Other:

    Breeding

    MMRRC Breeding System: Random intra-strain mating

    Breeding Scheme(s):

    • Wild-type female x Heterozygous male from the colony or reciprocal mating
    • Wild-type female x Hemizygous male from the colony or reciprocal mating

    Generation: N12+

    Overall Breeding Performance: Good

    Reproductive Statistics

    NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.

    Viability and Fertility:FemaleMale
    Homozygotes are viable: Yes No Gene is X-linked.
    Homozygotes are fertile: No No
    Hetero/Hemizygotes are fertile: Yes Yes

    Age Reproductive Decline: Undetermined Undetermined

    Average litter size: 4-6

    Recommended wean age: 4 weeks

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Conditions of Distribution [Including applicable technology transfer agreements]

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    - Products for this strain are Not Yet Available for Ordering -
    If you register interest in this strain, you will be notified when it becomes available for ordering.
    MMRRC Item # - Description Distribution
    Fee/unit (US $)
    Units Notes
    037093-UNC-RESUSLitter recovered from cryo-archive
    $2,022.00 / $4,109.00
    Non-Profit / For-Profit
    Litter Recovered litter1; additional fees for any special requests.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    This strain is not currently available. If you register interest in this strain, you will be notified when it becomes available for ordering (click Register Interest button above).



    The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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    Generated: 03/26/2015