Allele Symbol: Izumo1rtm1.1Salb
Name: targeted mutation 1.1, J Michael Salbaum
Alteration at locus: Knockout
Gene Symbol: Izumo1r
Name: IZUMO1 receptor, JUNO
Alteration at locus: Knockout
ES Cell Line: E14 derived from 129P2/OlaHsd
Homozygous: Female infertility due to deficiencies in the oocyte. Males not viable.
Heterozygous: No observable phenotype
Strain of Origin: 129P2/OlaHsd
Strain genetic background: B6.129P2(Cg)
Strain Development: Correctly targeted E14 ES cells derived from 129P2/OlaHsd were implanted into blastocysts. Chimeric males were mated to C57BL/6 mice. Generation of mice carrying the floxed allele were bred to homozygosity. Then a homozygous floxed male was bred with STOCK Tg(MMTV-cre)Mam/J female to generate offspring with full deletion of sequence flanked by loxP sites. Resulting offspring were backcrossed to C57BL/6 for 12 generations to make congenic. Strain then maintained by random intra-strain mating.
Suggested Control Mice: Wild-type littermates or C57BL/6 mice
Donor: J. Michael Salbaum, Ph.D., Pennington Biomedical Research Center.
Salbaum JM, Kruger C, Kappen C. Mutation at the folate receptor 4 locus modulates gene expression profiles in the mouse uterus in response to periconceptional folate supplementation. Biochim Biophys Acta. 2013 Oct;1832(10):1653-61. (Medline PMID: 23651732)
Coat color: Black
MMRRC Breeding System: Random intra-strain mating
Overall Breeding Performance: Good
|Viability and Fertility:||Female||Male||Comments|
|Homozygotes are viable:||Yes||No||Gene is X-linked.|
|Homozygotes are fertile:||No||No|
|Hetero/Hemizygotes are fertile:||Yes||Yes|
|Age Reproductive Decline:||Undetermined||Undetermined|
Average litter size: 4-6
Recommended wean age: 4 weeks
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
This strain is not currently available. If you register interest in this strain, you will be notified when it becomes available for ordering (click Register Interest button above).