Strain Name:
FVB.129S4-Hif1atm1Jhu/CkaMmjax
Stock Number:
037489-JAX
Citation ID:
RRID:MMRRC_037489-JAX
Other Names:
Hif1alpha on FVB

Gene Information

Hif1atm1Jhu
Name: hypoxia inducible factor 1, alpha subunit; targeted mutation 1, Johns Hopkins University
Synonyms: Hif1a-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Knockout
Hif1a
Name: hypoxia inducible factor 1, alpha subunit
Synonyms: MOP1, HIF-1alpha, HIF1alpha, bHLHe78
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Knockout
NCBI: 15251
HGNC: HGNC:4910
Homologene: 1171
Genetic Alterations

A targeting vector was designed to disrupt the mouse Hif1a gene by homologous recombination resulting in the replacement of exon 2 by a PGK promoter–neomycin resistance (neo) gene.

Genotype Determination
ES Cell Line
J1 derived from 129S4/SvJae
Phenotype

Homozygous: Embryonic lethal.

Hemizygous: FVB/N background allows for induction of diabetes.

Mammalian Phenotype Terms
Allelic Composition: Hif1atm1Jhu/Hif1atm1Jhu (Genetic Background: involves: 129S4/SvJae * C57BL/6 )

MeSH Terms
  • Animals
  • Blood Vessels/embryology
  • Cell Division/genetics
  • Cell Respiration/genetics
  • Cell Respiration/physiology
  • Cells, Cultured
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism
  • DNA-Binding Proteins/physiology
  • Embryo, Mammalian/metabolism
  • Embryo, Mammalian/pathology
  • Endothelial Growth Factors/genetics
  • Homeostasis/physiology
  • Hypoxia-Inducible Factor 1
  • Hypoxia-Inducible Factor 1, alpha Subunit
  • Immunoblotting
  • Immunohistochemistry
  • Lymphokines/genetics
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Nuclear Proteins/genetics
  • Nuclear Proteins/metabolism
  • Nuclear Proteins/physiology
  • Oxygen/metabolism
  • Platelet Endothelial Cell Adhesion Molecule-1/analysis
  • RNA, Messenger/analysis
  • Stem Cells/metabolism
  • Time Factors
  • Transcription Factors/physiology
  • Vascular Endothelial Growth Factor A
  • Vascular Endothelial Growth Factors
Strain Development

The Hif1a mutant allele was generated by injecting correctly targeted J1 ES cells derived from 129S4/SvJae into C57BL/6 blastocysts. Blastocysts were then surgically implanted into the uterus of pseudopregnant CD1 foster mothers to produce chimeras. Germ-line transmission was obtained when chimeric males were mated to C57BL/6 females. This allele was then backcrossed onto the FVB/N background for more than 10 generations.

Suggested Control Mice
Wild-type littermates or FVB/N mice
  • Cardiovascular
  • Developmental Biology
  • Diabetes
Donor
Claudia Kappen, Ph.D., Pennington Biomedical Research Center.
Primary Reference

Iyer NV, Kotch LE, Agani F, Leung SW, Laughner E, Wenger RH, Gassmann M, Gearhart JD, Lawler AM, Yu AY, Semenza GL. Cellular and developmental control of O2 homeostasis by hypoxia-inducible factor 1 alpha. Genes Dev. 1998 Jan 15;12(2):149-62. (Medline PMID: 9436976)

Colony and Husbandry Information

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Coat Color
White
MMRRC Breeding System
Backcross
Breeding Scheme(s)

Wild-type (FVB/N) female x Hemizygous male or reciprocal mating

Generation
N10+
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: Undetermined Undetermined
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
5-9
Recommended wean age
3-4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
037489-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
037489-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.