Strain Name:
NOD.Cg-Ptgs2tm1Hahe/PmfiMmjax
Stock Number:
037505-JAX
Citation ID:
RRID:MMRRC_037505-JAX
Other Names:
NOD.B10 COX-2flox/flox Line C

Gene Information

Ptgs2tm1Hahe
Name: prostaglandin-endoperoxide synthase 2; targeted mutation 1, Harvey R Herschman
Synonyms: Ptgs2tm1Hrh, Cox-2flox
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Conditional
Ptgs2
Name: prostaglandin-endoperoxide synthase 2
Synonyms: Cox-2, cyclooxygenase 2, Pghs2, COX2, prostaglandin G/H synthase, PHS-2, cyclooxygenase-2, PGHS-2, Tis10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Conditional
NCBI: 19225
HGNC: HGNC:9605
Homologene: 31000
Genetic Alterations
A targeting vector was designed to flank exons 4 and 5 with loxP sites. Deletion of these exons would cause a frame shift, resulting in a stop codon after eight missense amino acids in exon 6.
Genotype Determination
ES Cell Line
LW1 derived from 129S4/SvJae
Phenotype

Homozygous: No observable phenotype

Cre-excised Phenotype: Exposure of NOD.B10 COX-2flox splenocytes to recombinant HTN-Cre results in dose-dependent deletion of the floxed Ptgs2 gene within one hour of exposure. Earlier work showed that targeted in vivo expression of Cre in myeloid cells of C57BL/6 mice can block Ptgs2 expression in macrophages and attenuate acute inflammation. One objective of this laboratory is to discern whether B cell-targeted Cre excision of COX-2flox affects B cell function in vitro and B cell-driven autoimmune disease in vivo. Unfortunately, recent efforts to backcross a CD19cre gene onto the NOD.B10 background resulted congenic mice which failed to manifest Sjogren's syndrome hyposalivation (note: this occurred in the absence of COX-2flox); additionally, the latter strain failed to breed well and was lost. Further efforts in this direction would require backcrossing another gene for B cell-targeted Cre expression.

Mammalian Phenotype Terms
Allelic Composition: Ptgs2tm1Hahe/Ptgs2tm1Hahe (Genetic Background: involves: 129S4/SvJae )

MeSH Terms
  • Animals
  • Chromosome Mapping
  • Chromosomes, Mammalian
  • Cyclooxygenase 2/genetics
  • Disease Models, Animal
  • Female
  • Gene Expression
  • Genetic Predisposition to Disease
  • Humans
  • Male
  • Mice
  • Mice, Congenic
  • Mice, Inbred NOD
  • Open Reading Frames
  • Recombination, Genetic
  • Salivary Glands/metabolism
  • Salivary Glands/pathology
  • Sialadenitis/genetics
  • Sialadenitis/immunology
  • Sialadenitis/pathology
  • Sjogren's Syndrome/complications
  • Sjogren's Syndrome/genetics
  • Sjogren's Syndrome/immunology
  • Xerostomia/etiology
Strain Development
A floxed Ptgs2 gene LW1 derived from 129S4/SvJae was developed by and bred onto a 129xB6 background. The germline floxed Ptgs2 allele was maintained on a mixed 129xB6 background prior to being backcrossed onto the Sjogren's syndrome-susceptible NOD.B10Sn-H2b/J background through speed-congenic breeding. Because the Ptgs2 gene was localed in the center of an earlier mapped Aec2 disease susceptiblity region for Sjogren's syndrome in NOD.B10Sn-H2b/J mice, additional backcross generations were needed to recover the SS xerostomia phenotype.
Suggested Control Mice
Wild-type littermates
  • Diabetes
  • Immunology and Inflammation
  • Models for Human Disease
    • Sjorgen's Syndrome
  • Neurobiology
Donor
Patricia Mongini, Ph.D., The Feinstein Institute for Medical Research.
Primary Reference

Ishikawa TO, Herschman HR. Conditional knockout mouse for tissue-specific disruption of the cyclooxygenase-2 (Cox-2) gene. Genesis. 2006 Mar;44(3):143-9. (Medline PMID:16496341)

Mongini PK, Kramer JM, Ishikawa TO, Herschman H, Esposito D. Candidate chromosome 1 disease susceptiblity genes for Sjogren's syndrome xerostomia are narrowed by novel NOD.B10 congenic mice. Clin Immunol. 2014 Jul;153(1):79-90. (Medline PMID: 24685748)

Colony and Husbandry Information

This mouse strain appears to have a nervous disposition. This might explain why breeding has not been as routinely successful at the Feinstein Institute as at Charles River breeding facility (categorized as good). It might also explain why we have seen evidence of cannibalism of the litters here at the Feinstein Institute. The individual in charge of their breeding at Charles River GEMS (Karen.Dorman@crl.com) has indicated that some (but not all) of the breeders declined in productivity after 1-2 litters. Because of this, she breeders were typically replaced after 3-4 months of breeding.
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Coat Color
White
MMRRC Breeding System
Backcross
Generation
N7
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 5 months Undetermined
Average litter size
Less than 4
Recommended wean age
3-4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
037505-JAX-EMBRYO Cryo-preserved embryos $1,038.00 / $1,038.00
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
037505-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / $2,022.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.