Strain Name:
C57BL/6J-Pik3cdm1Btlr/Mmmh
Stock Number:
037532-MU
Citation ID:
RRID:MMRRC_037532-MU
Other Names:
stinger
Major Collection:

Gene Information

Allele Symbol: Pik3cdm1Btlr (Mus musculus (mouse))
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta; mutation 1, Bruce Beutler
Synonyms: stinger
Chromosome: 4
Alteration at locus: Chemically Induced
Monarch Initiative (Disease and Phenotype Associations): MGI:5582742
Gene Symbol: Pik3cd (Mus musculus (mouse))
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta
Synonyms: 2410099E07Rik, p110delta, 2610208K16Rik
Chromosome: 4
Alteration at locus: Chemically Induced
Monarch Initiative (Disease and Phenotype Associations): MGI:1098211
Genetic Alterations
The stinger mutation is a T to G transversion at base pair 149,657,319 (v38) on chromosome 4, or base pair 68,648 in the GenBank genomic region NC_000070 encoding Pik3cd. The mutation corresponds to residue 1447 in the mRNA sequence NM_001029837 within exon 8 of 23 total exons. The mutation results in a leucine (L) to arginine (R) substitution at position 390 (L390R) in all of the Pik3d isoforms.
Genotype Determination
ES Cell Line
N/A
Phenotype

Homozygous: The stinger phenotype was identified among G3 mice of the pedigree F5770, some of which exhibited a null T-independent IgM response to 4-hydroxy-3-nitrophenylacetyl-Ficoll (NP-Ficoll) and a null T-dependent IgG response to recombinant Semliki Forest virus (rSFV)-encoded β-galactosidase (rSFV-β-gal).

Heterozygous: Not tested

Mammalian Phenotype Terms
Allelic Composition: Pik3cdm1Btlr/Pik3cdm1Btlr (Genetic Background: C57BL/6J-Pik3cdm1Btlr )

  • immune system
MeSH Terms
    Strain Development
    Original mutant was a C57BL/6J G3 ENU-induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice
    • Cancer
    • Cell Biology
    • Developmental Biology
    • Hematology
    • Immunology and Inflammation
    • Models for Human Disease
      • Cancer
      • Rheumatoid arthritis
      • Asthma
    • Neurobiology
    • Reproduction
    • Research Tools
    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference

    Arnold C, Choi JH, Pirie E, Wang K-H, Zeng M, Murray AR, and Beutler B. Record for stinger. MUTAGENETIXtm, B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    • Wild-type female x Heterozygous male from the colony or reciprocal mating
    • Homozygous female x Homozygous male from the colony
    Overall Breeding Performance
    Good
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Undetermined Undetermined
    Average litter size
    4-6
    Recommended wean age
    3-4 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    037532-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.