Strain Detail Sheet
Zp2tm1Dean: Part of exon 1 and exon 2 were replaced by a neomycin cassette. Absence of the protein was confirmed by immunohistochemistry on ovarian sections of mutant female mice
Tg(Zp2*)2Dean: The full-length sequence of the mouse Zp2 gene in BAC RP23-6513 was modified, putting in place the human exons encoding amino acids 41-68 (bp 26 in exon 2 to bp 21 in exon 6) for the corresponding exons encoding the amino acids 37-165 (bp 59 in exon 2 to bp 6 in exon 5).
Homozygous: Ovulated eggs are surrounded by a zona pellucida composed by mouse Zp1, mouse Zp3 and chimeric human/mouse Zp2 (replacing normal mouse Zp2) in which the first 150 amino acids of mouse Zp2 have been replaced by recombineering with the first 150 amino acids from human ZP2. As a result, both human and mouse sperm can bind to these eggs.
Hetero/Hemizygous: No phenotype
Allelic Composition: Zp2tm1Dean/Zp2tm1Dean (Genetic Background: involves: 129S1/Sv * 129X1/SvJ * CF-1 )- endocrine/exocrine glands
- abnormal cumulus oophorus [MP:0009372] [PMID:11245577]
- abnormal secondary ovarian follicle morphology [MP:0009363] [PMID:11245577]
- reproductive system
- abnormal cumulus oophorus [MP:0009372] [PMID:11245577]
- abnormal secondary ovarian follicle morphology [MP:0009363] [PMID:11245577]
- thin zona pellucida [MP:0009375] [PMID:11245577]
- abnormal ovulation [MP:0001928] [PMID:11245577]
- female infertility [MP:0001926] [PMID:11245577]
- cellular
- abnormal cumulus oophorus [MP:0009372] [PMID:11245577]
- Animals
- Egg Proteins/chemistry
- Egg Proteins/genetics
- Egg Proteins/metabolism
- Female
- Humans
- Cell Biology
- Developmental Biology
- Reproduction
Avella MA, Baibakov, B and Dean J. A single domain of the ZP2 zona pellucida protein mediates gamete recognition in mice and humans. J Cell Biol. 2014 Jun 23;205(6):801-9. Epub 2014 Jun 16 (Medline PMID: 24934154)
Varied
- Wild-type female x Hemizygous male littermate or reciprocal mating
- Hemizygous female x Hemizygous male littermate
| Viability and Fertility: | Female | Male | Comments |
|---|---|---|---|
| Homozygotes are viable: | Yes | Yes | |
| Homozygotes are fertile: | Reduced | Yes | |
| Hetero/Hemizygotes are fertile: | Yes | Yes | |
| Age Reproductive Decline: | 5 months | 5 months |
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
| MMRRC Item # | Description | Distribution Fee / Unit (US $) *Shipping & Handling not included* |
Units | Notes |
|---|---|---|---|---|
| 037594-JAX-SPERM | Cryo-preserved spermatozoa | $437.00 / Non-Profit | Aliquot | Approximate quantity3 |
| 037594-JAX-RESUS | Litter recovered from cryo-archive | $2,022.00 / Non-Profit | Litter | Recovered litter4; additional fees for any special requests. |
| Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information. | ||||
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.