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Strain Name:
C57BL/6J-MtgxR0047Btlr/Mmmh
Stock Number:
038341-MU
Citation ID:
RRID:MMRRC_038341-MU
Other Names:
R0047 (G1), C57BL/6J-MtgxR0047Btlr
Major Collection:

Strain Information

Myo5a
Name: myosin VA
Synonyms: MVa, MyoVA, Myo5, flail, 9630007J19Rik, Dbv
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17918
HGNC: HGNC:7602
Homologene: 20100
Tln2
Name: talin 2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 70549
VEGA: 9
Homologene: 56692
Ext1
Name: exostosin glycosyltransferase 1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 14042
HGNC: HGNC:3512
Homologene: 30957
Lamb1
Name: laminin B1
Synonyms: Lamb-1, C81607, C80098, D130003D08Rik, Lamb1-1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 16777
HGNC: HGNC:6486
Homologene: 1722
Lpp
Name: LIM domain containing preferred translocation partner in lipoma
Synonyms: B130055L10Rik, 9430020K16Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 210126
VEGA: 16
HGNC: HGNC:6679
Homologene: 4075
Cnot7
Name: CCR4-NOT transcription complex, subunit 7
Synonyms: Caf1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 18983
Homologene: 49011
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 74,946,591 bp
  • G to A, chromosome 1 at 160,231,789 bp
  • A to T, chromosome 2 at 25,011,606 bp
  • A to G, chromosome 2 at 29,084,655 bp
  • A to G, chromosome 2 at 110,268,302 bp
  • T to A, chromosome 2 at 122,346,641 bp
  • C to T, chromosome 2 at 127,234,954 bp
  • T to G, chromosome 2 at 181,323,405 bp
  • T to C, chromosome 3 at 18,055,569 bp
  • T to C, chromosome 3 at 19,694,037 bp
  • T to A, chromosome 3 at 36,908,192 bp
  • A to G, chromosome 3 at 96,684,063 bp
  • G to T, chromosome 3 at 106,115,257 bp
  • A to T, chromosome 3 at 135,595,053 bp
  • A to G, chromosome 3 at 138,066,264 bp
  • A to G, chromosome 4 at 59,914,392 bp
  • A to G, chromosome 4 at 117,761,032 bp
  • T to C, chromosome 4 at 138,743,590 bp
  • A to G, chromosome 5 at 101,944,033 bp
  • A to T, chromosome 5 at 119,680,446 bp
  • A to T, chromosome 5 at 135,911,299 bp
  • T to C, chromosome 5 at 136,363,253 bp
  • A to G, chromosome 5 at 137,403,656 bp
  • A to G, chromosome 6 at 35,287,362 bp
  • A to G, chromosome 6 at 42,629,613 bp
  • G to A, chromosome 6 at 43,265,621 bp
  • G to T, chromosome 6 at 83,182,632 bp
  • G to A, chromosome 6 at 92,905,306 bp
  • A to T, chromosome 6 at 100,733,378 bp
  • G to A, chromosome 6 at 112,374,820 bp
  • G to T, chromosome 6 at 113,672,953 bp
  • G to A, chromosome 6 at 113,678,738 bp
  • A to T, chromosome 7 at 16,561,992 bp
  • A to G, chromosome 7 at 17,677,881 bp
  • T to A, chromosome 7 at 26,766,826 bp
  • G to A, chromosome 7 at 28,890,387 bp
  • T to A, chromosome 7 at 45,336,689 bp
  • T to C, chromosome 7 at 86,811,650 bp
  • A to G, chromosome 7 at 102,009,453 bp
  • A to T, chromosome 7 at 103,552,322 bp
  • A to G, chromosome 7 at 108,630,552 bp
  • A to G, chromosome 8 at 3,202,947 bp
  • A to G, chromosome 8 at 40,495,921 bp
  • C to T, chromosome 8 at 46,041,365 bp
  • C to T, chromosome 8 at 84,176,134 bp
  • T to C, chromosome 8 at 107,036,701 bp
  • A to T, chromosome 8 at 111,165,582 bp
  • T to C, chromosome 9 at 7,451,910 bp
  • T to A, chromosome 9 at 21,388,714 bp
  • T to A, chromosome 9 at 23,406,686 bp
  • C to T, chromosome 9 at 50,861,573 bp
  • A to G, chromosome 9 at 67,240,672 bp
  • T to A, chromosome 9 at 75,156,207 bp
  • C to A, chromosome 9 at 106,196,696 bp
  • T to C, chromosome 9 at 108,054,658 bp
  • A to G, chromosome 9 at 110,485,038 bp
  • T to A, chromosome 10 at 3,978,727 bp
  • T to C, chromosome 10 at 30,535,243 bp
  • A to G, chromosome 10 at 88,675,941 bp
  • A to T, chromosome 10 at 127,903,672 bp
  • C to T, chromosome 11 at 23,464,403 bp
  • T to A, chromosome 11 at 54,546,378 bp
  • C to T, chromosome 11 at 61,336,242 bp
  • T to A, chromosome 11 at 67,367,237 bp
  • T to C, chromosome 11 at 94,569,342 bp
  • A to C, chromosome 11 at 95,300,208 bp
  • T to A, chromosome 11 at 98,997,856 bp
  • T to C, chromosome 12 at 31,278,601 bp
  • C to A, chromosome 12 at 35,101,124 bp
  • T to G, chromosome 12 at 71,075,419 bp
  • G to T, chromosome 12 at 76,622,950 bp
  • T to A, chromosome 12 at 104,240,284 bp
  • T to A, chromosome 12 at 113,288,168 bp
  • A to T, chromosome 13 at 12,222,226 bp
  • A to T, chromosome 13 at 32,850,276 bp
  • T to A, chromosome 13 at 59,645,100 bp
  • A to G, chromosome 13 at 62,274,471 bp
  • A to G, chromosome 13 at 95,010,287 bp
  • T to C, chromosome 14 at 20,704,163 bp
  • G to A, chromosome 14 at 20,771,068 bp
  • T to G, chromosome 14 at 30,346,790 bp
  • C to T, chromosome 14 at 31,077,045 bp
  • T to A, chromosome 14 at 104,503,344 bp
  • T to G, chromosome 15 at 7,253,430 bp
  • T to C, chromosome 15 at 39,878,239 bp
  • T to C, chromosome 15 at 53,345,146 bp
  • A to G, chromosome 15 at 85,862,378 bp
  • T to A, chromosome 16 at 22,987,563 bp
  • T to A, chromosome 16 at 24,661,800 bp
  • T to A, chromosome 16 at 59,027,224 bp
  • C to T, chromosome 16 at 59,269,211 bp
  • T to C, chromosome 17 at 36,857,864 bp
  • C to A, chromosome 17 at 48,364,980 bp
  • A to T, chromosome 17 at 56,955,624 bp
  • A to T, chromosome 17 at 67,795,186 bp
  • T to G, chromosome 17 at 80,550,845 bp
  • A to T, chromosome 18 at 37,321,268 bp
  • T to C, chromosome 18 at 43,313,378 bp
  • C to T, chromosome 18 at 82,680,692 bp
  • A to C, chromosome 19 at 7,283,577 bp
  • A to G, chromosome 19 at 13,502,589 bp
  • T to A, chromosome 19 at 24,684,556 bp
  • A to C, chromosome X at 37,607,469 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0047 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038341-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.