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Strain Name:
C57BL/6J-MtgxR0211Btlr/Mmmh
Stock Number:
038462-MU
Citation ID:
RRID:MMRRC_038462-MU
Other Names:
R0211 (G1), C57BL/6J-MtgxR0211Btlr
Major Collection:

Strain Information

Prkar2b
Name: protein kinase, cAMP dependent regulatory, type II beta
Synonyms: Pkarb2, RII(beta), PKARIIbeta
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 19088
HGNC: HGNC:9392
Homologene: 37666
Ltbp3
Name: latent transforming growth factor beta binding protein 3
Synonyms: Ltbp2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 16998
VEGA: 19
HGNC: HGNC:6716
Homologene: 7405
Arhgef12
Name: Rho guanine nucleotide exchange factor 12
Synonyms: LARG, 2310014B11Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 69632
VEGA: 9
Homologene: 9088
Tmem63b
Name: transmembrane protein 63b
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224807
Homologene: 101682
Ggnbp2
Name: gametogenetin binding protein 2
Synonyms: DIF-3, Zfp403, D330017P12Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217039
Homologene: 11750
Fuz
Name: fuzzy planar cell polarity protein
Synonyms: 2600013E07Rik, b2b1273Clo
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 70300
Homologene: 11840
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 36,346,037 bp
  • T to A, chromosome 1 at 100,478,374 bp
  • T to C, chromosome 1 at 174,028,428 bp
  • T to C, chromosome 2 at 32,919,604 bp
  • A to C, chromosome 2 at 86,423,107 bp
  • T to C, chromosome 2 at 91,630,158 bp
  • A to G, chromosome 2 at 119,038,412 bp
  • A to T, chromosome 2 at 160,898,681 bp
  • T to A, chromosome 2 at 164,777,484 bp
  • T to C, chromosome 2 at 164,907,692 bp
  • T to A, chromosome 3 at 95,476,149 bp
  • C to T, chromosome 3 at 146,650,496 bp
  • T to C, chromosome 3 at 154,587,495 bp
  • C to T, chromosome 3 at 155,055,344 bp
  • T to A, chromosome 4 at 56,795,545 bp
  • C to T, chromosome 4 at 94,679,255 bp
  • T to G, chromosome 4 at 109,073,124 bp
  • T to A, chromosome 4 at 118,934,270 bp
  • C to G, chromosome 4 at 130,378,043 bp
  • G to A, chromosome 4 at 138,309,602 bp
  • A to G, chromosome 4 at 145,114,778 bp
  • T to C, chromosome 5 at 29,785,079 bp
  • T to A, chromosome 5 at 43,688,266 bp
  • T to G, chromosome 5 at 92,057,964 bp
  • T to A, chromosome 5 at 146,295,239 bp
  • T to A, chromosome 5 at 146,483,060 bp
  • G to T, chromosome 6 at 24,633,893 bp
  • A to T, chromosome 6 at 47,541,835 bp
  • A to T, chromosome 6 at 87,749,154 bp
  • T to G, chromosome 6 at 125,141,344 bp
  • T to A, chromosome 6 at 142,688,984 bp
  • C to A, chromosome 6 at 146,194,613 bp
  • C to T, chromosome 7 at 4,373,209 bp
  • T to A, chromosome 7 at 37,568,449 bp
  • T to A, chromosome 7 at 44,899,022 bp
  • A to C, chromosome 7 at 45,288,243 bp
  • A to G, chromosome 7 at 128,204,641 bp
  • A to G, chromosome 8 at 84,908,788 bp
  • T to A, chromosome 8 at 109,541,740 bp
  • A to T, chromosome 8 at 120,739,975 bp
  • T to A, chromosome 8 at 121,116,616 bp
  • T to A, chromosome 9 at 22,200,173 bp
  • T to C, chromosome 9 at 31,157,790 bp
  • G to A, chromosome 9 at 42,972,004 bp
  • T to C, chromosome 9 at 50,861,625 bp
  • A to T, chromosome 9 at 72,961,367 bp
  • A to T, chromosome 9 at 100,497,767 bp
  • T to C, chromosome 9 at 109,089,373 bp
  • T to A, chromosome 9 at 109,103,663 bp
  • T to A, chromosome 10 at 77,038,466 bp
  • T to A, chromosome 10 at 122,449,469 bp
  • A to G, chromosome 10 at 127,121,036 bp
  • A to T, chromosome 11 at 40,714,808 bp
  • T to C, chromosome 11 at 62,714,917 bp
  • A to G, chromosome 11 at 69,855,181 bp
  • C to T, chromosome 11 at 70,974,059 bp
  • T to A, chromosome 11 at 77,718,867 bp
  • T to G, chromosome 11 at 80,095,647 bp
  • G to A, chromosome 11 at 84,840,313 bp
  • T to A, chromosome 11 at 88,982,153 bp
  • T to G, chromosome 11 at 95,571,912 bp
  • A to T, chromosome 11 at 116,265,551 bp
  • T to A, chromosome 11 at 120,730,788 bp
  • C to A, chromosome 12 at 31,972,184 bp
  • T to G, chromosome 12 at 70,014,875 bp
  • T to C, chromosome 12 at 71,216,096 bp
  • A to T, chromosome 12 at 76,097,957 bp
  • T to A, chromosome 12 at 83,535,961 bp
  • T to A, chromosome 12 at 103,072,792 bp
  • T to C, chromosome 12 at 112,655,142 bp
  • C to G, chromosome 13 at 67,293,147 bp
  • T to G, chromosome 14 at 37,046,968 bp
  • A to T, chromosome 14 at 55,787,918 bp
  • T to C, chromosome 14 at 55,871,798 bp
  • A to G, chromosome 14 at 65,032,208 bp
  • A to T, chromosome 15 at 58,939,282 bp
  • C to A, chromosome 15 at 101,922,782 bp
  • A to G, chromosome 16 at 9,579,173 bp
  • T to C, chromosome 16 at 96,716,079 bp
  • T to A, chromosome 17 at 6,050,060 bp
  • A to T, chromosome 17 at 15,021,943 bp
  • C to T, chromosome 17 at 27,714,920 bp
  • C to T, chromosome 17 at 28,318,160 bp
  • T to A, chromosome 17 at 35,864,611 bp
  • T to C, chromosome 17 at 36,168,007 bp
  • C to T, chromosome 17 at 43,038,213 bp
  • T to C, chromosome 17 at 43,296,690 bp
  • T to A, chromosome 17 at 45,661,913 bp
  • C to T, chromosome 17 at 56,102,242 bp
  • T to A, chromosome 17 at 71,729,248 bp
  • C to T, chromosome 17 at 72,603,516 bp
  • C to T, chromosome 17 at 80,644,841 bp
  • A to G, chromosome 18 at 11,720,535 bp
  • A to T, chromosome 18 at 37,414,006 bp
  • C to T, chromosome 19 at 4,620,199 bp
  • T to G, chromosome 19 at 4,856,164 bp
  • T to C, chromosome 19 at 5,752,143 bp
  • T to C, chromosome 19 at 13,028,282 bp
  • A to G, chromosome 19 at 45,033,667 bp
  • A to G, chromosome 19 at 46,670,776 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0211 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038462-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.