Strain Name:
C57BL/6J-MtgxR0269Btlr/Mmmh
Stock Number:
038495-MU
Citation ID:
RRID:MMRRC_038495-MU
Other Names:
R0269 (G1), C57BL/6J-MtgxR0269Btlr
Major Collection:

Gene Information

Smarca4
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Synonyms: b2b692Clo, SW1/SNF, b2b508.1Clo, Brg1, SNF2beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 20586
Homologene: 135927
Sema6d
Name: sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D
Synonyms: Sema6D-2, Sema6D-4, Sema6D-1, Sema6D-6, 1110067B02Rik, Sema6D-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 214968
Homologene: 16195
Gldc
Name: glycine decarboxylase
Synonyms: D030049L12Rik, b2b2679Clo, D19Wsu57e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 104174
VEGA: 19
HGNC: HGNC:4313
Homologene: 141
Rgs7
Name: regulator of G protein signaling 7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 24012
Homologene: 2193
Ntrk1
Name: neurotrophic tyrosine kinase, receptor, type 1
Synonyms: Tkr, TrkA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 18211
HGNC: HGNC:8031
Homologene: 1898
Hcn2
Name: hyperpolarization-activated, cyclic nucleotide-gated K+ 2
Synonyms: HAC1, trls
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 15166
HGNC: HGNC:4846
Homologene: 31022
Sgsm1
Name: small G protein signaling modulator 1
Synonyms: D5Bwg1524e, 2410098H20Rik, Rutbc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 52850
Homologene: 64485
Kcnq2
Name: potassium voltage-gated channel, subfamily Q, member 2
Synonyms: KQT2, Nmf134
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 16536
HGNC: HGNC:6296
Homologene: 26174
Tut7
Name: terminal uridylyl transferase 7
Synonyms: Zcchc6, Tent3b, 6030448M23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 214290
VEGA: 13
Homologene: 51941
Fbxl17
Name: F-box and leucine-rich repeat protein 17
Synonyms: C130023C01Rik, Fbx13, 6330576B01Rik, Fbxo13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 50758
Homologene: 79859
Smg6
Name: Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 103677
Homologene: 23024
Abca1
Name: ATP-binding cassette, sub-family A (ABC1), member 1
Synonyms: ABC1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 11303
HGNC: HGNC:29
Homologene: 21130
Amhr2
Name: anti-Mullerian hormone type 2 receptor
Synonyms: MIS TypeII receptor, MISIIR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 110542
HGNC: HGNC:465
Homologene: 10746
Mlh3
Name: mutL homolog 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 217716
VEGA: 12
HGNC: HGNC:7128
Homologene: 91153
Mgat4a
Name: mannoside acetylglucosaminyltransferase 4, isoenzyme A
Synonyms: 9530018I07Rik, GnT-IVa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 269181
HGNC: HGNC:7047
Homologene: 8153
Csf2rb2
Name: colony stimulating factor 2 receptor, beta 2, low-affinity (granulocyte-macrophage)
Synonyms: AIC2A, Il3r, BetaIl3, Bil3, Il3rb2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 12984
VEGA: 15
HGNC: HGNC:2436
Homologene: 339
Recql5
Name: RecQ protein-like 5
Synonyms: Recql5b, Recq5b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 170472
HGNC: HGNC:9950
Homologene: 31232
Myadm
Name: myeloid-associated differentiation marker
Synonyms: D7Wsu62e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 50918
HGNC: HGNC:7544
Homologene: 9668
Nol8
Name: nucleolar protein 8
Synonyms: 4921532D18Rik, 5730412B09Rik, D13Ertd548e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 70930
VEGA: 13
Homologene: 41210
Bpifb1
Name: BPI fold containing family B, member 1
Synonyms: von Ebner minor salivary protein, U46068, LPlunc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 228801
Homologene: 50047
Kdelr1
Name: KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1
Synonyms: 8030486F04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 68137
HGNC: HGNC:6304
Homologene: 38236
Stxbp1
Name: syntaxin binding protein 1
Synonyms: Munc-18a, nsec1, Sxtbp1, Munc18-1, Unc18h, N-sec1, Rb-sec1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 20910
Homologene: 2382
Reln
Name: reelin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 19699
HGNC: HGNC:9957
Homologene: 3699
Kidins220
Name: kinase D-interacting substrate 220
Synonyms: 3110039L19Rik, C330002I19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 77480
VEGA: 12
Homologene: 14254
Ush2a
Name: usherin
Synonyms: Ush2a, Ushrn, MUSH2A, A930037M10Rik, A930011D15Rik, LOC269160, LOC381317
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 22283
Homologene: 66151
Tm4sf5
Name: transmembrane 4 superfamily member 5
Synonyms: 2010003F10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 75604
Homologene: 2947
Oog3
Name: oogenesin 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 100012
Homologene: 129883
Rabl6
Name: RAB, member RAS oncogene family-like 6
Synonyms: Rbel1a, Rbel1b, Rbel1, B230208H17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 227624
Homologene: 11674
Arrb1
Name: arrestin, beta 1
Synonyms: beta-arrestin1, 1200006I17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 109689
HGNC: HGNC:711
Homologene: 2981
Olfr954
Name: olfactory receptor 954
Synonyms: MOR171-53, GA_x6K02T2PVTD-33158015-33158950, MOR171-42
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 258328
VEGA: 9
Homologene: 71961
Adcy2
Name: adenylate cyclase 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 210044
VEGA: 13
HGNC: HGNC:233
Homologene: 75133
Trmt11
Name: tRNA methyltransferase 11
Synonyms: 3110045I18Rik, 2410075D05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 73681
VEGA: 10
Homologene: 6876
Guf1
Name: GUF1 homolog, GTPase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 231279
Homologene: 6505
Sytl2
Name: synaptotagmin-like 2
Synonyms: Slp2-a, Slp2-c, Slp2, Slp2-d, Slp2-b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 83671
Homologene: 131343
Prss39
Name: protease, serine 39
Synonyms: Tesp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 21755
Homologene: 84781
AW551984
Name: expressed sequence AW551984
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 244810
Chil3
Name: chitinase-like 3
Synonyms: Chi3l3, Ym1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 12655
Homologene: 74931
Spata17
Name: spermatogenesis associated 17
Synonyms: 4930504I07Rik, 1700065F16Rik, 4930513F16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 74717
Homologene: 12551
Alk
Name: anaplastic lymphoma kinase
Synonyms: CD246, Tcrz
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 11682
VEGA: 17
HGNC: HGNC:427
Homologene: 68387
D130040H23Rik
Name: RIKEN cDNA D130040H23 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 211135
Homologene: 138403
Tmx2
Name: thioredoxin-related transmembrane protein 2
Synonyms: Txndc14, 2310042M24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 66958
Homologene: 9317
Slc6a21
Name: solute carrier family 6 member 21
Synonyms: 1700039E15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 76713
Homologene: 66347
Bpifb9b
Name: BPI fold containing family B, member 9B
Synonyms: OTTMUSG00000015915, 5430413K10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 433492
Homologene: 128535
Cd46
Name: CD46 antigen, complement regulatory protein
Synonyms: CD46, Mcp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 17221
HGNC: HGNC:6953
Homologene: 7832
Olfr1036
Name: olfactory receptor 1036
Synonyms: GA_x6K02T2Q125-47549689-47550621, MOR245-25, MOR262-13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258245
Olfr1196
Name: olfactory receptor 1196
Synonyms: MOR225-5, GA_x6K02T2Q125-50181139-50180195
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258456
Homologene: 123770
Laptm5
Name: lysosomal-associated protein transmembrane 5
Synonyms: E3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 16792
Homologene: 4922
Pramef17
Name: PRAME family member 17
Synonyms: Gm13107
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 626943
Homologene: 129642
Sult1d1
Name: sulfotransferase family 1D, member 1
Synonyms: tyrosine-ester sulfotransferase, 5033411P13Rik, Sultn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 53315
Homologene: 124464
Defb12
Name: defensin beta 12
Synonyms: 9230103N16Rik, mBD-12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 77674
Homologene: 17531
Cdkn2aip
Name: CDKN2A interacting protein
Synonyms: 4921511I16Rik, CARF
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 70925
Homologene: 9752
Hddc2
Name: HD domain containing 2
Synonyms: 2310057G13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 69692
Homologene: 5431
Olfr313
Name: olfactory receptor 313
Synonyms: GA_x6K02T2NKPP-590035-589109, MOR222-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 258529
Homologene: 77375
Olfr466
Name: olfactory receptor 466
Synonyms: GA_x6K02T2PB7A-3051266-3052192, MOR209-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 258816
Homologene: 72032
5031439G07Rik
Name: RIKEN cDNA 5031439G07 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 223739
HGNC: HGNC:1314
Homologene: 15140
Fam234a
Name: family with sequence similarity 234, member A
Synonyms: Itfg3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 106581
Homologene: 12932
Zfp422-ps
Name: zinc finger protein 422, pseudogene
Synonyms: Zfp422-rs1, Krox-25-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 100416808
Slc22a19
Name: solute carrier family 22 (organic anion transporter), member 19
Synonyms: D630043A20Rik, Slc22a9, Oat5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 207151
Homologene: 133125
Cyp2c40
Name: cytochrome P450, family 2, subfamily c, polypeptide 40
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 13099
Homologene: 74936
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 34,500,198 bp
  • A to G, chromosome 1 at 37,490,307 bp
  • A to G, chromosome 1 at 175,270,820 bp
  • T to C, chromosome 1 at 187,097,872 bp
  • T to A, chromosome 1 at 188,810,176 bp
  • T to G, chromosome 1 at 195,064,688 bp
  • A to G, chromosome 2 at 25,586,866 bp
  • A to C, chromosome 2 at 32,802,783 bp
  • T to C, chromosome 2 at 84,672,396 bp
  • A to G, chromosome 2 at 86,075,141 bp
  • A to G, chromosome 2 at 88,700,696 bp
  • T to A, chromosome 2 at 124,660,745 bp
  • A to G, chromosome 2 at 154,212,947 bp
  • C to T, chromosome 2 at 154,319,625 bp
  • T to C, chromosome 2 at 181,096,974 bp
  • T to C, chromosome 3 at 87,783,933 bp
  • T to C, chromosome 3 at 106,155,756 bp
  • T to C, chromosome 4 at 53,044,228 bp
  • A to T, chromosome 4 at 130,930,816 bp
  • A to G, chromosome 4 at 143,993,518 bp
  • A to T, chromosome 4 at 144,160,214 bp
  • T to C, chromosome 5 at 21,920,537 bp
  • A to C, chromosome 5 at 69,559,599 bp
  • A to T, chromosome 5 at 87,564,802 bp
  • T to C, chromosome 5 at 113,286,929 bp
  • A to G, chromosome 7 at 3,296,757 bp
  • T to A, chromosome 7 at 45,286,908 bp
  • T to A, chromosome 7 at 45,874,039 bp
  • T to C, chromosome 7 at 90,403,020 bp
  • T to C, chromosome 7 at 99,594,677 bp
  • G to T, chromosome 8 at 19,114,359 bp
  • A to G, chromosome 8 at 47,711,977 bp
  • T to C, chromosome 8 at 69,300,794 bp
  • T to G, chromosome 9 at 21,636,201 bp
  • T to C, chromosome 9 at 39,461,794 bp
  • T to C, chromosome 9 at 39,599,950 bp
  • T to A, chromosome 10 at 30,587,489 bp
  • A to G, chromosome 10 at 31,327,946 bp
  • A to G, chromosome 10 at 79,734,241 bp
  • T to C, chromosome 11 at 58,817,149 bp
  • T to A, chromosome 11 at 70,510,669 bp
  • C to A, chromosome 11 at 75,162,931 bp
  • T to C, chromosome 11 at 115,928,224 bp
  • T to A, chromosome 12 at 25,040,512 bp
  • C to A, chromosome 12 at 85,268,405 bp
  • T to C, chromosome 13 at 49,654,445 bp
  • A to T, chromosome 13 at 59,816,855 bp
  • A to T, chromosome 13 at 65,152,878 bp
  • T to A, chromosome 13 at 68,678,606 bp
  • G to T, chromosome 15 at 78,288,865 bp
  • C to T, chromosome 15 at 84,954,000 bp
  • T to C, chromosome 15 at 102,447,068 bp
  • A to T, chromosome 17 at 26,216,617 bp
  • A to T, chromosome 17 at 33,305,463 bp
  • A to C, chromosome 17 at 63,384,992 bp
  • G to T, chromosome 17 at 72,603,583 bp
  • A to T, chromosome 19 at 7,709,621 bp
  • T to A, chromosome 19 at 30,118,602 bp
  • A to G, chromosome 19 at 39,773,896 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0269 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
038495-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.