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Strain Name:
C57BL/6J-MtgxR0269Btlr/Mmmh
Stock Number:
038495-MU
Citation ID:
RRID:MMRRC_038495-MU
Other Names:
R0269 (G1), C57BL/6J-MtgxR0269Btlr
Major Collection:

Strain Information

Smarca4
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Synonyms: SNF2beta, Brg1, SW1/SNF, b2b692Clo, b2b508.1Clo
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20586
Homologene: 135927
Sema6d
Name: sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D
Synonyms: 1110067B02Rik, Sema6D-6, Sema6D-5, Sema6D-4, Sema6D-2, Sema6D-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 214968
Homologene: 16195
Gldc
Name: glycine decarboxylase
Synonyms: D19Wsu57e, D030049L12Rik, b2b2679Clo
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 104174
VEGA: 19
HGNC: HGNC:4313
Homologene: 141
Rgs7
Name: regulator of G protein signaling 7
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 24012
Homologene: 2193
Ntrk1
Name: neurotrophic tyrosine kinase, receptor, type 1
Synonyms: TrkA, Tkr
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18211
HGNC: HGNC:8031
Homologene: 1898
Hcn2
Name: hyperpolarization-activated, cyclic nucleotide-gated K+ 2
Synonyms: HAC1, trls
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 15166
HGNC: HGNC:4846
Homologene: 31022
Sgsm1
Name: small G protein signaling modulator 1
Synonyms: 2410098H20Rik, D5Bwg1524e, Rutbc2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 52850
Homologene: 64485
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 34,500,198 bp
  • A to G, chromosome 1 at 37,490,307 bp
  • A to G, chromosome 1 at 175,270,820 bp
  • T to C, chromosome 1 at 187,097,872 bp
  • T to A, chromosome 1 at 188,810,176 bp
  • T to G, chromosome 1 at 195,064,688 bp
  • A to G, chromosome 2 at 25,586,866 bp
  • A to C, chromosome 2 at 32,802,783 bp
  • T to C, chromosome 2 at 84,672,396 bp
  • A to G, chromosome 2 at 86,075,141 bp
  • A to G, chromosome 2 at 88,700,696 bp
  • T to A, chromosome 2 at 124,660,745 bp
  • A to G, chromosome 2 at 154,212,947 bp
  • C to T, chromosome 2 at 154,319,625 bp
  • T to C, chromosome 2 at 181,096,974 bp
  • T to C, chromosome 3 at 87,783,933 bp
  • T to C, chromosome 3 at 106,155,756 bp
  • T to C, chromosome 4 at 53,044,228 bp
  • A to T, chromosome 4 at 130,930,816 bp
  • A to G, chromosome 4 at 143,993,518 bp
  • A to T, chromosome 4 at 144,160,214 bp
  • T to C, chromosome 5 at 21,920,537 bp
  • A to C, chromosome 5 at 69,559,599 bp
  • A to T, chromosome 5 at 87,564,802 bp
  • T to C, chromosome 5 at 113,286,929 bp
  • A to G, chromosome 7 at 3,296,757 bp
  • T to A, chromosome 7 at 45,286,908 bp
  • T to A, chromosome 7 at 45,874,039 bp
  • T to C, chromosome 7 at 90,403,020 bp
  • T to C, chromosome 7 at 99,594,677 bp
  • G to T, chromosome 8 at 19,114,359 bp
  • A to G, chromosome 8 at 47,711,977 bp
  • T to C, chromosome 8 at 69,300,794 bp
  • T to G, chromosome 9 at 21,636,201 bp
  • T to C, chromosome 9 at 39,461,794 bp
  • T to C, chromosome 9 at 39,599,950 bp
  • T to A, chromosome 10 at 30,587,489 bp
  • A to G, chromosome 10 at 31,327,946 bp
  • A to G, chromosome 10 at 79,734,241 bp
  • T to C, chromosome 11 at 58,817,149 bp
  • T to A, chromosome 11 at 70,510,669 bp
  • C to A, chromosome 11 at 75,162,931 bp
  • T to C, chromosome 11 at 115,928,224 bp
  • T to A, chromosome 12 at 25,040,512 bp
  • C to A, chromosome 12 at 85,268,405 bp
  • T to C, chromosome 13 at 49,654,445 bp
  • A to T, chromosome 13 at 59,816,855 bp
  • A to T, chromosome 13 at 65,152,878 bp
  • T to A, chromosome 13 at 68,678,606 bp
  • G to T, chromosome 15 at 78,288,865 bp
  • C to T, chromosome 15 at 84,954,000 bp
  • T to C, chromosome 15 at 102,447,068 bp
  • A to T, chromosome 17 at 26,216,617 bp
  • A to T, chromosome 17 at 33,305,463 bp
  • A to C, chromosome 17 at 63,384,992 bp
  • G to T, chromosome 17 at 72,603,583 bp
  • A to T, chromosome 19 at 7,709,621 bp
  • T to A, chromosome 19 at 30,118,602 bp
  • A to G, chromosome 19 at 39,773,896 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0269 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038495-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.