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Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR0504Btlr/Mmmh
Stock Number:
038699-MU
Citation ID:
RRID:MMRRC_038699-MU
Other Names:
R0504 (G1), C57BL/6J-MtgxR0504Btlr
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Utrn
Name: utrophin
Synonyms: G-utrophin, Dmdl, DRP
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 22288
VEGA: 10
Homologene: 21398

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Thbs4
Name: thrombospondin 4
Synonyms: TSP-4, TSP4
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 21828
Homologene: 20691

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Nos2
Name: nitric oxide synthase 2, inducible
Synonyms: iNOS, Nos-2, NOS-II, Nos2a
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18126
HGNC: HGNC:7873
Homologene: 55473

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Get3
Name: guided entry of tail-anchored proteins factor 3, ATPase
Synonyms: ArsA, 1810048H22Rik, Asna1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 56495
HGNC: HGNC:752
Homologene: 31513

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Jmjd1c
Name: jumonji domain containing 1C
Synonyms: TRIP8, 5430433L24Rik, D630035I23Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 108829
Homologene: 3129

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IMPC
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BioGPS
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Kdm5b
Name: lysine demethylase 5B
Synonyms: PLU-1, 2010009J12Rik, Plu1, Rb-Bp2, 2210016I17Rik, D1Ertd202e, Jarid1b
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 75605
Homologene: 48448

Additional Resources
IMPC
Google
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IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
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ClinGen
Spidr
Name: scaffolding protein involved in DNA repair
Synonyms: 2310008H04Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224008
VEGA: 16
Homologene: 51693

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 40,329,056 bp
  • C to T, chromosome 1 at 88,088,357 bp
  • A to G, chromosome 1 at 119,765,915 bp
  • T to C, chromosome 1 at 134,324,113 bp
  • G to T, chromosome 1 at 134,621,023 bp
  • A to T, chromosome 1 at 135,968,529 bp
  • T to C, chromosome 1 at 138,088,697 bp
  • A to T, chromosome 1 at 150,876,419 bp
  • A to G, chromosome 1 at 163,386,605 bp
  • G to A, chromosome 1 at 166,491,545 bp
  • C to T, chromosome 1 at 171,217,236 bp
  • A to T, chromosome 1 at 175,687,182 bp
  • A to G, chromosome 1 at 179,545,831 bp
  • T to C, chromosome 2 at 18,684,072 bp
  • A to G, chromosome 2 at 54,085,055 bp
  • A to T, chromosome 2 at 59,878,325 bp
  • C to T, chromosome 2 at 66,222,798 bp
  • A to G, chromosome 2 at 76,749,536 bp
  • T to A, chromosome 2 at 85,904,686 bp
  • C to T, chromosome 2 at 89,296,739 bp
  • A to T, chromosome 2 at 89,618,094 bp
  • A to G, chromosome 2 at 92,613,824 bp
  • A to G, chromosome 2 at 121,302,941 bp
  • A to G, chromosome 2 at 130,615,917 bp
  • C to A, chromosome 2 at 156,052,195 bp
  • C to T, chromosome 2 at 167,424,205 bp
  • A to T, chromosome 3 at 64,389,363 bp
  • G to A, chromosome 3 at 70,027,604 bp
  • A to T, chromosome 3 at 87,813,156 bp
  • A to T, chromosome 3 at 94,643,133 bp
  • A to T, chromosome 3 at 116,786,784 bp
  • T to C, chromosome 4 at 32,698,916 bp
  • T to C, chromosome 4 at 43,263,559 bp
  • T to A, chromosome 4 at 82,912,637 bp
  • A to G, chromosome 4 at 103,270,860 bp
  • T to C, chromosome 4 at 118,372,952 bp
  • T to A, chromosome 4 at 123,116,840 bp
  • ACTG to A, chromosome 4 at 123,116,912 bp
  • C to A, chromosome 4 at 124,782,408 bp
  • C to A, chromosome 4 at 139,406,578 bp
  • T to C, chromosome 4 at 139,480,838 bp
  • T to A, chromosome 4 at 150,615,322 bp
  • A to G, chromosome 5 at 21,560,530 bp
  • A to G, chromosome 5 at 103,501,496 bp
  • A to G, chromosome 5 at 112,873,576 bp
  • T to A, chromosome 5 at 137,470,318 bp
  • G to A, chromosome 5 at 143,494,197 bp
  • A to T, chromosome 5 at 144,214,081 bp
  • A to T, chromosome 6 at 12,379,594 bp
  • T to A, chromosome 6 at 36,741,453 bp
  • C to T, chromosome 6 at 42,790,596 bp
  • A to T, chromosome 6 at 43,153,461 bp
  • C to T, chromosome 6 at 48,416,254 bp
  • A to T, chromosome 6 at 80,022,046 bp
  • A to G, chromosome 6 at 83,373,794 bp
  • T to A, chromosome 6 at 90,107,881 bp
  • T to A, chromosome 6 at 92,912,645 bp
  • A to T, chromosome 7 at 16,996,014 bp
  • T to C, chromosome 7 at 25,311,362 bp
  • T to A, chromosome 7 at 37,568,818 bp
  • T to G, chromosome 7 at 85,006,815 bp
  • C to T, chromosome 7 at 104,588,475 bp
  • T to A, chromosome 7 at 106,714,701 bp
  • C to T, chromosome 7 at 108,868,850 bp
  • T to A, chromosome 7 at 112,271,317 bp
  • C to T, chromosome 8 at 22,625,343 bp
  • T to A, chromosome 8 at 36,231,159 bp
  • A to C, chromosome 8 at 85,018,607 bp
  • A to T, chromosome 8 at 86,056,524 bp
  • T to G, chromosome 8 at 105,989,988 bp
  • T to C, chromosome 8 at 109,571,471 bp
  • T to C, chromosome 8 at 114,236,579 bp
  • A to T, chromosome 9 at 20,470,031 bp
  • A to G, chromosome 9 at 21,204,403 bp
  • G to T, chromosome 9 at 21,802,436 bp
  • G to T, chromosome 9 at 23,406,687 bp
  • A to G, chromosome 9 at 37,993,142 bp
  • T to C, chromosome 9 at 45,002,254 bp
  • A to G, chromosome 9 at 50,575,328 bp
  • A to G, chromosome 9 at 58,540,678 bp
  • G to A, chromosome 9 at 79,681,468 bp
  • C to A, chromosome 9 at 124,029,441 bp
  • A to G, chromosome 10 at 3,937,853 bp
  • A to T, chromosome 10 at 12,402,895 bp
  • T to C, chromosome 10 at 67,225,755 bp
  • T to C, chromosome 10 at 93,489,174 bp
  • A to C, chromosome 10 at 127,520,072 bp
  • A to T, chromosome 11 at 4,408,833 bp
  • T to A, chromosome 11 at 5,629,274 bp
  • T to G, chromosome 11 at 20,223,169 bp
  • T to C, chromosome 11 at 29,824,990 bp
  • A to G, chromosome 11 at 32,617,882 bp
  • C to A, chromosome 11 at 58,551,636 bp
  • A to G, chromosome 11 at 59,008,507 bp
  • T to G, chromosome 11 at 71,182,415 bp
  • T to A, chromosome 11 at 75,501,942 bp
  • C to T, chromosome 11 at 78,940,077 bp
  • A to G, chromosome 11 at 85,191,687 bp
  • G to T, chromosome 11 at 106,247,849 bp
  • T to A, chromosome 12 at 71,047,214 bp
  • A to T, chromosome 12 at 76,033,591 bp
  • A to T, chromosome 12 at 76,405,216 bp
  • T to A, chromosome 12 at 101,990,003 bp
  • T to A, chromosome 12 at 102,387,564 bp
  • T to C, chromosome 12 at 111,101,668 bp
  • A to G, chromosome 13 at 4,236,251 bp
  • C to G, chromosome 13 at 49,085,394 bp
  • T to A, chromosome 13 at 49,085,396 bp
  • A to C, chromosome 13 at 92,767,184 bp
  • T to C, chromosome 13 at 104,426,930 bp
  • T to A, chromosome 14 at 33,970,540 bp
  • A to G, chromosome 14 at 55,514,602 bp
  • T to C, chromosome 14 at 70,123,887 bp
  • A to G, chromosome 14 at 70,547,238 bp
  • T to A, chromosome 14 at 99,061,623 bp
  • T to C, chromosome 14 at 99,081,390 bp
  • T to A, chromosome 15 at 7,222,758 bp
  • T to C, chromosome 15 at 32,574,803 bp
  • T to C, chromosome 15 at 66,682,404 bp
  • A to C, chromosome 15 at 78,757,314 bp
  • T to A, chromosome 15 at 88,998,839 bp
  • A to G, chromosome 15 at 89,456,658 bp
  • A to C, chromosome 15 at 100,696,657 bp
  • A to G, chromosome 15 at 103,444,561 bp
  • C to T, chromosome 16 at 14,142,534 bp
  • A to C, chromosome 16 at 16,140,072 bp
  • A to G, chromosome 16 at 17,567,427 bp
  • A to G, chromosome 16 at 24,971,970 bp
  • A to C, chromosome 16 at 48,959,643 bp
  • A to G, chromosome 16 at 55,905,779 bp
  • A to G, chromosome 17 at 34,358,117 bp
  • A to G, chromosome 17 at 34,575,091 bp
  • T to A, chromosome 17 at 56,454,220 bp
  • A to G, chromosome 17 at 68,777,912 bp
  • T to A, chromosome 18 at 58,039,460 bp
  • G to A, chromosome 18 at 63,024,451 bp
  • A to T, chromosome 18 at 64,340,749 bp
  • C to T, chromosome 19 at 10,517,019 bp
  • T to A, chromosome 19 at 10,916,207 bp
  • T to A, chromosome 19 at 38,778,021 bp
  • T to A, chromosome 19 at 39,309,780 bp
  • C to A, chromosome 19 at 41,247,892 bp
  • T to A, chromosome 19 at 41,287,490 bp
  • T to C, chromosome 19 at 44,081,938 bp
  • G to T, chromosome 19 at 44,534,786 bp
  • T to C, chromosome 19 at 47,267,747 bp
Genotype Determination

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0504 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
038699-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.