Strain Name:
C57BL/6J-MtgxR0664Btlr/Mmmh
Stock Number:
038849-MU
Citation ID:
RRID:MMRRC_038849-MU
Other Names:
R0664 (G1), C57BL/6J-MtgxR0664Btlr
Major Collection:

Gene Information

Hlcs
Name: holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase)
Synonyms: 410I21.SP6, D16Jhu34
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 110948
VEGA: 16
HGNC: HGNC:4976
Homologene: 37302
Tanc1
Name: tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 1
Synonyms: 1200003E16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 66860
Homologene: 18946
Btbd11
Name: BTB (POZ) domain containing 11
Synonyms: 6330404E16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 74007
Homologene: 72536
Gpr55
Name: G protein-coupled receptor 55
Synonyms: LOC227326, CTFL
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227326
HGNC: HGNC:4511
Homologene: 36184
Thada
Name: thyroid adenoma associated
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 240174
VEGA: 17
Homologene: 75175
Epcam
Name: epithelial cell adhesion molecule
Synonyms: Ep-CAM, panepithelial glycoprotein 314, GA733-2, TROP1, Ly74, Egp314, EpCAM, gp40, CD326, EGP-2, Tacstd1, EpCAM1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 17075
VEGA: 17
Homologene: 1764
Mdn1
Name: midasin AAA ATPase 1
Synonyms: LOC213784, 4833432B22Rik, D4Abb1e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100019
Homologene: 39689
Ipo8
Name: importin 8
Synonyms: OM-1, 6230418K12Rik, Om1, C130009K11Rik, Ranbp8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 320727
HGNC: HGNC:9853
Homologene: 48430
Grid2ip
Name: glutamate receptor, ionotropic, delta 2 (Grid2) interacting protein 1
Synonyms: delphilin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 170935
Homologene: 15781
Jcad
Name: junctional cadherin 5 associated
Synonyms: 9430020K01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 240185
VEGA: 18
Homologene: 19370
Hgfac
Name: hepatocyte growth factor activator
Synonyms: HGFA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 54426
HGNC: HGNC:4894
Homologene: 1170
Prmt5
Name: protein arginine N-methyltransferase 5
Synonyms: Jbp1, Jak-binding protein 1, Skb1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 27374
Homologene: 4454
Ptpro
Name: protein tyrosine phosphatase, receptor type, O
Synonyms: D28, PTPROt, PTP-phi, PTP-BK, PTP-U2, GLEPP1, PTP-oc, Ptpn15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 19277
HGNC: HGNC:9678
Homologene: 21564
Cacna1b
Name: calcium channel, voltage-dependent, N type, alpha 1B subunit
Synonyms: Cchn1a, alpha(1B), Cav2.2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12287
HGNC: HGNC:1389
Homologene: 20184
Slco1a4
Name: solute carrier organic anion transporter family, member 1a4
Synonyms: Oatp2, Slc21a5, Oatp1a4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 28250
Homologene: 130782
Dnah7b
Name: dynein, axonemal, heavy chain 7B
Synonyms: LOC227058, Dnahc7b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227058
Homologene: 41287
Nsd3
Name: nuclear receptor binding SET domain protein 3
Synonyms: WHISTLE, Whsc1l1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234135
Homologene: 56960
A530064D06Rik
Name: RIKEN cDNA A530064D06 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 328830
VEGA: 17
Homologene: 136361
Ttbk2
Name: tau tubulin kinase 2
Synonyms: TTK, B930008N24Rik, 2610507N02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 140810
Homologene: 62795
Olfr1183
Name: olfactory receptor 1183
Synonyms: GA_x6K02T2Q125-49948903-49949814, MOR230-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258522
Homologene: 128125
4921517D22Rik
Name: RIKEN cDNA 4921517D22 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 70900
VEGA: 13
Homologene: 87278
Nfam1
Name: Nfat activating molecule with ITAM motif 1
Synonyms: Nfam1, 4921501M20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 74039
Homologene: 12528
Serpinb7
Name: serine (or cysteine) peptidase inhibitor, clade B, member 7
Synonyms: megsin, 4631416M05Rik, ovalbumin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 116872
Homologene: 68363
Champ1
Name: chromosome alignment maintaining phosphoprotein 1
Synonyms: D8Ertd569e, D8Ertd457e, Zfp828
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 101994
Homologene: 18780
Hsd17b2
Name: hydroxysteroid (17-beta) dehydrogenase 2
Synonyms: 17 HSD type 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 15486
HGNC: HGNC:5211
Homologene: 99709
Agmo
Name: alkylglycerol monooxygenase
Synonyms: A530016O06Rik, Tmem195
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 319660
Homologene: 45620
Emc9
Name: ER membrane protein complex subunit 9
Synonyms: Cgi112, 1500005A01Rik, Fam158a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 85308
Homologene: 41095
AC154303.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Stk26
Name: serine/threonine kinase 26
Synonyms: Mst4, 2610018G03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 70415
Homologene: 84402
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 46,324,842 bp
  • A to T, chromosome 1 at 85,941,017 bp
  • A to G, chromosome 1 at 107,428,307 bp
  • A to G, chromosome 2 at 24,654,446 bp
  • A to T, chromosome 2 at 59,843,884 bp
  • C to T, chromosome 2 at 88,462,171 bp
  • C to A, chromosome 2 at 120,748,821 bp
  • G to T, chromosome 4 at 32,768,011 bp
  • G to T, chromosome 5 at 35,048,178 bp
  • T to A, chromosome 5 at 143,363,977 bp
  • T to A, chromosome 6 at 137,443,594 bp
  • T to C, chromosome 6 at 141,812,741 bp
  • A to T, chromosome 6 at 148,800,213 bp
  • G to A, chromosome 8 at 13,879,485 bp
  • T to C, chromosome 8 at 25,714,240 bp
  • T to C, chromosome 8 at 117,758,701 bp
  • G to A, chromosome 10 at 85,388,370 bp
  • A to T, chromosome 12 at 37,252,572 bp
  • GCC to GC, chromosome 13 at 59,691,598 bp
  • G to T, chromosome 14 at 54,507,856 bp
  • A to G, chromosome 14 at 55,581,908 bp
  • G to A, chromosome 14 at 57,016,768 bp
  • T to C, chromosome 15 at 83,014,938 bp
  • A to G, chromosome 16 at 94,231,311 bp
  • T to C, chromosome 17 at 48,166,591 bp
  • A to G, chromosome 17 at 84,336,829 bp
  • T to A, chromosome 17 at 87,639,970 bp
  • A to G, chromosome 18 at 4,676,063 bp
  • T to A, chromosome X at 50,887,926 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0664 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
038849-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.