Strain Name:
C57BL/6J-MtgxR0710Btlr/Mmmh
Stock Number:
038893-MU
Citation ID:
RRID:MMRRC_038893-MU
Other Names:
R0710 (G1), C57BL/6J-MtgxR0710Btlr
Major Collection:

Gene Information

Hgf
Name: hepatocyte growth factor
Synonyms: scatter factor, NK1, SF/HGF, HGF/SF, NK2, C230052L06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 15234
HGNC: HGNC:4893
Homologene: 503
Scn3a
Name: sodium channel, voltage-gated, type III, alpha
Synonyms: LOC381367, Nav1.3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20269
Homologene: 56005
Tnpo3
Name: transportin 3
Synonyms: 5730544L10Rik, C430013M08Rik, D6Ertd313e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 320938
Homologene: 40848
Poli
Name: polymerase (DNA directed), iota
Synonyms: Rad30b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 26447
HGNC: HGNC:9182
Homologene: 5209
Fbxl3
Name: F-box and leucine-rich repeat protein 3
Synonyms: Fbl3a, Fbxl3a, Ovtm, Play68
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 50789
Homologene: 8127
Mtor
Name: mechanistic target of rapamycin kinase
Synonyms: FKBP-rapamycin-associated protein FRAP, 2610315D21Rik, RAPT1, RAFT1, flat, Frap1, mechanistic target of rapamycin (serine/threonine kinase)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 56717
HGNC: HGNC:3942
Homologene: 3637
Sinhcaf
Name: SIN3-HDAC complex associated factor
Synonyms: Tera, Pptcs1, Fam60a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 56306
Homologene: 10494
Ubr3
Name: ubiquitin protein ligase E3 component n-recognin 3
Synonyms: 4833421P10Rik, A130030D10Rik, 1110059H15Rik, Zfp650
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 68795
Homologene: 52092
Rasa3
Name: RAS p21 protein activator 3
Synonyms: GAPIII activator 3, Ras GTPase-activating protein III, GAPIII, R-Ras gap, scat, hlb381
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 19414
Homologene: 7217
Ubr2
Name: ubiquitin protein ligase E3 component n-recognin 2
Synonyms: 9930021A08Rik, E130209G04Rik, ENSMUSG00000043296
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224826
VEGA: 17
Homologene: 26151
Hmg20a
Name: high mobility group 20A
Synonyms: 1200004E06Rik, 5730490E10Rik, Hmgxb1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 66867
VEGA: 9
HGNC: HGNC:5001
Homologene: 32399
Hectd4
Name: HECT domain E3 ubiquitin protein ligase 4
Synonyms: Gm15800
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 269700
Homologene: 28297
Gpatch8
Name: G patch domain containing 8
Synonyms: 5430405G24Rik, Gpatc8, ENSMUSG00000075516, Fbm1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237943
Homologene: 46117
Krt74
Name: keratin 74
Synonyms: Kb37
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 406222
VEGA: 15
Kcnj11
Name: potassium inwardly rectifying channel, subfamily J, member 11
Synonyms: Kir6.2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 16514
HGNC: HGNC:6257
Homologene: 441
Iqch
Name: IQ motif containing H
Synonyms: 4921504K03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 78250
Homologene: 11258
Celsr2
Name: cadherin, EGF LAG seven-pass G-type receptor 2
Synonyms: flamingo, mfmi1, EGFL2, Adgrc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 53883
HGNC: HGNC:3231
Homologene: 1078
Sis
Name: sucrase isomaltase (alpha-glucosidase)
Synonyms: Si-s, sucrase-isomaltase, 2010204N08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 69983
Homologene: 37424
Plb1
Name: phospholipase B1
Synonyms: 4632413E21Rik, 4930433E17Rik, 4930539A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 665270
Homologene: 82108
Zfp282
Name: zinc finger protein 282
Synonyms: HUB1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 101095
Homologene: 2647
Farp1
Name: FERM, RhoGEF (Arhgef) and pleckstrin domain protein 1 (chondrocyte-derived)
Synonyms: Cdep
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 223254
HGNC: HGNC:3591
Homologene: 38098
Tulp2
Name: tubby-like protein 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 56734
Homologene: 2492
Wnk4
Name: WNK lysine deficient protein kinase 4
Synonyms: 2010002J11Rik, Prkwnk4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 69847
Homologene: 13020
Vmn1r171
Name: vomeronasal 1 receptor 171
Synonyms: V3R7, V1rd7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 81012
Homologene: 128341
Glra3
Name: glycine receptor, alpha 3 subunit
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 110304
HGNC: HGNC:4328
Homologene: 142
Kif1c
Name: kinesin family member 1C
Synonyms: N-3 kinsin, D11Bwg1349e, B430105J22Rik, Orch3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 16562
HGNC: HGNC:6317
Homologene: 4821
Mypop
Name: Myb-related transcription factor, partner of profilin
Synonyms: Myodysa, P42pop
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 232934
Homologene: 17083
Mlc1
Name: megalencephalic leukoencephalopathy with subcortical cysts 1 homolog (human)
Synonyms: Kiaa0027-hp, WKL1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 170790
Homologene: 15775
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 2 at 65,469,046 bp
  • T to C, chromosome 2 at 69,952,837 bp
  • T to C, chromosome 3 at 72,952,531 bp
  • A to G, chromosome 3 at 108,412,712 bp
  • T to C, chromosome 4 at 148,464,391 bp
  • C to A, chromosome 5 at 16,566,763 bp
  • T to A, chromosome 5 at 32,328,497 bp
  • G to A, chromosome 5 at 121,336,628 bp
  • T to C, chromosome 6 at 29,586,075 bp
  • C to T, chromosome 6 at 47,880,384 bp
  • A to G, chromosome 6 at 148,933,000 bp
  • A to T, chromosome 7 at 19,000,560 bp
  • C to A, chromosome 7 at 23,633,001 bp
  • T to A, chromosome 7 at 45,520,808 bp
  • T to C, chromosome 7 at 46,099,125 bp
  • C to A, chromosome 8 at 13,583,830 bp
  • C to T, chromosome 8 at 56,125,364 bp
  • G to A, chromosome 9 at 56,474,670 bp
  • A to T, chromosome 9 at 63,525,136 bp
  • A to G, chromosome 11 at 70,726,497 bp
  • G to A, chromosome 11 at 101,274,106 bp
  • A to G, chromosome 11 at 102,481,933 bp
  • G to T, chromosome 14 at 103,089,315 bp
  • C to T, chromosome 14 at 121,237,143 bp
  • G to T, chromosome 15 at 88,977,864 bp
  • T to A, chromosome 15 at 101,761,418 bp
  • G to A, chromosome 17 at 46,938,681 bp
  • A to T, chromosome 18 at 70,522,890 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0710 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
038893-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.