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Strain Name:
C57BL/6J-MtgxR1199Btlr/Mmmh
Stock Number:
039269-MU
Citation ID:
RRID:MMRRC_039269-MU
Other Names:
R1199 (G1), C57BL/6J-MtgxR1199Btlr
Major Collection:

Strain Information

Camk2a
Name: calcium/calmodulin-dependent protein kinase II alpha
Synonyms: alpha-CaMKII
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 12322
HGNC: HGNC:1460
Homologene: 56577
Vcan
Name: versican
Synonyms: PG-M, hdf, heart defect, 5430420N07Rik, Cspg2, DPEAAE
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 13003
HGNC: HGNC:2464
Homologene: 3228
Sez6
Name: seizure related gene 6
Synonyms: sez-6, D11Bhm177e
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20370
Homologene: 10948
Gpr37l1
Name: G protein-coupled receptor 37-like 1
Synonyms: CAG-18, D0Kist8
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 171469
Homologene: 3500
Pomt1
Name: protein-O-mannosyltransferase 1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 99011
HGNC: HGNC:9202
Homologene: 68548
Lpp
Name: LIM domain containing preferred translocation partner in lipoma
Synonyms: B130055L10Rik, 9430020K16Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 210126
VEGA: 16
HGNC: HGNC:6679
Homologene: 4075
Xrn1
Name: 5'-3' exoribonuclease 1
Synonyms: mXrn1, Dhm2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 24127
Homologene: 5894
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 82,289,626 bp
  • A to T, chromosome 1 at 135,166,972 bp
  • G to A, chromosome 1 at 188,759,795 bp
  • G to A, chromosome 2 at 28,665,626 bp
  • A to G, chromosome 2 at 32,250,492 bp
  • A to T, chromosome 2 at 37,961,716 bp
  • A to G, chromosome 2 at 76,908,756 bp
  • G to A, chromosome 2 at 76,950,044 bp
  • A to G, chromosome 2 at 85,918,035 bp
  • C to A, chromosome 2 at 120,673,636 bp
  • A to T, chromosome 2 at 157,109,461 bp
  • T to G, chromosome 3 at 19,977,152 bp
  • A to G, chromosome 3 at 88,420,986 bp
  • T to C, chromosome 4 at 59,914,189 bp
  • T to A, chromosome 4 at 111,799,145 bp
  • T to A, chromosome 4 at 156,172,299 bp
  • T to C, chromosome 5 at 24,751,148 bp
  • A to G, chromosome 5 at 43,979,597 bp
  • G to A, chromosome 5 at 89,215,794 bp
  • A to G, chromosome 5 at 134,411,064 bp
  • A to G, chromosome 5 at 134,933,616 bp
  • T to A, chromosome 6 at 30,449,494 bp
  • C to T, chromosome 6 at 41,688,591 bp
  • T to A, chromosome 6 at 91,986,978 bp
  • T to C, chromosome 6 at 106,353,597 bp
  • A to C, chromosome 7 at 5,544,972 bp
  • A to T, chromosome 7 at 103,777,985 bp
  • G to T, chromosome 8 at 48,235,582 bp
  • G to T, chromosome 8 at 125,887,314 bp
  • A to G, chromosome 9 at 95,981,761 bp
  • A to T, chromosome 9 at 108,393,477 bp
  • G to A, chromosome 10 at 76,579,819 bp
  • A to T, chromosome 10 at 86,657,325 bp
  • T to A, chromosome 10 at 130,448,574 bp
  • G to A, chromosome 11 at 29,755,044 bp
  • A to T, chromosome 11 at 49,180,747 bp
  • A to G, chromosome 11 at 70,708,601 bp
  • A to G, chromosome 11 at 77,953,885 bp
  • T to C, chromosome 12 at 52,796,190 bp
  • G to A, chromosome 13 at 19,142,028 bp
  • A to T, chromosome 13 at 22,102,658 bp
  • A to T, chromosome 13 at 75,096,413 bp
  • A to G, chromosome 13 at 89,679,794 bp
  • T to C, chromosome 15 at 55,252,010 bp
  • A to T, chromosome 15 at 60,919,635 bp
  • C to T, chromosome 15 at 76,854,236 bp
  • C to T, chromosome 15 at 81,895,193 bp
  • G to A, chromosome 15 at 89,419,010 bp
  • C to T, chromosome 16 at 24,681,860 bp
  • C to T, chromosome 16 at 34,723,828 bp
  • C to A, chromosome 16 at 91,618,642 bp
  • A to G, chromosome 17 at 20,583,263 bp
  • A to T, chromosome 17 at 23,817,751 bp
  • T to A, chromosome 18 at 5,059,217 bp
  • T to C, chromosome 18 at 34,438,216 bp
  • T to A, chromosome 18 at 60,952,324 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1199 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039269-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.