Strain Name:
C57BL/6J-MtgxR1328Btlr/Mmmh
Stock Number:
039394-MU
Citation ID:
RRID:MMRRC_039394-MU
Other Names:
R1328 (G1), C57BL/6J-MtgxR1328Btlr
Major Collection:

Strain Information

Polr3e
Name: polymerase (RNA) III (DNA directed) polypeptide E
Synonyms: RPC5, Sin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 26939
Homologene: 14052
Bcap29
Name: B cell receptor associated protein 29
Synonyms: Bap29
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 12033
VEGA: 12
Homologene: 22411
Fip1l1
Name: FIP1 like 1 (S. cerevisiae)
Synonyms: Rje, 1300019H17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 66899
Homologene: 136254
Copa
Name: coatomer protein complex subunit alpha
Synonyms: xenin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12847
HGNC: HGNC:2230
Homologene: 3218
Rrp36
Name: ribosomal RNA processing 36
Synonyms: BC011248
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224823
VEGA: 17
Homologene: 40346
Dmxl2
Name: Dmx-like 2
Synonyms: E130119P06Rik, 6430411K14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235380
HGNC: HGNC:2938
Homologene: 41022
Trim33
Name: tripartite motif-containing 33
Synonyms: Tif1g, 8030451N04Rik, ectodermin, Ecto
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 94093
Homologene: 9296
Prmt9
Name: protein arginine methyltransferase 9
Synonyms: Prmt10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 102182
Homologene: 16298
Sag
Name: S-antigen, retina and pineal gland (arrestin)
Synonyms: A930001K18Rik, rod arrestin, Arr1, arrestin 1, visual arrestin 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 20215
Homologene: 455
Pou4f2
Name: POU domain, class 4, transcription factor 2
Synonyms: Brn-3.2, Brn-3b, mBrn3-3R, Brn3b, Pou4f-rs1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 18997
HGNC: HGNC:9219
Homologene: 20959
Stxbp2
Name: syntaxin binding protein 2
Synonyms: Munc-18b, Munc-18-2, C79054, Sxtp2, Munc18b, muSec1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 20911
Homologene: 55530
Pkhd1l1
Name: polycystic kidney and hepatic disease 1-like 1
Synonyms: D86 mRNA, PKHDL1, fibrocystin L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 192190
Homologene: 16332
Rin2
Name: Ras and Rab interactor 2
Synonyms: RASSF4, 4632403N06Rik, 2010003K16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 74030
Homologene: 32430
Il23r
Name: interleukin 23 receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 209590
Homologene: 16930
Or6c202
Name: olfactory receptor family 6 subfamily C member 202
Synonyms: GA_x6K02T2PULF-10846420-10845467, MOR114-8, Olfr771
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 258540
Homologene: 74047
Ccnb1ip1
Name: cyclin B1 interacting protein 1
Synonyms: LOC239083, mei4, Hei10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 239083
VEGA: 14
Homologene: 18819
Krt18
Name: keratin 18
Synonyms: K18, Endo B, Krt1-18, CK18
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 16668
VEGA: 15
HGNC: HGNC:6430
Homologene: 55448
Flnc
Name: filamin C, gamma
Synonyms: Fln2, 1110055E19Rik, actin binding protein 280
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 68794
HGNC: HGNC:3756
Homologene: 37481
Or9i14
Name: olfactory receptor family 9 subfamily I member 14
Synonyms: GA_x6K02T2RE5P-4147744-4146800, MOR211-2, Olfr1499
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 258792
Homologene: 17393
Tbc1d31
Name: TBC1 domain family, member 31
Synonyms: LOC210544, D330013L20Rik, Wdr67
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 210544
Homologene: 17089
Mast1
Name: microtubule associated serine/threonine kinase 1
Synonyms: 9430008B02Rik, SAST170, SAST
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 56527
Homologene: 10543
Vmn2r118
Name: vomeronasal 2, receptor 118
Synonyms: EG668547, Vmn2r119, EG383258
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 383258
Homologene: 129687
Setd7
Name: SET domain containing (lysine methyltransferase) 7
Synonyms: 1600028F23Rik, Set7, KMT7, Set7/9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 73251
Homologene: 12741
Fbxl14
Name: F-box and leucine-rich repeat protein 14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 101358
Homologene: 15271
Gm9934
Name: predicted gene 9934
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Sox13
Name: SRY (sex determining region Y)-box 13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 20668
Homologene: 4159
Vmn1r8
Name: vomeronasal 1 receptor 8
Synonyms: V1rc32
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 171205
Homologene: 83427
Acp4
Name: acid phosphatase 4
Synonyms: EG546967, Acpt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100503991
Homologene: 57200
Smr2
Name: submaxillary gland androgen regulated protein 2
Synonyms: MSG2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 20600
Srd5a1
Name: steroid 5 alpha-reductase 1
Synonyms: Srd5a-1, 4930435F02Rik, 0610031P22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 78925
Homologene: 37426
H2-M3
Name: histocompatibility 2, M region locus 3
Synonyms: Hmt, H-2M3, R4B2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 14991
Homologene: 133255
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 87,810,294 bp
  • T to C, chromosome 1 at 133,383,817 bp
  • C to T, chromosome 1 at 172,121,691 bp
  • C to T, chromosome 2 at 145,860,446 bp
  • T to C, chromosome 3 at 51,542,819 bp
  • A to G, chromosome 3 at 103,353,597 bp
  • A to G, chromosome 4 at 144,271,488 bp
  • T to A, chromosome 5 at 74,546,135 bp
  • AT to ATT, chromosome 5 at 88,108,824 bp
  • T to C, chromosome 6 at 29,438,613 bp
  • T to C, chromosome 6 at 57,036,293 bp
  • T to A, chromosome 6 at 67,491,818 bp
  • T to C, chromosome 6 at 119,480,386 bp
  • C to T, chromosome 7 at 44,257,092 bp
  • T to C, chromosome 7 at 93,080,229 bp
  • C to T, chromosome 7 at 120,933,823 bp
  • A to T, chromosome 8 at 3,642,657 bp
  • T to C, chromosome 8 at 77,572,654 bp
  • G to A, chromosome 8 at 78,436,130 bp
  • A to G, chromosome 8 at 84,917,988 bp
  • G to T, chromosome 9 at 54,396,249 bp
  • A to G, chromosome 10 at 129,160,424 bp
  • T to C, chromosome 12 at 31,630,808 bp
  • T to A, chromosome 13 at 69,575,191 bp
  • A to T, chromosome 14 at 50,789,925 bp
  • T to C, chromosome 15 at 44,497,996 bp
  • T to C, chromosome 15 at 57,942,463 bp
  • C to T, chromosome 15 at 102,030,734 bp
  • T to C, chromosome 17 at 37,271,034 bp
  • T to A, chromosome 17 at 46,672,779 bp
  • C to A, chromosome 17 at 55,608,620 bp
  • G to A, chromosome 19 at 13,815,536 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1328 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039394-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.