Strain Name:
C57BL/6J-MtgxR1566Btlr/Mmmh
Stock Number:
039605-MU
Citation ID:
RRID:MMRRC_039605-MU
Other Names:
R1566 (G1), C57BL/6J-MtgxR1566Btlr
Major Collection:

Gene Information

Gene Symbol: Pkd1 [MGI:97603] (Mus musculus (mouse))
Name: polycystin 1, transient receptor poteintial channel interacting
Synonyms: PC1, PC-1, polycystin-1
Chromosome: 17
Alteration at locus: Chemically Induced
Gene Symbol: Arhgef7 [MGI:1860493] (Mus musculus (mouse))
Name: Rho guanine nucleotide exchange factor (GEF7)
Synonyms: cool-1, betaPix-b, Pak interacting exchange factor, betaPix, Cool, betaPix-c, PIX, p85SPR
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Pank4 [MGI:2387466] (Mus musculus (mouse))
Name: pantothenate kinase 4
Synonyms: D030031I12Rik
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Epb41l1 [MGI:103010] (Mus musculus (mouse))
Name: erythrocyte membrane protein band 4.1 like 1
Synonyms: 4.1N, Epb4.1l1
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Gdnf [MGI:107430] (Mus musculus (mouse))
Name: glial cell line derived neurotrophic factor
Synonyms: glial cell line-derived neurotrophic factor
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Pcm1 [MGI:1277958] (Mus musculus (mouse))
Name: pericentriolar material 1
Synonyms: C030044G17Rik, 2600002H09Rik, 9430077F19Rik
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Usp38 [MGI:1922091] (Mus musculus (mouse))
Name: ubiquitin specific peptidase 38
Synonyms: 4833420O05Rik, 4631402N15Rik
Chromosome: 8
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 46,836,085 bp
  • A to G, chromosome 1 at 65,388,538 bp
  • T to C, chromosome 1 at 132,059,179 bp
  • C to A, chromosome 1 at 174,184,706 bp
  • G to A, chromosome 2 at 59,876,715 bp
  • A to C, chromosome 2 at 77,415,913 bp
  • T to A, chromosome 2 at 83,736,630 bp
  • G to A, chromosome 2 at 86,314,785 bp
  • A to T, chromosome 2 at 120,502,993 bp
  • A to G, chromosome 2 at 156,521,959 bp
  • A to G, chromosome 2 at 180,278,188 bp
  • G to A, chromosome 3 at 14,650,698 bp
  • T to A, chromosome 3 at 40,692,578 bp
  • T to C, chromosome 3 at 125,875,558 bp
  • A to C, chromosome 4 at 126,911,147 bp
  • C to T, chromosome 4 at 154,883,653 bp
  • T to C, chromosome 4 at 154,980,521 bp
  • C to A, chromosome 5 at 34,000,856 bp
  • G to A, chromosome 5 at 111,225,677 bp
  • G to T, chromosome 6 at 4,523,613 bp
  • G to A, chromosome 6 at 40,999,409 bp
  • T to C, chromosome 6 at 42,291,440 bp
  • G to A, chromosome 6 at 48,466,870 bp
  • C to T, chromosome 6 at 56,736,482 bp
  • A to G, chromosome 6 at 130,231,601 bp
  • G to C, chromosome 6 at 130,333,144 bp
  • A to G, chromosome 7 at 3,822,936 bp
  • T to A, chromosome 7 at 29,092,175 bp
  • A to G, chromosome 7 at 30,609,720 bp
  • T to C, chromosome 7 at 44,903,480 bp
  • T to C, chromosome 7 at 45,340,688 bp
  • G to A, chromosome 7 at 47,188,904 bp
  • T to G, chromosome 7 at 107,097,552 bp
  • C to A, chromosome 8 at 9,973,650 bp
  • A to G, chromosome 8 at 11,782,620 bp
  • C to T, chromosome 8 at 22,803,951 bp
  • T to A, chromosome 8 at 41,290,773 bp
  • T to C, chromosome 8 at 80,984,803 bp
  • C to T, chromosome 8 at 111,997,658 bp
  • T to C, chromosome 9 at 26,880,870 bp
  • T to A, chromosome 9 at 45,244,149 bp
  • A to T, chromosome 9 at 78,242,459 bp
  • G to A, chromosome 9 at 108,125,985 bp
  • C to T, chromosome 10 at 62,949,187 bp
  • A to T, chromosome 11 at 72,058,959 bp
  • T to A, chromosome 11 at 109,788,806 bp
  • A to G, chromosome 11 at 117,769,436 bp
  • T to C, chromosome 12 at 16,711,828 bp
  • T to A, chromosome 12 at 40,081,674 bp
  • A to T, chromosome 12 at 70,054,479 bp
  • T to C, chromosome 12 at 76,572,065 bp
  • T to A, chromosome 12 at 83,675,054 bp
  • A to T, chromosome 12 at 87,517,231 bp
  • T to A, chromosome 12 at 108,471,892 bp
  • C to T, chromosome 12 at 111,824,848 bp
  • T to A, chromosome 13 at 27,315,427 bp
  • T to C, chromosome 13 at 33,253,494 bp
  • A to C, chromosome 13 at 55,412,031 bp
  • G to A, chromosome 14 at 52,176,655 bp
  • A to G, chromosome 14 at 57,441,011 bp
  • T to A, chromosome 14 at 105,160,544 bp
  • A to G, chromosome 15 at 7,834,414 bp
  • T to C, chromosome 15 at 81,768,834 bp
  • T to C, chromosome 15 at 102,093,728 bp
  • T to A, chromosome 16 at 19,286,327 bp
  • T to C, chromosome 16 at 26,963,939 bp
  • T to A, chromosome 16 at 58,889,540 bp
  • A to G, chromosome 16 at 89,419,738 bp
  • T to C, chromosome 17 at 24,591,000 bp
  • T to A, chromosome 17 at 33,136,974 bp
  • T to A, chromosome 17 at 34,189,546 bp
  • T to C, chromosome 17 at 57,193,876 bp
  • A to G, chromosome 17 at 80,453,916 bp
  • T to C, chromosome 18 at 61,755,643 bp
  • T to C, chromosome 18 at 68,250,598 bp
  • C to A, chromosome 19 at 5,465,417 bp
  • T to A, chromosome 19 at 8,742,311 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1566 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039605-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.