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Strain Name:
C57BL/6J-MtgxR1628Btlr/Mmmh
Stock Number:
039665-MU
Citation ID:
RRID:MMRRC_039665-MU
Other Names:
R1628 (G1), C57BL/6J-MtgxR1628Btlr
Major Collection:

Strain Information

Gpr6
Name: G protein-coupled receptor 6
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 140741
VEGA: 10
HGNC: HGNC:4515
Homologene: 38026
Etv5
Name: ets variant 5
Synonyms: 8430401F14Rik, erm, 1110005E01Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 104156
HGNC: HGNC:3494
Homologene: 3276
Pecam1
Name: platelet/endothelial cell adhesion molecule 1
Synonyms: PECAM-1, Cd31
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18613
HGNC: HGNC:8823
Homologene: 47925
B3galnt1
Name: UDP-GalNAc:betaGlcNAc beta 1,3-galactosaminyltransferase, polypeptide 1
Synonyms: Mbrn 1, Brainiac 1, Globoside blood group, B3galt3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 26879
HGNC: HGNC:918
Homologene: 32457
Dapk3
Name: death-associated protein kinase 3
Synonyms: ZIP kinase
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 13144
VEGA: 10
HGNC: HGNC:2676
Homologene: 20353
Rapgef6
Name: Rap guanine nucleotide exchange factor (GEF) 6
Synonyms: PDZ-GEF2, RA-GEF-2, Pdzgef2, C030018K18Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 192786
Homologene: 22968
Wwox
Name: WW domain-containing oxidoreductase
Synonyms: WOX1, 9030416C10Rik, 5330426P09Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 80707
Homologene: 56334
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 52,232,676 bp
  • A to C, chromosome 1 at 74,571,888 bp
  • T to C, chromosome 1 at 107,532,551 bp
  • T to A, chromosome 1 at 136,171,220 bp
  • T to A, chromosome 1 at 171,704,852 bp
  • T to C, chromosome 2 at 5,311,037 bp
  • A to G, chromosome 2 at 37,004,726 bp
  • A to G, chromosome 2 at 70,286,962 bp
  • A to C, chromosome 2 at 130,687,127 bp
  • A to G, chromosome 2 at 158,430,463 bp
  • G to A, chromosome 3 at 69,575,628 bp
  • C to T, chromosome 3 at 87,089,151 bp
  • A to T, chromosome 3 at 117,328,272 bp
  • A to G, chromosome 4 at 33,152,432 bp
  • G to T, chromosome 4 at 43,263,371 bp
  • C to T, chromosome 4 at 43,570,118 bp
  • C to T, chromosome 4 at 58,107,561 bp
  • A to G, chromosome 4 at 116,997,392 bp
  • A to G, chromosome 4 at 123,208,491 bp
  • T to A, chromosome 5 at 41,816,982 bp
  • G to A, chromosome 5 at 65,803,572 bp
  • T to C, chromosome 5 at 109,219,972 bp
  • T to A, chromosome 5 at 114,342,094 bp
  • A to G, chromosome 5 at 143,717,367 bp
  • A to T, chromosome 5 at 146,001,463 bp
  • A to T, chromosome 6 at 3,700,251 bp
  • T to G, chromosome 6 at 30,444,462 bp
  • A to T, chromosome 6 at 41,357,456 bp
  • A to G, chromosome 6 at 67,423,609 bp
  • A to G, chromosome 6 at 112,739,370 bp
  • A to T, chromosome 6 at 125,647,738 bp
  • G to T, chromosome 6 at 142,102,966 bp
  • A to T, chromosome 7 at 18,180,307 bp
  • A to G, chromosome 7 at 19,223,199 bp
  • A to G, chromosome 7 at 24,274,857 bp
  • G to A, chromosome 7 at 42,136,406 bp
  • A to G, chromosome 7 at 43,954,141 bp
  • A to G, chromosome 7 at 72,211,589 bp
  • T to C, chromosome 7 at 104,840,841 bp
  • A to T, chromosome 7 at 106,603,269 bp
  • G to A, chromosome 7 at 127,092,583 bp
  • T to A, chromosome 8 at 84,978,410 bp
  • A to G, chromosome 8 at 114,448,233 bp
  • A to G, chromosome 8 at 126,430,860 bp
  • T to G, chromosome 10 at 38,821,534 bp
  • C to T, chromosome 10 at 41,071,548 bp
  • C to T, chromosome 10 at 72,656,295 bp
  • C to A, chromosome 10 at 81,191,809 bp
  • T to C, chromosome 10 at 81,334,507 bp
  • T to G, chromosome 10 at 121,870,339 bp
  • A to G, chromosome 11 at 23,488,725 bp
  • TG to TGG, chromosome 11 at 54,546,397 bp
  • A to C, chromosome 11 at 68,944,814 bp
  • T to A, chromosome 11 at 82,929,380 bp
  • A to T, chromosome 11 at 106,682,960 bp
  • A to C, chromosome 12 at 4,629,652 bp
  • A to G, chromosome 12 at 76,583,848 bp
  • A to G, chromosome 12 at 83,784,406 bp
  • G to A, chromosome 12 at 98,982,521 bp
  • C to G, chromosome 13 at 15,726,312 bp
  • G to A, chromosome 13 at 33,093,654 bp
  • T to C, chromosome 13 at 50,702,288 bp
  • A to G, chromosome 13 at 76,111,791 bp
  • A to G, chromosome 13 at 120,026,878 bp
  • A to T, chromosome 14 at 32,959,961 bp
  • T to A, chromosome 15 at 9,182,506 bp
  • A to G, chromosome 15 at 81,360,724 bp
  • A to G, chromosome 15 at 99,100,936 bp
  • C to T, chromosome 16 at 22,401,671 bp
  • C to T, chromosome 17 at 84,691,991 bp
  • T to C, chromosome 18 at 12,734,879 bp
  • G to A, chromosome 18 at 84,354,533 bp
  • G to T, chromosome 19 at 5,686,065 bp
  • A to G, chromosome 19 at 6,230,905 bp
  • G to A, chromosome X at 139,837,845 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1628 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039665-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.