Strain Name:
C57BL/6J-MtgxR1657Btlr/Mmmh
Stock Number:
039693-MU
Citation ID:
RRID:MMRRC_039693-MU
Other Names:
R1657 (G1), C57BL/6J-MtgxR1657Btlr
Major Collection:

Gene Information

H2-DMa
Name: histocompatibility 2, class II, locus DMa
Synonyms: H2-Ma, H-2Ma, H2-M alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 14998
HGNC: HGNC:4934
Homologene: 4464
Vldlr
Name: very low density lipoprotein receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 22359
Homologene: 443
Snx17
Name: sorting nexin 17
Synonyms: D5Ertd260e, 5830447M19Rik, b2b1625.1Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 266781
Homologene: 8838
Cfl1
Name: cofilin 1, non-muscle
Synonyms: cofilin, Cof, n-cofilin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 12631
HGNC: HGNC:1874
Homologene: 99735
Morf4l1
Name: mortality factor 4 like 1
Synonyms: TEG-189, MRG15, Tex189, MORFRG15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 21761
Homologene: 86043
Itsn1
Name: intersectin 1 (SH3 domain protein 1A)
Synonyms: Ese1, Sh3p17, EHSH1, Eh domain, SH3 domain regulator of endocytosis 1, Intersectin-L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 16443
HGNC: HGNC:6183
Homologene: 2277
Nln
Name: neurolysin (metallopeptidase M3 family)
Synonyms: 4930472G13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 75805
VEGA: 13
Homologene: 69315
Rpap1
Name: RNA polymerase II associated protein 1
Synonyms: A730023M06Rik, 1190005L06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 68925
Homologene: 32269
Fdft1
Name: farnesyl diphosphate farnesyl transferase 1
Synonyms: squalene synthase, Ss, SQS
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 14137
VEGA: 14
HGNC: HGNC:3629
Homologene: 3281
Zfp341
Name: zinc finger protein 341
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228807
Homologene: 13127
Caprin1
Name: cell cycle associated protein 1
Synonyms: caprin-1, MMGPIP137, RNG105, Gpiap1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 53872
HGNC: HGNC:6743
Homologene: 4310
Polr1a
Name: polymerase (RNA) I polypeptide A
Synonyms: mRPA1, 3010014K16Rik, Rpo1-4, 194kDa, RPA194, 2900087K15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 20019
Homologene: 7033
Als2
Name: alsin Rho guanine nucleotide exchange factor
Synonyms: 3222402C23Rik, 9430073A21Rik, Alsin, Als2cr6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 74018
HGNC: HGNC:443
Homologene: 23264
Zfp985
Name: zinc finger protein 985
Synonyms: Gm13154
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 433804
Homologene: 133076
Chd2
Name: chromodomain helicase DNA binding protein 2
Synonyms: 5630401D06Rik, 2810013C04Rik, 2810040A01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 244059
HGNC: HGNC:1917
Homologene: 37462
Acaca
Name: acetyl-Coenzyme A carboxylase alpha
Synonyms: Acc1, LOC327983, A530025K05Rik, acetyl-CoA carboxylase, Acac
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 107476
HGNC: HGNC:84
Homologene: 31015
Esam
Name: endothelial cell-specific adhesion molecule
Synonyms: W117m, 2310008D05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 69524
Homologene: 12316
Mfge8
Name: milk fat globule-EGF factor 8 protein
Synonyms: lactadherin, SED1, EGF/factor VIII, MFG-E8, Mfgm
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 17304
HGNC: HGNC:7036
Homologene: 4334
Snx9
Name: sorting nexin 9
Synonyms: SH3PX1, SDP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 66616
VEGA: 17
Homologene: 49454
Zfp746
Name: zinc finger protein 746
Synonyms: 2810407L07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 69228
Homologene: 19783
Dhx29
Name: DEAH (Asp-Glu-Ala-His) box polypeptide 29
Synonyms: E130202M19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218629
VEGA: 13
Homologene: 10387
Dact2
Name: dishevelled-binding antagonist of beta-catenin 2
Synonyms: 2900084M21Rik, dapper2, Dpr2, Frd2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 240025
VEGA: 17
Homologene: 18237
Armh4
Name: armadillo-like helical domain containing 4
Synonyms: 3632451O06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 67419
Homologene: 12127
0610009B22Rik
Name: RIKEN cDNA 0610009B22 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 66050
Homologene: 133292
Col9a2
Name: collagen, type IX, alpha 2
Synonyms: Col9a-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 12840
HGNC: HGNC:2218
Homologene: 37535
Ttn
Name: titin
Synonyms: D330041I19Rik, mdm, L56, connectin, 2310074I15Rik, 2310057K23Rik, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, shru
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Lrp1
Name: low density lipoprotein receptor-related protein 1
Synonyms: CD91, A2mr, b2b1554Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 16971
HGNC: HGNC:6692
Homologene: 1744
Pld1
Name: phospholipase D1
Synonyms: Pld1a, Pld1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 18805
HGNC: HGNC:9067
Homologene: 116234
Kmt5c
Name: lysine methyltransferase 5C
Synonyms: Suv4-20h2, Suv420h2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 232811
Homologene: 32792
Mroh2b
Name: maestro heat-like repeat family member 2B
Synonyms: Heatr7b2, 4930455B06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223825
VEGA: 15
Homologene: 128807
Scn5a
Name: sodium channel, voltage-gated, type V, alpha
Synonyms: mH1, Nav1.5, Nav1.5c, SkM2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 20271
Homologene: 22738
Fer1l4
Name: fer-1-like 4 (C. elegans)
Synonyms: 9130402C12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 74562
Homologene: 19075
Kif9
Name: kinesin family member 9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 16578
Homologene: 65620
Hsd3b5
Name: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 5
Synonyms: 3(beta)HSDV
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 15496
Homologene: 104115
Peli2
Name: pellino 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 93834
VEGA: 14
HGNC: HGNC:8828
Homologene: 41431
Sema3d
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D
Synonyms: 4631426B19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 108151
Homologene: 23664
Cgnl1
Name: cingulin-like 1
Synonyms: 4933421H10Rik, 9930020M10Rik, Jacop
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 68178
Homologene: 41901
Tmem266
Name: transmembrane protein 266
Synonyms: AI118078
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 244886
Homologene: 17551
Celsr3
Name: cadherin, EGF LAG seven-pass G-type receptor 3
Synonyms: Adgrc3, Fmi1, flamingo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 107934
HGNC: HGNC:3230
Homologene: 1077
Snap47
Name: synaptosomal-associated protein, 47
Synonyms: SNAP-47, 1110031B06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 67826
Homologene: 14206
Aox4
Name: aldehyde oxidase 4
Synonyms: 2310003G12Rik, AOH2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 71872
Homologene: 70273
Grk3
Name: G protein-coupled receptor kinase 3
Synonyms: 4833444A01Rik, Adrbk2, Adrbk-2, Bark-2, beta ARK2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 320129
HGNC: HGNC:290
Homologene: 21072
Itgav
Name: integrin alpha V
Synonyms: alphav-integrin, D430040G12Rik, 2610028E01Rik, 1110004F14Rik, vitronectin receptor alpha polypeptide (VNRA), CD51
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16410
HGNC: HGNC:6150
Homologene: 20510
Serpinb6c
Name: serine (or cysteine) peptidase inhibitor, clade B, member 6c
Synonyms: SPIC, Spi3C, ovalbumin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 97848
HGNC: HGNC:8950
Inpp5e
Name: inositol polyphosphate-5-phosphatase E
Synonyms: 72kDa, 1200002L24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 64436
Homologene: 56863
Zfp455
Name: zinc finger protein 455
Synonyms: 3732412P20Rik, Rslcan-10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218311
Homologene: 110878
Olfr1065
Name: olfactory receptor 1065
Synonyms: MOR190-1, GA_x6K02T2Q125-47915274-47914333
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258403
Homologene: 74195
Kcnh8
Name: potassium voltage-gated channel, subfamily H (eag-related), member 8
Synonyms: C130090D05Rik, Kv12.1, ELK1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 211468
Homologene: 14332
Fam189a2
Name: family with sequence similarity 189, member A2
Synonyms: LOC381217
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 381217
VEGA: 19
Homologene: 3540
Sphkap
Name: SPHK1 interactor, AKAP domain containing
Synonyms: 4930544G21Rik, SKIP, A930009L15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 77629
Homologene: 18172
Ocstamp
Name: osteoclast stimulatory transmembrane protein
Synonyms: 4833422F24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 74614
Homologene: 41768
Nipsnap1
Name: nipsnap homolog 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18082
HGNC: HGNC:7827
Homologene: 2695
Zfp184
Name: zinc finger protein 184 (Kruppel-like)
Synonyms: 4930500C15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 193452
Homologene: 113602
Mtif2
Name: mitochondrial translational initiation factor 2
Synonyms: 2410112O06Rik, 2310038D14Rik, IF-2mt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 76784
HGNC: HGNC:7441
Homologene: 1840
Cyp3a44
Name: cytochrome P450, family 3, subfamily a, polypeptide 44
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 337924
HGNC: HGNC:2638
Homologene: 133568
Tubal3
Name: tubulin, alpha-like 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 238463
VEGA: 13
Homologene: 23488
Cbr4
Name: carbonyl reductase 4
Synonyms: A730083J17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234309
Homologene: 57181
Olfr503
Name: olfactory receptor 503
Synonyms: MOR34-8P, MOR34-12, MOR34-9, GA_x6K02T2PBJ9-10874315-10875286, Olfr1548, MOR34-9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 259143
Homologene: 121509
Zeb2
Name: zinc finger E-box binding homeobox 2
Synonyms: 9130203F04Rik, Zfhx1b, Zfx1b, SIP1, D130016B08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 24136
Homologene: 8868
Qsox2
Name: quiescin Q6 sulfhydryl oxidase 2
Synonyms: Qscn6l1, QSOX2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227638
Homologene: 65608
Rpe65
Name: retinal pigment epithelium 65
Synonyms: rd12, Mord1, A930029L06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 19892
Homologene: 20108
Olfr403
Name: olfactory receptor 403
Synonyms: IA7, MOR125-5_p, GA_x6K02T2P1NL-4348188-4349129
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 404316
HGNC: HGNC:8179
Homologene: 8219
Amdhd2
Name: amidohydrolase domain containing 2
Synonyms: 5730457F11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 245847
Homologene: 9311
Terb1
Name: telomere repeat binding bouquet formation protein 1
Synonyms: 4930532D21Rik, Ccdc79
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 320022
Homologene: 18330
Slc26a10
Name: solute carrier family 26, member 10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216441
VEGA: 10
Homologene: 66953
Zc3h8
Name: zinc finger CCCH type containing 8
Synonyms: Fliz1, E130108N08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 57432
Homologene: 10759
Nr2e3
Name: nuclear receptor subfamily 2, group E, member 3
Synonyms: photoreceptor-specific nuclear receptor, Pnr, RNR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 23958
HGNC: HGNC:7974
Homologene: 84397
Lcn9
Name: lipocalin 9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 77704
Homologene: 12681
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 58,266,810 bp
  • A to G, chromosome 1 at 59,180,601 bp
  • G to A, chromosome 1 at 83,277,515 bp
  • G to A, chromosome 2 at 25,824,710 bp
  • A to T, chromosome 2 at 26,220,747 bp
  • C to T, chromosome 2 at 26,399,582 bp
  • A to C, chromosome 2 at 44,994,465 bp
  • T to C, chromosome 2 at 76,742,804 bp
  • A to T, chromosome 2 at 83,801,779 bp
  • A to C, chromosome 2 at 86,445,218 bp
  • A to T, chromosome 2 at 103,769,506 bp
  • T to C, chromosome 2 at 119,783,778 bp
  • G to T, chromosome 2 at 128,929,957 bp
  • T to C, chromosome 2 at 154,625,060 bp
  • A to G, chromosome 2 at 156,035,598 bp
  • T to C, chromosome 2 at 165,397,516 bp
  • A to T, chromosome 3 at 28,071,187 bp
  • T to A, chromosome 3 at 98,619,720 bp
  • A to G, chromosome 3 at 159,614,448 bp
  • C to A, chromosome 4 at 121,040,974 bp
  • T to A, chromosome 4 at 147,584,110 bp
  • A to G, chromosome 5 at 12,584,974 bp
  • T to C, chromosome 5 at 31,196,980 bp
  • A to G, chromosome 5 at 112,966,982 bp
  • G to A, chromosome 5 at 145,779,743 bp
  • A to G, chromosome 6 at 48,082,174 bp
  • A to T, chromosome 6 at 71,941,535 bp
  • C to T, chromosome 7 at 4,746,454 bp
  • A to G, chromosome 7 at 73,480,430 bp
  • A to T, chromosome 7 at 79,141,773 bp
  • T to C, chromosome 7 at 108,545,377 bp
  • G to A, chromosome 8 at 61,495,064 bp
  • A to T, chromosome 8 at 104,488,491 bp
  • T to C, chromosome 9 at 37,537,621 bp
  • C to T, chromosome 9 at 55,418,008 bp
  • T to C, chromosome 9 at 59,948,767 bp
  • C to T, chromosome 9 at 71,725,944 bp
  • C to G, chromosome 9 at 90,100,533 bp
  • T to A, chromosome 9 at 108,842,952 bp
  • T to C, chromosome 9 at 110,489,966 bp
  • T to C, chromosome 9 at 119,562,380 bp
  • C to T, chromosome 10 at 127,177,929 bp
  • A to G, chromosome 10 at 127,545,530 bp
  • G to T, chromosome 11 at 4,874,117 bp
  • G to A, chromosome 11 at 29,540,721 bp
  • T to C, chromosome 11 at 51,685,678 bp
  • A to T, chromosome 11 at 59,428,770 bp
  • A to T, chromosome 11 at 74,195,896 bp
  • T to G, chromosome 11 at 84,264,084 bp
  • A to G, chromosome 13 at 3,933,011 bp
  • C to T, chromosome 13 at 21,959,273 bp
  • T to C, chromosome 13 at 33,880,226 bp
  • T to C, chromosome 13 at 67,198,639 bp
  • C to T, chromosome 13 at 104,036,947 bp
  • T to C, chromosome 13 at 112,952,843 bp
  • T to A, chromosome 14 at 48,168,189 bp
  • G to C, chromosome 14 at 49,773,560 bp
  • A to T, chromosome 14 at 63,156,547 bp
  • A to T, chromosome 15 at 4,931,043 bp
  • G to T, chromosome 16 at 91,909,223 bp
  • A to C, chromosome 17 at 5,918,436 bp
  • A to G, chromosome 17 at 14,197,990 bp
  • A to G, chromosome 17 at 24,156,055 bp
  • G to A, chromosome 17 at 34,137,399 bp
  • G to A, chromosome 17 at 52,839,125 bp
  • A to T, chromosome 19 at 5,493,555 bp
  • C to A, chromosome 19 at 23,975,635 bp
  • G to A, chromosome 19 at 27,245,670 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1657 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039693-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.