Strain Name:
C57BL/6J-MtgxR1681Btlr/Mmmh
Stock Number:
039717-MU
Citation ID:
RRID:MMRRC_039717-MU
Other Names:
R1681 (G1), C57BL/6J-MtgxR1681Btlr
Major Collection:

Gene Information

Gene Symbol: Atm [MGI:107202] (Mus musculus (mouse))
Name: ataxia telangiectasia mutated
Synonyms: C030026E19Rik
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: H2afy [MGI:1349392] (Mus musculus (mouse))
Name: H2A histone family, member Y
Synonyms: macroH2A1, mH2a1, MACROH2A1.2, H2AF12M
Chromosome: 13
Alteration at locus: Chemically Induced
Gene Symbol: Scrib [MGI:2145950] (Mus musculus (mouse))
Name: scribbled planar cell polarity
Synonyms: Crc, Scrb1
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Hsd17b12 [MGI:1926967] (Mus musculus (mouse))
Name: hydroxysteroid (17-beta) dehydrogenase 12
Synonyms: 2610510O05Rik, KIK-I, keratonectin, keratoadhesin
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Arhgef18 [MGI:2142567] (Mus musculus (mouse))
Name: rho/rac guanine nucleotide exchange factor (GEF) 18
Synonyms: A430078G23Rik, D030053O22Rik
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Ssr2 [MGI:1913506] (Mus musculus (mouse))
Name: signal sequence receptor, beta
Synonyms: TRAPbeta
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Nfya [MGI:97316] (Mus musculus (mouse))
Name: nuclear transcription factor-Y alpha
Synonyms: Cbf-b, Sez10
Chromosome: 17
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 38,813,276 bp
  • T to A, chromosome 1 at 46,324,712 bp
  • T to A, chromosome 1 at 90,773,502 bp
  • T to C, chromosome 1 at 100,076,107 bp
  • C to T, chromosome 1 at 151,443,236 bp
  • T to A, chromosome 1 at 156,320,898 bp
  • T to C, chromosome 2 at 3,409,274 bp
  • T to C, chromosome 2 at 43,679,825 bp
  • T to C, chromosome 2 at 68,696,536 bp
  • G to A, chromosome 2 at 87,388,620 bp
  • T to C, chromosome 2 at 94,033,561 bp
  • T to C, chromosome 2 at 122,299,162 bp
  • T to C, chromosome 2 at 157,030,530 bp
  • T to C, chromosome 2 at 157,101,732 bp
  • A to G, chromosome 3 at 15,296,675 bp
  • T to C, chromosome 3 at 88,581,042 bp
  • T to C, chromosome 3 at 88,638,358 bp
  • T to A, chromosome 3 at 99,351,824 bp
  • C to T, chromosome 3 at 107,474,386 bp
  • T to C, chromosome 3 at 116,603,473 bp
  • T to A, chromosome 4 at 24,898,559 bp
  • C to T, chromosome 4 at 40,697,274 bp
  • C to T, chromosome 4 at 66,841,105 bp
  • T to C, chromosome 4 at 84,947,635 bp
  • G to A, chromosome 4 at 105,208,805 bp
  • T to A, chromosome 4 at 108,848,858 bp
  • G to C, chromosome 4 at 129,578,276 bp
  • T to A, chromosome 4 at 134,148,728 bp
  • T to A, chromosome 4 at 137,402,684 bp
  • T to A, chromosome 4 at 149,195,501 bp
  • A to G, chromosome 5 at 35,569,977 bp
  • A to T, chromosome 5 at 74,685,410 bp
  • A to T, chromosome 5 at 98,029,580 bp
  • A to T, chromosome 5 at 114,870,818 bp
  • A to T, chromosome 5 at 120,769,908 bp
  • T to A, chromosome 5 at 123,356,023 bp
  • T to C, chromosome 5 at 142,773,817 bp
  • T to A, chromosome 6 at 48,656,411 bp
  • A to T, chromosome 6 at 102,170,668 bp
  • G to T, chromosome 6 at 113,372,456 bp
  • G to A, chromosome 6 at 136,816,236 bp
  • A to G, chromosome 7 at 10,580,252 bp
  • T to A, chromosome 7 at 25,314,734 bp
  • C to A, chromosome 7 at 26,763,340 bp
  • T to C, chromosome 7 at 29,777,400 bp
  • A to C, chromosome 7 at 43,408,941 bp
  • T to A, chromosome 7 at 44,461,177 bp
  • T to G, chromosome 7 at 80,099,158 bp
  • G to A, chromosome 8 at 3,439,645 bp
  • A to C, chromosome 8 at 64,085,551 bp
  • A to G, chromosome 8 at 71,386,052 bp
  • G to T, chromosome 8 at 71,407,618 bp
  • A to C, chromosome 8 at 72,256,122 bp
  • A to T, chromosome 8 at 90,973,135 bp
  • C to A, chromosome 8 at 93,275,414 bp
  • T to C, chromosome 9 at 15,007,783 bp
  • T to A, chromosome 9 at 38,524,117 bp
  • G to A, chromosome 9 at 38,606,948 bp
  • A to G, chromosome 9 at 38,665,320 bp
  • T to C, chromosome 9 at 38,848,513 bp
  • A to C, chromosome 9 at 48,489,721 bp
  • A to C, chromosome 9 at 53,522,155 bp
  • A to G, chromosome 9 at 65,128,795 bp
  • C to A, chromosome 9 at 87,227,431 bp
  • C to T, chromosome 9 at 119,804,412 bp
  • T to C, chromosome 10 at 7,705,914 bp
  • A to G, chromosome 10 at 34,127,900 bp
  • A to T, chromosome 10 at 79,880,541 bp
  • T to G, chromosome 11 at 51,695,189 bp
  • T to A, chromosome 11 at 59,103,325 bp
  • T to A, chromosome 11 at 71,142,358 bp
  • T to A, chromosome 11 at 76,272,464 bp
  • C to T, chromosome 11 at 78,213,256 bp
  • T to A, chromosome 11 at 84,226,185 bp
  • T to C, chromosome 11 at 101,325,192 bp
  • G to A, chromosome 11 at 116,138,429 bp
  • T to C, chromosome 11 at 118,505,669 bp
  • T to C, chromosome 12 at 55,762,603 bp
  • T to C, chromosome 12 at 65,105,656 bp
  • T to A, chromosome 12 at 83,724,620 bp
  • A to G, chromosome 13 at 56,096,242 bp
  • G to T, chromosome 13 at 60,718,464 bp
  • T to C, chromosome 13 at 100,161,854 bp
  • C to T, chromosome 13 at 100,161,860 bp
  • T to A, chromosome 14 at 50,893,077 bp
  • G to A, chromosome 15 at 9,687,983 bp
  • T to C, chromosome 15 at 44,432,478 bp
  • T to C, chromosome 15 at 76,064,567 bp
  • A to G, chromosome 15 at 76,331,453 bp
  • A to T, chromosome 15 at 101,802,047 bp
  • T to C, chromosome 15 at 102,955,156 bp
  • C to A, chromosome 16 at 17,559,753 bp
  • A to G, chromosome 16 at 63,595,728 bp
  • T to A, chromosome 17 at 29,140,124 bp
  • A to G, chromosome 17 at 29,575,177 bp
  • A to T, chromosome 17 at 33,951,213 bp
  • A to T, chromosome 17 at 46,636,770 bp
  • G to A, chromosome 17 at 48,392,956 bp
  • T to C, chromosome 18 at 37,473,813 bp
  • G to T, chromosome 18 at 58,605,138 bp
  • C to A, chromosome 19 at 23,936,561 bp
  • T to C, chromosome 19 at 25,410,304 bp
  • C to A, chromosome 19 at 41,308,529 bp
  • C to A, chromosome X at 86,046,650 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1681 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039717-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.