Strain Name:
C57BL/6J-MtgxR1702Btlr/Mmmh
Stock Number:
039735-MU
Citation ID:
RRID:MMRRC_039735-MU
Other Names:
R1702 (G1), C57BL/6J-MtgxR1702Btlr
Major Collection:

Gene Information

Gene Symbol: Eno1 [MGI:95393] (Mus musculus (mouse))
Name: enolase 1, alpha non-neuron
Synonyms: c-Myc promoter binding protein, Eno-1, MBP-1, 2-phospho-D-glycerate hydrolase, alpha-enolase
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Vcp [MGI:99919] (Mus musculus (mouse))
Name: valosin containing protein
Synonyms: p97, CDC48, AAA ATPase p97, p97/VCP
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Ank2 [MGI:88025] (Mus musculus (mouse))
Name: ankyrin 2, brain
Synonyms: ankyrin B, Ank-2, Ankyrin-B, Ankyrin-2, Gm4392
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Nes [MGI:101784] (Mus musculus (mouse))
Name: nestin
Synonyms: ESTM46, Marc2, Ifaprc2, RC2
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Crhr2 [MGI:894312] (Mus musculus (mouse))
Name: corticotropin releasing hormone receptor 2
Synonyms: CRF-R2, Crfr2, CRF 2 receptor, CRFR2beta, CRH-R2, CRFR2alpha
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Egf [MGI:95290] (Mus musculus (mouse))
Name: epidermal growth factor
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Dst [MGI:104627] (Mus musculus (mouse))
Name: dystonin
Synonyms: ah, 2310001O04Rik, bullous pemphigoid antigen 1, A830042E19Rik, Bpag, Macf2, athetoid, Bpag1, nmf339, nmf203, BPAG1, bullous pemphigoid antigen 1, BPAG1-n
Chromosome: 1
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 34,167,340 bp
  • C to T, chromosome 1 at 43,990,548 bp
  • A to T, chromosome 1 at 84,745,063 bp
  • A to G, chromosome 1 at 130,938,985 bp
  • A to T, chromosome 1 at 134,040,762 bp
  • A to G, chromosome 2 at 25,092,584 bp
  • T to A, chromosome 2 at 32,299,275 bp
  • A to T, chromosome 2 at 35,171,836 bp
  • G to T, chromosome 2 at 39,000,174 bp
  • T to C, chromosome 2 at 58,771,550 bp
  • A to T, chromosome 2 at 62,386,429 bp
  • C to T, chromosome 2 at 66,318,223 bp
  • A to T, chromosome 2 at 90,683,958 bp
  • T to A, chromosome 2 at 118,113,442 bp
  • T to C, chromosome 2 at 120,979,227 bp
  • A to T, chromosome 2 at 150,409,180 bp
  • A to T, chromosome 3 at 53,505,431 bp
  • A to G, chromosome 3 at 87,975,979 bp
  • A to T, chromosome 3 at 126,955,899 bp
  • C to A, chromosome 3 at 129,690,811 bp
  • C to A, chromosome 4 at 20,158,339 bp
  • T to C, chromosome 4 at 42,990,840 bp
  • A to T, chromosome 4 at 123,043,825 bp
  • A to G, chromosome 4 at 139,375,201 bp
  • A to G, chromosome 4 at 146,196,927 bp
  • A to G, chromosome 4 at 150,239,575 bp
  • A to G, chromosome 5 at 8,512,318 bp
  • A to T, chromosome 5 at 24,415,456 bp
  • G to T, chromosome 5 at 84,106,049 bp
  • T to A, chromosome 5 at 93,156,924 bp
  • T to C, chromosome 5 at 108,606,376 bp
  • T to G, chromosome 5 at 113,613,842 bp
  • T to C, chromosome 5 at 137,290,989 bp
  • T to C, chromosome 6 at 40,464,201 bp
  • A to G, chromosome 6 at 55,092,535 bp
  • T to C, chromosome 6 at 116,229,306 bp
  • A to G, chromosome 7 at 4,773,734 bp
  • G to A, chromosome 7 at 23,952,969 bp
  • A to G, chromosome 7 at 28,174,979 bp
  • T to A, chromosome 7 at 42,616,000 bp
  • T to A, chromosome 7 at 42,820,231 bp
  • T to A, chromosome 7 at 51,953,341 bp
  • T to C, chromosome 7 at 56,985,100 bp
  • T to A, chromosome 7 at 85,559,628 bp
  • T to A, chromosome 7 at 118,672,239 bp
  • T to C, chromosome 7 at 118,735,257 bp
  • T to A, chromosome 7 at 119,573,564 bp
  • T to C, chromosome 7 at 120,382,702 bp
  • A to G, chromosome 7 at 127,305,025 bp
  • A to T, chromosome 7 at 140,372,742 bp
  • C to G, chromosome 7 at 141,314,954 bp
  • G to T, chromosome 7 at 141,650,487 bp
  • T to G, chromosome 8 at 106,092,160 bp
  • T to A, chromosome 8 at 126,428,218 bp
  • A to G, chromosome 9 at 38,828,543 bp
  • A to C, chromosome 9 at 44,275,073 bp
  • T to C, chromosome 10 at 27,190,529 bp
  • A to C, chromosome 10 at 121,090,101 bp
  • T to C, chromosome 10 at 127,310,079 bp
  • T to A, chromosome 11 at 66,085,195 bp
  • T to A, chromosome 11 at 78,289,028 bp
  • T to A, chromosome 11 at 94,668,745 bp
  • T to C, chromosome 11 at 102,406,626 bp
  • C to T, chromosome 11 at 104,691,006 bp
  • A to G, chromosome 11 at 116,948,659 bp
  • A to T, chromosome 11 at 121,906,116 bp
  • T to A, chromosome 12 at 24,509,126 bp
  • A to G, chromosome 12 at 65,161,744 bp
  • A to G, chromosome 13 at 76,122,743 bp
  • A to G, chromosome 13 at 114,847,933 bp
  • G to T, chromosome 14 at 20,686,573 bp
  • A to T, chromosome 14 at 34,397,914 bp
  • T to A, chromosome 14 at 54,856,995 bp
  • C to T, chromosome 14 at 55,023,402 bp
  • T to A, chromosome 14 at 70,079,767 bp
  • T to C, chromosome 15 at 88,780,853 bp
  • A to G, chromosome 15 at 89,161,984 bp
  • G to T, chromosome 15 at 89,499,896 bp
  • T to A, chromosome 15 at 102,148,182 bp
  • G to T, chromosome 16 at 19,676,072 bp
  • C to T, chromosome 16 at 20,631,246 bp
  • T to A, chromosome 16 at 34,921,944 bp
  • C to A, chromosome 16 at 44,009,548 bp
  • C to T, chromosome 16 at 44,219,632 bp
  • C to T, chromosome 16 at 56,694,493 bp
  • C to A, chromosome 16 at 59,329,141 bp
  • A to G, chromosome 16 at 93,747,621 bp
  • A to G, chromosome 16 at 97,558,683 bp
  • T to A, chromosome 17 at 25,244,805 bp
  • A to T, chromosome 17 at 78,856,634 bp
  • T to A, chromosome 18 at 67,366,421 bp
  • C to A, chromosome 19 at 12,099,166 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1702 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039735-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.