MMRRC:039897-MU

T to C, chromosome 1 at 30,830,623 bp
A to T, chromosome 1 at 53,456,532 bp
T to C, chromosome 1 at 75,498,233 bp
T to C, chromosome 1 at 119,953,659 bp
A to G, chromosome 1 at 161,970,947 bp
T to C, chromosome 1 at 162,803,618 bp
A to T, chromosome 2 at 120,513,615 bp
T to C, chromosome 3 at 35,839,147 bp
A to T, chromosome 3 at 49,754,705 bp
A to T, chromosome 3 at 83,905,076 bp
A to C, chromosome 3 at 84,595,087 bp
A to T, chromosome 3 at 123,026,116 bp
T to A, chromosome 3 at 133,133,740 bp
G to A, chromosome 4 at 106,613,256 bp
T to C, chromosome 4 at 106,653,716 bp
A to G, chromosome 4 at 118,456,829 bp
A to G, chromosome 4 at 141,308,979 bp
G to A, chromosome 4 at 154,998,508 bp
T to A, chromosome 5 at 4,197,364 bp
C to T, chromosome 5 at 20,951,698 bp
C to T, chromosome 5 at 21,560,652 bp
T to A, chromosome 5 at 21,815,727 bp
T to A, chromosome 5 at 34,794,112 bp
A to T, chromosome 5 at 77,054,584 bp
A to G, chromosome 5 at 104,776,454 bp
G to A, chromosome 5 at 142,886,223 bp
A to G, chromosome 5 at 150,326,132 bp
T to A, chromosome 6 at 48,414,049 bp
G to T, chromosome 6 at 115,978,084 bp
A to G, chromosome 6 at 118,540,449 bp
A to T, chromosome 6 at 121,042,064 bp
A to T, chromosome 7 at 4,629,699 bp
G to A, chromosome 7 at 18,610,450 bp
A to G, chromosome 7 at 100,407,025 bp
G to A, chromosome 7 at 106,972,182 bp
A to G, chromosome 7 at 112,149,055 bp
A to T, chromosome 7 at 116,417,971 bp
A to T, chromosome 7 at 120,247,967 bp
T to C, chromosome 7 at 127,866,482 bp
G to A, chromosome 8 at 13,167,257 bp
T to C, chromosome 8 at 15,929,101 bp
C to A, chromosome 8 at 43,569,851 bp
G to T, chromosome 8 at 53,599,419 bp
A to G, chromosome 8 at 85,352,865 bp
A to T, chromosome 9 at 22,238,731 bp
T to C, chromosome 9 at 32,423,913 bp
A to G, chromosome 9 at 44,401,511 bp
A to G, chromosome 9 at 53,935,867 bp
T to C, chromosome 9 at 108,835,838 bp
A to G, chromosome 10 at 62,594,068 bp
A to G, chromosome 10 at 83,256,085 bp
T to C, chromosome 10 at 107,899,590 bp
T to C, chromosome 10 at 128,574,466 bp
A to G, chromosome 11 at 8,844,670 bp
A to G, chromosome 11 at 21,300,251 bp
A to G, chromosome 11 at 53,465,675 bp
T to A, chromosome 11 at 58,923,760 bp
C to T, chromosome 11 at 60,507,528 bp
A to G, chromosome 11 at 67,953,630 bp
A to T, chromosome 11 at 87,388,643 bp
A to G, chromosome 11 at 100,372,294 bp
CAGCTCAACACGACGGTA to CAGCTCAACACGACGGTAAGCTCAACACGACGGTA, chromosome 11 at 101,586,378 bp
T to C, chromosome 12 at 36,020,551 bp
T to A, chromosome 12 at 100,878,790 bp
C to A, chromosome 12 at 104,151,886 bp
T to C, chromosome 13 at 27,351,054 bp
T to C, chromosome 14 at 49,682,358 bp
C to T, chromosome 15 at 9,584,108 bp
C to T, chromosome 15 at 9,597,401 bp
T to C, chromosome 15 at 36,546,768 bp
T to C, chromosome 15 at 66,931,091 bp
T to C, chromosome 15 at 76,242,851 bp
C to A, chromosome 15 at 77,513,566 bp
G to T, chromosome 15 at 94,331,396 bp
A to G, chromosome 16 at 56,574,499 bp
G to T, chromosome 17 at 6,028,550 bp
G to A, chromosome 17 at 26,917,290 bp
T to C, chromosome 17 at 29,852,607 bp
T to C, chromosome 17 at 30,959,119 bp
T to C, chromosome 17 at 35,060,845 bp
A to G, chromosome 17 at 37,805,105 bp
T to A, chromosome 18 at 16,624,877 bp
G to C, chromosome 18 at 84,949,262 bp
G to A, chromosome 19 at 50,288,074 bp
G to T, chromosome 19 at 58,763,389 bp
T to A, chromosome X at 56,524,767 bp

Genotype Determination

Genotyping Protocol(s)
Center protocol and contact for technical support will be shipped with mice.

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.

Strain Development

C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.

Suggested Control Mice

Wild-type littermates or C57BL/6J mice

Strain Origin

Donor

Bruce Beutler, M.D., University of Texas Southwestern Medical Center.

Primary Reference

MUTAGENETIX record for R1875 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information Expand All

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .

Appearance

Coat Color

Black

Breeding

MMRRC Breeding System

Random intra-strain mating

Breeding Scheme(s)

When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.

Overall Breeding Performance

Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size

Undetermined

Recommended wean age

3 weeks

Order Request Information Expand All

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)

MMRRC Item #	Description	Distribution Fee / Unit (US $) Shipping & Handling not included	Units	Notes
039897-MU-RESUS	Litter recovered from cryo-archive	$5,475.00 / Non-Profit	Litter	Recovered litter⁴; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

¹ The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

² An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

³ An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

⁴ The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.

Strain Detail Sheet

Gene Information Expand All

Colony and Husbandry Information Expand All

Order Request Information Expand All