Strain Name:
C57BL/6J-MtgxR2116Btlr/Mmmh
Stock Number:
040120-MU
Citation ID:
RRID:MMRRC_040120-MU
Other Names:
R2116 (G1), C57BL/6J-MtgxR2116Btlr
Major Collection:

Gene Information

Gene Symbol: Adamts20 [MGI:2660628] (Mus musculus (mouse))
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 20
Synonyms: bt, ADAMTS-20
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Suz12 [MGI:1261758] (Mus musculus (mouse))
Name: SUZ12 polycomb repressive complex 2 subunit
Synonyms: D11Ertd530e, 2610028O16Rik
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Elp2 [MGI:1889642] (Mus musculus (mouse))
Name: elongator acetyltransferase complex subunit 2
Synonyms: Statip1, Stat3-interacting protein, StIP1
Chromosome: 18
Alteration at locus: Chemically Induced
Gene Symbol: Ptx3 [MGI:104641] (Mus musculus (mouse))
Name: pentraxin related gene
Synonyms: TSG-14, pentraxin 3
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Plg [MGI:97620] (Mus musculus (mouse))
Name: plasminogen
Synonyms: Pg
Chromosome: 17
Alteration at locus: Chemically Induced
Gene Symbol: Lyst [MGI:107448] (Mus musculus (mouse))
Name: lysosomal trafficking regulator
Synonyms: D13Sfk13
Chromosome: 13
Alteration at locus: Chemically Induced
Gene Symbol: Cacng6 [MGI:1859168] (Mus musculus (mouse))
Name: calcium channel, voltage-dependent, gamma subunit 6
Synonyms: 2310033H20Rik
Chromosome: 7
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 52,660,975 bp
  • A to T, chromosome 1 at 120,031,582 bp
  • T to A, chromosome 1 at 153,817,549 bp
  • A to T, chromosome 1 at 156,267,386 bp
  • C to A, chromosome 1 at 158,969,846 bp
  • T to A, chromosome 1 at 161,109,805 bp
  • T to A, chromosome 1 at 171,218,594 bp
  • T to A, chromosome 1 at 175,091,073 bp
  • T to C, chromosome 1 at 182,975,629 bp
  • A to G, chromosome 2 at 18,162,569 bp
  • T to A, chromosome 2 at 31,016,305 bp
  • C to T, chromosome 2 at 31,369,074 bp
  • T to C, chromosome 2 at 32,367,465 bp
  • C to A, chromosome 2 at 36,237,696 bp
  • T to C, chromosome 2 at 51,948,707 bp
  • A to T, chromosome 2 at 86,162,729 bp
  • A to G, chromosome 2 at 121,954,552 bp
  • A to G, chromosome 2 at 126,358,756 bp
  • T to A, chromosome 2 at 162,960,070 bp
  • T to A, chromosome 2 at 165,491,495 bp
  • A to C, chromosome 2 at 167,027,764 bp
  • C to T, chromosome 2 at 181,687,600 bp
  • T to A, chromosome 3 at 26,133,265 bp
  • T to A, chromosome 3 at 29,965,458 bp
  • G to T, chromosome 3 at 66,057,189 bp
  • T to A, chromosome 3 at 66,224,766 bp
  • C to T, chromosome 3 at 88,983,264 bp
  • T to C, chromosome 3 at 89,219,392 bp
  • T to C, chromosome 3 at 129,810,687 bp
  • T to C, chromosome 3 at 134,861,229 bp
  • G to A, chromosome 4 at 16,008,648 bp
  • A to T, chromosome 4 at 34,566,012 bp
  • A to G, chromosome 4 at 94,627,259 bp
  • A to T, chromosome 4 at 101,179,675 bp
  • G to A, chromosome 4 at 101,967,651 bp
  • G to T, chromosome 4 at 111,415,239 bp
  • A to G, chromosome 4 at 148,158,522 bp
  • C to A, chromosome 5 at 22,039,680 bp
  • A to T, chromosome 5 at 106,455,683 bp
  • A to T, chromosome 5 at 110,187,395 bp
  • A to T, chromosome 5 at 115,598,825 bp
  • A to G, chromosome 5 at 125,421,102 bp
  • A to G, chromosome 5 at 125,622,551 bp
  • T to A, chromosome 5 at 136,130,478 bp
  • T to A, chromosome 6 at 21,216,432 bp
  • A to T, chromosome 6 at 96,164,860 bp
  • A to G, chromosome 6 at 129,121,040 bp
  • C to T, chromosome 7 at 3,430,504 bp
  • A to T, chromosome 7 at 18,794,255 bp
  • T to A, chromosome 7 at 43,297,946 bp
  • C to T, chromosome 7 at 46,996,185 bp
  • G to T, chromosome 7 at 55,985,525 bp
  • G to A, chromosome 7 at 67,733,595 bp
  • T to C, chromosome 7 at 86,616,078 bp
  • T to C, chromosome 7 at 103,683,312 bp
  • T to A, chromosome 7 at 110,051,118 bp
  • C to T, chromosome 7 at 121,648,696 bp
  • A to G, chromosome 7 at 122,101,764 bp
  • T to A, chromosome 7 at 126,385,108 bp
  • T to C, chromosome 7 at 126,702,022 bp
  • T to C, chromosome 7 at 127,810,905 bp
  • T to G, chromosome 7 at 128,198,515 bp
  • G to A, chromosome 7 at 128,252,805 bp
  • A to T, chromosome 8 at 11,550,247 bp
  • A to T, chromosome 8 at 19,023,467 bp
  • G to A, chromosome 8 at 22,706,067 bp
  • A to G, chromosome 8 at 71,407,918 bp
  • T to C, chromosome 8 at 71,557,828 bp
  • A to T, chromosome 8 at 71,932,165 bp
  • A to C, chromosome 8 at 95,726,153 bp
  • A to T, chromosome 8 at 104,215,178 bp
  • T to A, chromosome 8 at 117,030,722 bp
  • T to A, chromosome 9 at 39,737,304 bp
  • T to C, chromosome 9 at 44,299,615 bp
  • A to G, chromosome 9 at 51,101,084 bp
  • C to T, chromosome 9 at 53,500,969 bp
  • A to C, chromosome 9 at 114,626,436 bp
  • A to G, chromosome 10 at 18,124,920 bp
  • A to T, chromosome 10 at 41,963,667 bp
  • A to G, chromosome 10 at 53,475,456 bp
  • C to A, chromosome 10 at 78,063,296 bp
  • A to T, chromosome 10 at 79,194,724 bp
  • A to T, chromosome 10 at 79,865,738 bp
  • C to T, chromosome 10 at 127,576,493 bp
  • T to A, chromosome 11 at 71,114,500 bp
  • T to C, chromosome 11 at 75,487,721 bp
  • TC to T, chromosome 11 at 77,786,002 bp
  • T to C, chromosome 11 at 79,995,676 bp
  • T to A, chromosome 11 at 83,757,455 bp
  • A to C, chromosome 11 at 99,365,117 bp
  • T to C, chromosome 11 at 115,605,960 bp
  • A to G, chromosome 13 at 13,635,701 bp
  • G to T, chromosome 13 at 23,249,662 bp
  • G to A, chromosome 13 at 24,428,897 bp
  • A to C, chromosome 13 at 47,014,185 bp
  • A to T, chromosome 13 at 58,018,991 bp
  • T to C, chromosome 13 at 81,529,013 bp
  • A to G, chromosome 13 at 95,015,029 bp
  • G to T, chromosome 13 at 99,430,644 bp
  • G to A, chromosome 14 at 74,785,531 bp
  • T to A, chromosome 14 at 119,869,287 bp
  • T to G, chromosome 15 at 34,515,832 bp
  • G to T, chromosome 15 at 44,569,482 bp
  • A to G, chromosome 15 at 55,407,764 bp
  • A to G, chromosome 15 at 75,926,142 bp
  • G to T, chromosome 15 at 86,132,918 bp
  • A to G, chromosome 15 at 94,355,362 bp
  • A to T, chromosome 17 at 12,384,477 bp
  • T to C, chromosome 17 at 34,672,227 bp
  • A to T, chromosome 17 at 36,039,057 bp
  • A to C, chromosome 17 at 46,315,568 bp
  • A to T, chromosome 17 at 90,704,277 bp
  • C to T, chromosome 18 at 12,571,897 bp
  • T to A, chromosome 18 at 24,606,850 bp
  • T to A, chromosome 18 at 49,878,817 bp
  • G to A, chromosome 18 at 53,740,136 bp
  • T to C, chromosome 18 at 60,832,785 bp
  • A to T, chromosome 18 at 65,800,649 bp
  • A to G, chromosome 19 at 6,386,408 bp
  • T to C, chromosome 19 at 8,754,904 bp
  • T to C, chromosome 19 at 8,943,516 bp
  • C to A, chromosome 19 at 36,614,424 bp
  • T to A, chromosome 19 at 46,056,576 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2116 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040120-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.