Strain Name:
C57BL/6J-MtgxR2199Btlr/Mmmh
Stock Number:
040201-MU
Citation ID:
RRID:MMRRC_040201-MU
Other Names:
R2199 (G1), C57BL/6J-MtgxR2199Btlr
Major Collection:

Gene Information

Traf4
Name: TNF receptor associated factor 4
Synonyms: CART1, A530032M13Rik, msp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 22032
Homologene: 3173
Dysf
Name: dysferlin
Synonyms: 2310004N10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 26903
HGNC: HGNC:3097
Homologene: 20748
Itch
Name: itchy, E3 ubiquitin protein ligase
Synonyms: 6720481N21Rik, C230047C07Rik, 8030492O04Rik, AIP4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16396
Homologene: 88442
Gli2
Name: GLI-Kruppel family member GLI2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 14633
HGNC: HGNC:4318
Homologene: 12725
Ctdspl2
Name: CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase like 2
Synonyms: D2Ertd485e, SCP4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 329506
Homologene: 32311
Arhgap12
Name: Rho GTPase activating protein 12
Synonyms: 2810011M08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 75415
Homologene: 23089
Ube2o
Name: ubiquitin-conjugating enzyme E2O
Synonyms: B230113M03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217342
Homologene: 11113
Smg7
Name: SMG7 nonsense mediated mRNA decay factor
Synonyms: 9430023P16Rik, Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226517
Homologene: 32235
Nmt1
Name: N-myristoyltransferase 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18107
HGNC: HGNC:7857
Homologene: 69027
Arfgap2
Name: ADP-ribosylation factor GTPase activating protein 2
Synonyms: 2310032E02Rik, Zfp289
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 77038
Homologene: 8735
Xrn2
Name: 5'-3' exoribonuclease 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 24128
Homologene: 6927
Ccm2
Name: cerebral cavernous malformation 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216527
Homologene: 12868
Otud7b
Name: OTU domain containing 7B
Synonyms: 4930463P07Rik, 2900060B22Rik, Za20d1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229603
Homologene: 10624
Dmxl2
Name: Dmx-like 2
Synonyms: E130119P06Rik, 6430411K14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235380
HGNC: HGNC:2938
Homologene: 41022
Abca17
Name: ATP-binding cassette, sub-family A (ABC1), member 17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 381072
Homologene: 86807
Irx4
Name: Iroquois homeobox 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 50916
HGNC: HGNC:6129
Homologene: 9485
Grhl1
Name: grainyhead like transcription factor 1
Synonyms: p70 MGR, p61 MGR, LBP-32, Tcfcp2l2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 195733
VEGA: 12
Homologene: 32219
Il20
Name: interleukin 20
Synonyms: Zcyto10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 58181
HGNC: HGNC:6002
Homologene: 10286
Dnajc1
Name: DnaJ heat shock protein family (Hsp40) member C1
Synonyms: MTJ1, Dnajl1, ERdj1, 4733401K02Rik, D230036H06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 13418
Homologene: 7293
Slc44a3
Name: solute carrier family 44, member 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 213603
Homologene: 64830
Trpv1
Name: transient receptor potential cation channel, subfamily V, member 1
Synonyms: capsaicin receptor, VR-1, OTRPC1, Vr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 193034
Homologene: 12920
Ltbr
Name: lymphotoxin B receptor
Synonyms: TNFRrp, TNF receptor-related protein, LT-beta receptor, LT beta-R, Tnfbr, Ltar, TNF-R-III, TNFCR, TNFR2-RP, LTbetaR, Tnfrsf3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 17000
HGNC: HGNC:6718
Homologene: 1753
Sall3
Name: spalt like transcription factor 3
Synonyms: Msal, Msal-1, Spalt, Salt, B130022O04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 20689
VEGA: 18
Homologene: 18142
Hormad1
Name: HORMA domain containing 1
Synonyms: 4921522K05Rik, Nohma
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 67981
Homologene: 69415
Dmpk
Name: dystrophia myotonica-protein kinase
Synonyms: DM, Dm15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 13400
HGNC: HGNC:2933
Homologene: 3247
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Lrp1
Name: low density lipoprotein receptor-related protein 1
Synonyms: CD91, A2mr, b2b1554Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 16971
HGNC: HGNC:6692
Homologene: 1744
Rnf213
Name: ring finger protein 213
Synonyms: D11Ertd759e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 672511
Homologene: 45439
Dnah3
Name: dynein, axonemal, heavy chain 3
Synonyms: Dnahc3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 381917
HGNC: HGNC:2949
Homologene: 19674
Ints11
Name: integrator complex subunit 11
Synonyms: 2410006F12Rik, Cpsf3l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 71957
Homologene: 9894
Otud7a
Name: OTU domain containing 7A
Synonyms: Cezanne 2 protein, Otud7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 170711
Homologene: 15642
Nlrc5
Name: NLR family, CARD domain containing 5
Synonyms: AI451557
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 434341
Homologene: 88935
Lrrd1
Name: leucine rich repeats and death domain containing 1
Synonyms: 4932412H11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 242838
Homologene: 18247
Synpo2l
Name: synaptopodin 2-like
Synonyms: 1110054M18Rik, Chap
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 68760
Homologene: 23499
Lrriq1
Name: leucine-rich repeats and IQ motif containing 1
Synonyms: LOC380658, 4930503E15Rik, Gm1557
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 74978
Homologene: 46007
Megf8
Name: multiple EGF-like-domains 8
Synonyms: Egfl4, b2b288Clo, b2b1702Clo, m687Ddg
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 269878
HGNC: HGNC:3233
Homologene: 15988
Tmprss11f
Name: transmembrane protease, serine 11f
Synonyms: 4732406D01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 243083
Homologene: 65356
Nsd3
Name: nuclear receptor binding SET domain protein 3
Synonyms: WHISTLE, Whsc1l1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234135
Homologene: 56960
Wdr46
Name: WD repeat domain 46
Synonyms: 2310007I04Rik, Bing4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 57315
Homologene: 3981
Thsd7b
Name: thrombospondin, type I, domain containing 7B
Synonyms: 1700074E13Rik, D130067I03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 210417
Homologene: 18180
Zfp616
Name: zinc finger protein 616
Synonyms: Gm12330
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 327963
Homologene: 88945
Kctd8
Name: potassium channel tetramerisation domain containing 8
Synonyms: A730087N02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 243043
Homologene: 18464
Lrrc39
Name: leucine rich repeat containing 39
Synonyms: 2010005E21Rik, 9430028I06Rik, Myomasp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 109245
Homologene: 13668
Olfr828
Name: olfactory receptor 828
Synonyms: GA_x6K02T2PVTD-12559294-12558356, MOR149-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258598
Homologene: 133690
Klhl31
Name: kelch-like 31
Synonyms: D930047P17Rik, Kbtbd1, 9830147P19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 244923
Homologene: 27819
Gnrhr
Name: gonadotropin releasing hormone receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 14715
HGNC: HGNC:4421
Homologene: 350
Mfsd1
Name: major facilitator superfamily domain containing 1
Synonyms: 1200003O06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 66868
Homologene: 11228
Slc9a3r2
Name: solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 2
Synonyms: Nherf2, Octs2, Tka-1, Sip-1, E3karp, 1200011K07Rik, Sip1, 2010007A20Rik, 0610011L07Rik, Sryip1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 65962
VEGA: 17
Homologene: 56962
Olfr1215
Name: olfactory receptor 1215
Synonyms: GA_x6K02T2Q125-50482823-50481885, MOR233-13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258451
Homologene: 74071
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 118,837,648 bp
  • A to G, chromosome 1 at 130,218,158 bp
  • T to A, chromosome 1 at 130,910,739 bp
  • C to A, chromosome 1 at 152,854,328 bp
  • A to C, chromosome 2 at 18,308,899 bp
  • C to T, chromosome 2 at 76,754,806 bp
  • A to G, chromosome 2 at 89,001,550 bp
  • T to A, chromosome 2 at 91,265,692 bp
  • T to C, chromosome 2 at 121,987,029 bp
  • C to T, chromosome 2 at 147,024,750 bp
  • A to C, chromosome 2 at 155,202,221 bp
  • A to G, chromosome 3 at 67,587,349 bp
  • T to C, chromosome 3 at 95,567,722 bp
  • A to G, chromosome 3 at 96,155,772 bp
  • A to G, chromosome 3 at 116,570,961 bp
  • T to C, chromosome 3 at 121,513,744 bp
  • A to G, chromosome 4 at 155,875,281 bp
  • A to T, chromosome 5 at 3,866,478 bp
  • C to A, chromosome 5 at 69,341,245 bp
  • T to C, chromosome 5 at 86,197,818 bp
  • A to T, chromosome 5 at 86,544,768 bp
  • A to T, chromosome 6 at 84,113,715 bp
  • T to C, chromosome 6 at 125,312,061 bp
  • C to A, chromosome 7 at 19,086,486 bp
  • T to A, chromosome 7 at 25,339,614 bp
  • A to G, chromosome 7 at 63,757,656 bp
  • C to T, chromosome 7 at 119,951,569 bp
  • G to T, chromosome 8 at 25,666,057 bp
  • G to T, chromosome 8 at 94,516,319 bp
  • C to A, chromosome 9 at 18,815,923 bp
  • T to C, chromosome 9 at 54,376,243 bp
  • T to C, chromosome 9 at 77,650,101 bp
  • T to C, chromosome 10 at 74,170,509 bp
  • A to G, chromosome 10 at 103,068,913 bp
  • A to T, chromosome 10 at 127,546,840 bp
  • T to C, chromosome 11 at 6,590,790 bp
  • A to G, chromosome 11 at 73,240,251 bp
  • C to T, chromosome 11 at 74,084,630 bp
  • T to C, chromosome 11 at 78,159,980 bp
  • T to A, chromosome 11 at 103,063,856 bp
  • A to G, chromosome 11 at 116,544,745 bp
  • A to T, chromosome 11 at 119,460,009 bp
  • G to T, chromosome 12 at 24,612,170 bp
  • A to T, chromosome 13 at 73,265,601 bp
  • A to G, chromosome 14 at 20,661,919 bp
  • T to C, chromosome 17 at 24,335,624 bp
  • T to C, chromosome 17 at 24,640,596 bp
  • T to C, chromosome 17 at 33,940,873 bp
  • A to T, chromosome 18 at 6,057,601 bp
  • T to C, chromosome 18 at 80,971,870 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2199 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040201-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.