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Strain Name:
C57BL/6J-MtgxR2199Btlr/Mmmh
Stock Number:
040201-MU
Citation ID:
RRID:MMRRC_040201-MU
Other Names:
R2199 (G1), C57BL/6J-MtgxR2199Btlr
Major Collection:

Strain Information

Traf4
Name: TNF receptor associated factor 4
Synonyms: CART1, A530032M13Rik, msp2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 22032
Homologene: 3173
Itch
Name: itchy, E3 ubiquitin protein ligase
Synonyms: 6720481N21Rik, C230047C07Rik, 8030492O04Rik, AIP4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16396
Homologene: 88442
Gli2
Name: GLI-Kruppel family member GLI2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14633
HGNC: HGNC:4318
Homologene: 12725
Ctdspl2
Name: CTD small phosphatase like 2
Synonyms: D2Ertd485e, SCP4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 329506
Homologene: 32311
Arhgap12
Name: Rho GTPase activating protein 12
Synonyms: 2810011M08Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 75415
Homologene: 23089
Ube2o
Name: ubiquitin-conjugating enzyme E2O
Synonyms: B230113M03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217342
Homologene: 11113
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 118,837,648 bp
  • A to G, chromosome 1 at 130,218,158 bp
  • T to A, chromosome 1 at 130,910,739 bp
  • C to A, chromosome 1 at 152,854,328 bp
  • A to C, chromosome 2 at 18,308,899 bp
  • C to T, chromosome 2 at 76,754,806 bp
  • A to G, chromosome 2 at 89,001,550 bp
  • T to A, chromosome 2 at 91,265,692 bp
  • T to C, chromosome 2 at 121,987,029 bp
  • C to T, chromosome 2 at 147,024,750 bp
  • A to C, chromosome 2 at 155,202,221 bp
  • A to G, chromosome 3 at 67,587,349 bp
  • T to C, chromosome 3 at 95,567,722 bp
  • A to G, chromosome 3 at 96,155,772 bp
  • A to G, chromosome 3 at 116,570,961 bp
  • T to C, chromosome 3 at 121,513,744 bp
  • A to G, chromosome 4 at 155,875,281 bp
  • A to T, chromosome 5 at 3,866,478 bp
  • C to A, chromosome 5 at 69,341,245 bp
  • T to C, chromosome 5 at 86,197,818 bp
  • A to T, chromosome 5 at 86,544,768 bp
  • A to T, chromosome 6 at 84,113,715 bp
  • T to C, chromosome 6 at 125,312,061 bp
  • C to A, chromosome 7 at 19,086,486 bp
  • T to A, chromosome 7 at 25,339,614 bp
  • A to G, chromosome 7 at 63,757,656 bp
  • C to T, chromosome 7 at 119,951,569 bp
  • G to T, chromosome 8 at 25,666,057 bp
  • G to T, chromosome 8 at 94,516,319 bp
  • C to A, chromosome 9 at 18,815,923 bp
  • T to C, chromosome 9 at 54,376,243 bp
  • T to C, chromosome 9 at 77,650,101 bp
  • T to C, chromosome 10 at 74,170,509 bp
  • A to G, chromosome 10 at 103,068,913 bp
  • A to T, chromosome 10 at 127,546,840 bp
  • T to C, chromosome 11 at 6,590,790 bp
  • A to G, chromosome 11 at 73,240,251 bp
  • C to T, chromosome 11 at 74,084,630 bp
  • T to C, chromosome 11 at 78,159,980 bp
  • T to A, chromosome 11 at 103,063,856 bp
  • A to G, chromosome 11 at 116,544,745 bp
  • A to T, chromosome 11 at 119,460,009 bp
  • G to T, chromosome 12 at 24,612,170 bp
  • A to T, chromosome 13 at 73,265,601 bp
  • A to G, chromosome 14 at 20,661,919 bp
  • T to C, chromosome 17 at 24,335,624 bp
  • T to C, chromosome 17 at 24,640,596 bp
  • T to C, chromosome 17 at 33,940,873 bp
  • A to T, chromosome 18 at 6,057,601 bp
  • T to C, chromosome 18 at 80,971,870 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2199 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040201-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.