Strain Name:
C57BL/6J-MtgxR2223Btlr/Mmmh
Stock Number:
040224-MU
Citation ID:
RRID:MMRRC_040224-MU
Other Names:
R2223 (G1), C57BL/6J-MtgxR2223Btlr
Major Collection:

Gene Information

Ntrk3
Name: neurotrophic tyrosine kinase, receptor, type 3
Synonyms: TrkC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18213
HGNC: HGNC:8033
Homologene: 49183
Cradd
Name: CASP2 and RIPK1 domain containing adaptor with death domain
Synonyms: RAIDD
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 12905
VEGA: 10
HGNC: HGNC:2340
Homologene: 2821
Trip10
Name: thyroid hormone receptor interactor 10
Synonyms: Cip4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 106628
VEGA: 17
Homologene: 99728
Prdm2
Name: PR domain containing 2, with ZNF domain
Synonyms: Riz, Riz1, LOC381568, E330024L24Rik, 4833427P12Rik, KMT8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 110593
HGNC: HGNC:9347
Homologene: 40822
Ttc39b
Name: tetratricopeptide repeat domain 39B
Synonyms: 9130422G05Rik, 1810054D07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 69863
Homologene: 25228
Luc7l2
Name: LUC7-like 2 (S. cerevisiae)
Synonyms: CGI-74, CGI-59, 4930471C18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 192196
Homologene: 56737
Setx
Name: senataxin
Synonyms: A930037J23Rik, Als4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 269254
HGNC: HGNC:445
Homologene: 41003
Cry1
Name: cryptochrome 1 (photolyase-like)
Synonyms: Phll1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 12952
VEGA: 10
HGNC: HGNC:2384
Homologene: 7042
Zfp516
Name: zinc finger protein 516
Synonyms: C330029B10Rik, Zfp26l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 329003
VEGA: 18
Homologene: 37122
Emsy
Name: EMSY, BRCA2-interacting transcriptional repressor
Synonyms: 2210018M11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233545
Homologene: 32465
Acvr1b
Name: activin A receptor, type 1B
Synonyms: ActR-IB, Acvrlk4, SKR2, ActRIB, Alk4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 11479
HGNC: HGNC:172
Homologene: 20906
Afdn
Name: afadin, adherens junction formation factor
Synonyms: AF6, Afadin, S-afadin, I-afadin, 5033403D15Rik, Mllt4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 17356
HGNC: HGNC:7137
Homologene: 21202
Stk39
Name: serine/threonine kinase 39
Synonyms: DCHT, SPAK, RF005, Rnl5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 53416
Homologene: 22739
Uvssa
Name: UV stimulated scaffold protein A
Synonyms: D330017J19Rik, 4933407H18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 71101
Homologene: 13807
Snap91
Name: synaptosomal-associated protein 91
Synonyms: F1-20, 91kDa, AP180
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 20616
Homologene: 8429
Adamtsl1
Name: ADAMTS-like 1
Synonyms: punctin-1, 6720426B09Rik, 5930437A14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 77739
Homologene: 64642
Glul
Name: glutamate-ammonia ligase (glutamine synthetase)
Synonyms: Glns, GS
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 14645
HGNC: HGNC:4341
Homologene: 37567
Kctd10
Name: potassium channel tetramerisation domain containing 10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 330171
Homologene: 32652
Pkd1l1
Name: polycystic kidney disease 1 like 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 171395
Homologene: 51376
Trpa1
Name: transient receptor potential cation channel, subfamily A, member 1
Synonyms: ANKTM1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 277328
HGNC: HGNC:497
Homologene: 7189
Sp8
Name: trans-acting transcription factor 8
Synonyms: mBtd, D930049B17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 320145
VEGA: 12
Homologene: 18548
Atp8b1
Name: ATPase, class I, type 8B, member 1
Synonyms: FIC1, Ic
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 54670
VEGA: 18
HGNC: HGNC:3706
Homologene: 21151
Aff2
Name: AF4/FMR2 family, member 2
Synonyms: Ox19, Oxh, Fmr2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 14266
HGNC: HGNC:3776
Homologene: 1532
Col9a2
Name: collagen, type IX, alpha 2
Synonyms: Col9a-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 12840
HGNC: HGNC:2218
Homologene: 37535
Map3k5
Name: mitogen-activated protein kinase kinase kinase 5
Synonyms: ASK1, Mekk5, 7420452D20Rik, ASK
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 26408
HGNC: HGNC:6857
Homologene: 38114
Nlrp1b
Name: NLR family, pyrin domain containing 1B
Synonyms: Nalp1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 637515
Homologene: 19080
Mx1
Name: MX dynamin-like GTPase 1
Synonyms: myxovirus (influenza) resistance 1 polypeptide, Mx-1, Mx
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 17857
Magi2
Name: membrane associated guanylate kinase, WW and PDZ domain containing 2
Synonyms: S-SCAM, Acvrinp1, Magi-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 50791
Homologene: 8189
Cdcp3
Name: CUB domain containing protein 3
Synonyms: 5430419D17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 71395
Homologene: 138430
B4galt3
Name: UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 3
Synonyms: beta4GalT-III, ESTM26, 9530061M23Rik, R74981
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 57370
HGNC: HGNC:926
Homologene: 48241
Olfr800
Name: olfactory receptor 800
Synonyms: GA_x6K02T2PULF-11338429-11339364, MOR114-7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 258541
Homologene: 27203
Cryz
Name: crystallin, zeta
Synonyms: quinone reductase, Sez9, SEZ9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 12972
HGNC: HGNC:2419
Homologene: 133907
Ptprn
Name: protein tyrosine phosphatase, receptor type, N
Synonyms: IA-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 19275
HGNC: HGNC:9676
Homologene: 48142
Fmo2
Name: flavin containing monooxygenase 2
Synonyms: 2310008D08Rik, 2310042I22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 55990
HGNC: HGNC:3770
Homologene: 86882
Abhd18
Name: abhydrolase domain containing 18
Synonyms: 3110057O12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 269423
Homologene: 105457
Cyp2d11
Name: cytochrome P450, family 2, subfamily d, polypeptide 11
Synonyms: Cyp2d, P450-2D
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 545123
VEGA: 15
Homologene: 86099
Mroh1
Name: maestro heat-like repeat family member 1
Synonyms: D330001F17Rik, Heatr7a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223658
Homologene: 44882
Cntnap5b
Name: contactin associated protein-like 5B
Synonyms: Caspr5-2, C230078M14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 241175
Homologene: 104295
Megf11
Name: multiple EGF-like-domains 11
Synonyms: 2410080H04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 214058
Homologene: 13031
Vmn2r112
Name: vomeronasal 2, receptor 112
Synonyms: EG628185
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 628185
Homologene: 86604
Lrrc38
Name: leucine rich repeat containing 38
Synonyms: A230053A07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242735
Homologene: 77949
Vmn1r25
Name: vomeronasal 1 receptor 25
Synonyms: V1rc8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 113865
Homologene: 137652
Pde8b
Name: phosphodiesterase 8B
Synonyms: B230331L10Rik, C030047E14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218461
HGNC: HGNC:8794
Homologene: 2758
Cfd
Name: complement factor D (adipsin)
Synonyms: D component (adipsin) of complement, DF, factor D, Adn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 11537
VEGA: 10
HGNC: HGNC:2771
Homologene: 20453
Adamts5
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2)
Synonyms: ADAM-TS5, 9530092O11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 23794
VEGA: 16
HGNC: HGNC:221
Homologene: 5109
Exoc3l4
Name: exocyst complex component 3-like 4
Synonyms: 1600013K19Rik, 1200009I06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 74190
VEGA: 12
Homologene: 41760
Fam184a
Name: family with sequence similarity 184, member A
Synonyms: 3110012E06Rik, 4930438C08Rik, 4930589M24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 75906
Homologene: 11600
Ube2w
Name: ubiquitin-conjugating enzyme E2W (putative)
Synonyms: 6130401J04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 66799
Homologene: 10119
Aph1b
Name: aph1 homolog B, gamma secretase subunit
Synonyms: 2310057K14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 208117
Homologene: 12844
Vmn2r71
Name: vomeronasal 2, receptor 71
Synonyms: EG233445
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233445
Homologene: 115466
Mgat4d
Name: MGAT4 family, member C
Synonyms: 4933434I20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 67555
Homologene: 128538
Aspg
Name: asparaginase
Synonyms: A530050D06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 104816
VEGA: 12
Homologene: 113390
Gtpbp2
Name: GTP binding protein 2
Synonyms: nmf205
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 56055
HGNC: HGNC:4670
Homologene: 10420
Gm5174
Name: predicted gene 5174
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 382395
VEGA: 10
Homologene: 141143
Sva
Name: seminal vesicle antigen
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 20939
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 14,903,256 bp
  • A to G, chromosome 1 at 16,597,959 bp
  • A to T, chromosome 1 at 75,257,937 bp
  • A to G, chromosome 1 at 100,213,687 bp
  • T to A, chromosome 1 at 153,906,497 bp
  • C to T, chromosome 1 at 162,898,244 bp
  • C to A, chromosome 1 at 171,274,043 bp
  • T to G, chromosome 2 at 29,148,537 bp
  • GTGGCT to GT, chromosome 2 at 29,154,061 bp
  • C to T, chromosome 2 at 68,314,579 bp
  • A to G, chromosome 3 at 40,934,861 bp
  • A to T, chromosome 3 at 154,618,554 bp
  • T to C, chromosome 4 at 83,232,762 bp
  • A to C, chromosome 4 at 86,388,525 bp
  • C to G, chromosome 4 at 121,054,258 bp
  • T to C, chromosome 4 at 143,134,899 bp
  • T to A, chromosome 4 at 143,369,849 bp
  • T to A, chromosome 5 at 20,465,672 bp
  • C to T, chromosome 5 at 33,392,063 bp
  • G to T, chromosome 5 at 114,367,349 bp
  • T to C, chromosome 6 at 38,565,724 bp
  • T to A, chromosome 6 at 42,038,423 bp
  • A to C, chromosome 6 at 57,979,238 bp
  • T to C, chromosome 7 at 78,198,852 bp
  • T to A, chromosome 7 at 85,624,093 bp
  • T to C, chromosome 7 at 98,590,775 bp
  • A to T, chromosome 7 at 131,247,457 bp
  • G to A, chromosome 8 at 83,355,672 bp
  • G to A, chromosome 9 at 64,660,431 bp
  • A to T, chromosome 9 at 66,784,639 bp
  • T to C, chromosome 9 at 86,792,527 bp
  • T to C, chromosome 10 at 20,067,920 bp
  • G to A, chromosome 10 at 53,655,079 bp
  • A to T, chromosome 10 at 79,892,205 bp
  • A to G, chromosome 10 at 85,143,753 bp
  • T to A, chromosome 10 at 86,656,508 bp
  • A to C, chromosome 10 at 95,175,873 bp
  • T to A, chromosome 10 at 129,659,809 bp
  • T to C, chromosome 11 at 8,889,063 bp
  • T to C, chromosome 11 at 8,950,422 bp
  • A to G, chromosome 11 at 71,155,989 bp
  • C to G, chromosome 12 at 111,426,152 bp
  • G to A, chromosome 12 at 112,114,434 bp
  • G to A, chromosome 12 at 118,849,738 bp
  • T to C, chromosome 13 at 95,043,447 bp
  • T to C, chromosome 15 at 76,408,045 bp
  • T to A, chromosome 15 at 82,390,131 bp
  • C to T, chromosome 15 at 101,203,043 bp
  • A to C, chromosome 16 at 85,899,306 bp
  • T to C, chromosome 16 at 97,455,232 bp
  • T to C, chromosome 17 at 13,883,737 bp
  • T to G, chromosome 17 at 22,601,233 bp
  • A to G, chromosome 17 at 46,167,227 bp
  • A to G, chromosome 17 at 57,263,039 bp
  • T to A, chromosome 18 at 64,564,357 bp
  • C to A, chromosome 18 at 82,955,770 bp
  • T to G, chromosome X at 69,694,001 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2223 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040224-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.